Method for improving tissue culture seedling transplanting survival rate of oncidium

A technique for transplanting survival rate and oncidium group is applied in the field of improving the transplanting survival rate of oncidium tissue culture seedlings, which can solve the problems of low survival rate, weak stress resistance, difficult rooting, etc. Growth, no waste of substrate, effect of saving substrate consumption

Inactive Publication Date: 2018-01-30
三明市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problems of oncidium production tissue culture seedlings, such as difficulty in rooting, serious dis

Method used

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  • Method for improving tissue culture seedling transplanting survival rate of oncidium
  • Method for improving tissue culture seedling transplanting survival rate of oncidium
  • Method for improving tissue culture seedling transplanting survival rate of oncidium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A seedling hardening cultivation technique of oncidium tissue culture seedling, its cultivation technique is as follows:

[0028] 1) Selection of cultivation container

[0029] Choose a 72-hole hole tray cultivation container,

[0030] 2) Substrate selection: coconut peat + perlite

[0031] 3) Transplanting technique

[0032] (1) Seedling hardening technology

[0033] Oncidium seedlings are hardened before planting, the purpose is to adapt the tissue cultured seedlings to the external environment and improve the survival rate of transplanting. Move the oncidium tissue culture seedling culture bottle to the greenhouse. The light is controlled at 2000LUX, and it can be transplanted and planted in 10 days.

[0034] (2) Seedling washing: Hold the bottom of the bottle with your left hand, and use the other hand to clamp the rhizome of the seedlings with tweezers, take them out gently without pinching the seedlings, and then wash them in clean water.

[0035] (3) Dilute...

Embodiment 2

[0046] A seedling hardening cultivation technique of oncidium tissue culture seedling, its cultivation technique is as follows:

[0047] 1) Selection of cultivation container

[0048] Choose a 128-hole hole tray cultivation container,

[0049] 2) Substrate selection: Sphagnum moss

[0050] 3) Transplanting technique

[0051] (1) Seedling hardening technology

[0052] Oncidium seedlings are hardened before planting, the purpose is to adapt the tissue cultured seedlings to the external environment and improve the survival rate of transplanting. Move the oncidium tissue culture seedling culture bottle to the greenhouse. The light is controlled at 7000LUX, and it can be transplanted and planted in 30 days.

[0053] (2) Seedling washing: Hold the bottom of the bottle with your left hand, and use the other hand to clamp the rhizome of the seedlings with tweezers, take them out gently without pinching the seedlings, and then wash them in clean water.

[0054] (3) Dilute the dis...

Embodiment 3

[0066] A seedling hardening cultivation technique of oncidium tissue culture seedling, its cultivation technique is as follows:

[0067] 1) Selection of cultivation container

[0068] Use 50 hole tray cultivation container,

[0069] 2) Substrate selection: crushed pine bark

[0070] 3) Transplanting technique

[0071] (1) Seedling hardening technology

[0072] Oncidium seedlings are hardened before planting, the purpose is to adapt the tissue cultured seedlings to the external environment and improve the survival rate of transplanting. Move the oncidium tissue culture seedling culture bottle to the greenhouse. The light is controlled at 4000LUX, and it can be transplanted and planted in 20 days.

[0073] (2) Seedling washing: Hold the bottom of the bottle with your left hand, and use the other hand to clamp the rhizome of the seedlings with tweezers, take them out gently without pinching the seedlings, and then wash them in clean water.

[0074] (3) Dilute the disinfectant...

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PUM

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Abstract

The invention relates to a method for improving tissue culture seedling transplanting survival rate of oncidium, and belongs to the technical field of agricultural biology. Through selection of a planting matrix, hardening-seedling, seedling washing, fixed planting and other ways, the problems of difficulty in root growing, heavy disease in hardening-seedling and transplanting stages, low survivalrate after taking out of a bottle, weak stress resistance and others of the tissue culture seedling of oncidium can be solved; the method for improving tissue culture seedling transplanting survivalrate of oncidium can improve the transplanting survival rate; the growth recovery of the tissue culture seedling is quick; the disinfectant applied to the method is reasonable in formula, the air-drying time is suitable; the epidemic disease and soft rot in seedling stage of the oncidium can be reduced; moreover, the method is also good for root growth.

Description

technical field [0001] The invention belongs to the field of orchid plant planting. In particular, it relates to a method for improving the transplanting survival rate of oncidium tissue cultured seedlings. Background technique [0002] Oncidium is an epiphytic orchid. It has great ornamental value because of its good branching, rich flower shape and bright color. It is a high-grade potted flower and cut flower material, and is very popular in domestic and foreign markets. The growth conditions of oncidium tissue culture seedlings in the tissue culture room are different from the natural conditions in the field. The tissue culture seedlings have poor resistance, high incidence rate, low survival rate, and poor growth during transplanting, resulting in high cost of seedling cultivation and difficult Obtain a large number of high-quality seedlings for production use. Therefore, it is very important for the industrial production of Oncidium to improve the cultivation technolo...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G24/60A01N57/12A01N59/16A01N43/647A01P1/00
Inventor 李永清叶炜江金兰雷伏贵周建金曹奕鸳莫智龙杨学颜沛沛
Owner 三明市农业科学研究院
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