Detection method of ammonia (ammonia ion) and diagnosis/detection kit of ammonia (ammonia ion)

A technology of ammonia ions and reagents, which is applied in the field of medical/food testing and determination, and can solve the problems of undiscovered blood ammonia determination methods, etc.
CN102466693AInactive Publication Date: 2012-05-23SUZHOU ANJ BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU ANJ BIOTECHNOLOGY CO LTD
Publication Date
2012-05-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a detection method of the ammonia (ammonia ion) content by enzymatic colorimetry and enzyme coupled reaction technology and composition and components of a reagent. The technology principle of the detection method is based on a series of catalytic reactions of ferredoxin-nitrite reductase, nitrite reductase and nitric oxide synthetase. The invention also relates to an ammonia (ammonia ion) diagnosis / detection kit. The detection method provided by the invention has advantages of high sensitivity and small error. Therefore, the detection method and the kit provided by the invention can be widely applied in clinical medicine / food inspection.
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Description

【Technical Field】

[0001] The present invention relates to the technical field of medical / food inspection and measurement. More specifically, the present invention relates to a diagnosis / measurement method of ammonia (ammonia ion) and a kit thereof. 【Background technique】

[0002] Ammonia determination methods include trace diffusion method, ion exchange method, enzymatic method and ammonia electrode method. At present, the most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode.

[0003] The diffusion method is to release NH after alkalization of the specimen 3 , Use acid to titrate the released ammonia, or use Nessler reaction to form brown-yellow amalgam iodide for color comparison. These methods require alkalization, and the formation of endogenous ammonia affects their accuracy and precision. They are rarely used at present; ion exchange methods are more accurate than diffusion methods, with a CV of 8% to 13%; i...

Claims

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