Method for improving tissue culture seedling transplanting survival rate of oncidium
A technique for transplanting survival rate and oncidium group is applied in the field of improving the transplanting survival rate of oncidium tissue culture seedlings, which can solve the problems of low survival rate, weak stress resistance, difficult rooting, etc. Growth, no waste of substrate, effect of saving substrate consumption
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Embodiment 1
[0027] A seedling hardening cultivation technique of oncidium tissue culture seedling, its cultivation technique is as follows:
[0028] 1) Selection of cultivation container
[0029] Choose a 72-hole hole tray cultivation container,
[0030] 2) Substrate selection: coconut peat + perlite
[0031] 3) Transplanting technique
[0032] (1) Seedling hardening technology
[0033] Oncidium seedlings are hardened before planting, the purpose is to adapt the tissue cultured seedlings to the external environment and improve the survival rate of transplanting. Move the oncidium tissue culture seedling culture bottle to the greenhouse. The light is controlled at 2000LUX, and it can be transplanted and planted in 10 days.
[0034] (2) Seedling washing: Hold the bottom of the bottle with your left hand, and use the other hand to clamp the rhizome of the seedlings with tweezers, take them out gently without pinching the seedlings, and then wash them in clean water.
[0035] (3) Dilute...
Embodiment 2
[0046] A seedling hardening cultivation technique of oncidium tissue culture seedling, its cultivation technique is as follows:
[0047] 1) Selection of cultivation container
[0048] Choose a 128-hole hole tray cultivation container,
[0049] 2) Substrate selection: Sphagnum moss
[0050] 3) Transplanting technique
[0051] (1) Seedling hardening technology
[0052] Oncidium seedlings are hardened before planting, the purpose is to adapt the tissue cultured seedlings to the external environment and improve the survival rate of transplanting. Move the oncidium tissue culture seedling culture bottle to the greenhouse. The light is controlled at 7000LUX, and it can be transplanted and planted in 30 days.
[0053] (2) Seedling washing: Hold the bottom of the bottle with your left hand, and use the other hand to clamp the rhizome of the seedlings with tweezers, take them out gently without pinching the seedlings, and then wash them in clean water.
[0054] (3) Dilute the dis...
Embodiment 3
[0066] A seedling hardening cultivation technique of oncidium tissue culture seedling, its cultivation technique is as follows:
[0067] 1) Selection of cultivation container
[0068] Use 50 hole tray cultivation container,
[0069] 2) Substrate selection: crushed pine bark
[0070] 3) Transplanting technique
[0071] (1) Seedling hardening technology
[0072] Oncidium seedlings are hardened before planting, the purpose is to adapt the tissue cultured seedlings to the external environment and improve the survival rate of transplanting. Move the oncidium tissue culture seedling culture bottle to the greenhouse. The light is controlled at 4000LUX, and it can be transplanted and planted in 20 days.
[0073] (2) Seedling washing: Hold the bottom of the bottle with your left hand, and use the other hand to clamp the rhizome of the seedlings with tweezers, take them out gently without pinching the seedlings, and then wash them in clean water.
[0074] (3) Dilute the disinfectant...
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