Determination method of ammonia (ammonia ion) and ammonia (ammonia ion) diagnosis / determination kit

A technology of ammonia ions and reagents, which is applied in the field of medical/food testing and determination, and can solve the problems of undiscovered blood ammonia determination methods, etc.
CN102466684AInactive Publication Date: 2012-05-23SUZHOU ANJ BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUZHOU ANJ BIOTECHNOLOGY CO LTD
Publication Date
2012-05-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a determination method of ammonia (ammonia ion) content utilizing enzymatic colorimetric method and couple reaction, and reagent composition and components. Technical principles of the determination method are in the light of a series of catalytic reactions of ferredoxin-nitrite reductase, nitrite reductase and nitric oxide dioxygenase. The invention also relates to an ammonia (ammonia ion) diagnosis / determination kit. The determination method of the present invention has high sensitivity and little error, so the determination method and the kit of the invention can be widely applied to clinic medicine / food examination.
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Description

【Technical field】

[0001] The present invention relates to the technical field of medical / food inspection and determination, more specifically, the present invention relates to ammonia (ammonia ion) diagnosis / measurement method and kit thereof. 【Background technique】

[0002] Ammonia determination methods include micro-diffusion method, ion exchange method, enzymatic method and ammonia electrode method. The most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode.

[0003] The diffusion method is that after the sample is alkalized, NH is released 3 , Titrate the released ammonia with acid, or use the Nessler reaction to form brown-yellow mercuric ammonium iodide for colorimetry. These methods need to be alkalized, and the endogenous ammonia formation will affect the accuracy and precision, so they are rarely used at present; the ion exchange method is more accurate than the diffusion method, and the CV is 8% t...

Claims

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