Method for obtaining oncidium and related intergeneric filial generation thereof by using immature embryo in-vitro rescue

A technology for in vitro rescue of young embryos and young embryo rescue, applied in the field of breeding, can solve the problems of embryo abortion, complex background of oncidium, low success rate of hybridization or selfing, etc. Effect

Active Publication Date: 2022-03-22
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, oncidium is native to tropical regions such as Mexico, Brazil, and Bolivia in South America. The varieties on the market mainly rely on imports, which restricts the development of the domestic oncidium industry.
[0003] The background of oncidium is complex, and most of the hybridization or selfing between different oncidium materials is incompatibility, the success rate of hybridization or selfing is low, and after pollination by artificial crossing, some combination pods will show premature aging after a period of growth Phenomenon, embryos are aborted, so that hybrid offspring cannot be obtained, which greatly hinders oncidium germplasm innovation and hybrid breeding work
[0004] In order to improve the efficiency of oncidium germplasm innovation and promote the breeding process of oncidium varieties with independent intellectual property rights, a set of oncidium hybrid immature embryo rescue technology is urgently needed to obtain new oncidium hybrid germplasm resources. There is no method for the rescue of hybrid immature embryos and the acquisition of hybrid germplasm.

Method used

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  • Method for obtaining oncidium and related intergeneric filial generation thereof by using immature embryo in-vitro rescue
  • Method for obtaining oncidium and related intergeneric filial generation thereof by using immature embryo in-vitro rescue
  • Method for obtaining oncidium and related intergeneric filial generation thereof by using immature embryo in-vitro rescue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for obtaining hybrid offspring of Oncidium and its close relatives by using immature embryo rescue technology, comprising the steps of:

[0041](1) Parent material: the female parent selects 'Gomesa Moon Shadow'Tiger Tai', and the male parent selects 'Tiger Tai' (Gomesa Moon Shadow'Tiger Tai');

[0042] (2) Parental self-crossing: select flowers that open or are about to open on the same day, remove buds and flowers that are not open on the same day, pollinate with fresh pollen balls from the male parent, list and record;

[0043] (3) Harvesting and disinfection of fruit pods: respectively select fruit pods 80-90 days after cross-pollination, place the fruit pods in a solution with a ratio of 1:1 of pasteurized liquid and sterile water in an ultra-clean workbench, and Sterilize in a 37°C 110rpm shaker for 20-30 minutes, wash 2-3 times with sterile water in an ultra-clean workbench, and then dry the surface moisture with sterile filter paper;

[0044] (4) Acqui...

Embodiment 2

[0049] A method for obtaining hybrid offspring of Oncidium and its close relatives by using immature embryo rescue technology, comprising the steps of:

[0050] (1) Parent material: Oncidium Jairak Fragance'Pra-Lak' is selected for the female parent, and Gomesa Moon Shadow'Tiger Tai' is selected for the male parent;

[0051] (2) Parental self-crossing: select flowers that open or are about to open on the same day, remove buds and flowers that are not open on the same day, pollinate with fresh pollen balls from the male parent, list and record;

[0052] (3) Harvesting and disinfection of fruit pods: respectively select fruit pods 80-90 days after cross-pollination, place the fruit pods in a solution with a ratio of 1:1 of pasteurized liquid and sterile water in an ultra-clean workbench, and Sterilize in a 37°C 110rpm shaker for 20-30 minutes, wash 2-3 times with sterile water in an ultra-clean workbench, and then dry the surface moisture with sterile filter paper;

[0053] (4)...

Embodiment 3

[0058] A method for obtaining hybrid offspring of Oncidium and its close relatives by using immature embryo rescue technology, comprising the steps of:

[0059] (1) Pollination: Oncidium Jairak Fragrance'Mon-Tho' was selected as the female parent, and 'Tiger Tai' was selected as the male parent;

[0060] (2) Parental self-crossing: select flowers that open or are about to open on the same day, remove buds and flowers that are not open on the same day, pollinate with fresh pollen balls from the male parent, list and record;

[0061] (3) Fruit pod harvesting and disinfection: Take the fruit pods 80-90 days after pollination, the top of the fruit pods is still blue; put the fruit pods in a 1:1 ratio of pasteurized solution and sterile water in an ultra-clean workbench solution, and sterilized in a 190rpm shaker at 37°C for 25 minutes, then washed 2 to 3 times with sterile water in an ultra-clean workbench, and then blotted the surface moisture with sterile filter paper;

[0062]...

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Abstract

The invention discloses a method for obtaining oncidium and related intergeneric filial generation thereof by using immature embryo in-vitro rescue. Comprising the steps of pollination and embryo rescue. According to the method, an oncidium material combination which cannot obtain a hybrid due to abortion of an immature embryo is used as a parent, after cross pollination, the immature embryo is taken out before abortion for immature embryo rescue culture, so that the immature embryo develops into a seedling, and finally a filial generation of the oncidium and a related genus is obtained. The problem that the hybrid F1 generation cannot be obtained due to immature embryo abortion of oncidium and related intergeneric hybridization thereof is solved, a favorable technical guarantee is provided for development of breeding work of oncidium, and the method has positive significance in germplasm resource innovation of oncidium.

Description

technical field [0001] The invention relates to the technical field of breeding, and more specifically relates to a method for obtaining hybrid offspring of oncidium orchids and close relatives thereof by means of in vitro rescue of immature embryos. Background technique [0002] Oncidium (Oncidium) is a perennial flower plant of the Orchidaceae Oncidium genus. Its unique flower shape and beautiful color are known as one of the "five beauties" of cut flowers in the field of flower arrangement. Favored, becoming one of the new favorites of the orchid category. However, oncidium is native to tropical regions such as Mexico, Brazil, and Bolivia in South America. The varieties on the market mainly rely on imports, which restricts the development of the domestic oncidium industry. [0003] The background of oncidium is complex, and most of the hybridization or selfing between different oncidium materials is incompatibility, the success rate of hybridization or selfing is low, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 方能炎樊荣辉叶秀仙罗远华钟淮钦吴建设林榕燕孔兰
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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