Genetic engineering chlamydomonas having increased hydrogen producing capacity and application thereof

A technology of genetic engineering and Chlamydomonas, which is applied to genetically engineered Chlamydomonas with increased hydrogen production and its application fields, which can solve the problems of ineffective genetic transformation, unclear mechanism and regulation methods, and restrictions on the application of photosynthetic hydrogen production pathways in microalgae, etc. question

Inactive Publication Date: 2011-09-14
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because photosynthetic water releases oxygen and produces hydrogen are two complex biological metabolic processes that are both contradictory and closely related in microalgal cells, and the mechanism and regulation of these processes are still unclear, resul

Method used

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  • Genetic engineering chlamydomonas having increased hydrogen producing capacity and application thereof
  • Genetic engineering chlamydomonas having increased hydrogen producing capacity and application thereof
  • Genetic engineering chlamydomonas having increased hydrogen producing capacity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Reduce the expression level of FNR1 gene by RNA interference technology, and obtain the mutant Chlamydomonas with increased hydrogen production

[0035] Chlamydomonas reinhardtii (Chlamydomonas reinhardtii) strain CC-400 was purchased from Duke University's algae bank (Chlamycenter);

[0036] The Maa7 / XIR vector was purchased from the Chlamy center of Duke University;

[0037] E.coli DH5α strain was purchased from Bao Biological Engineering (Dalian) Co., Ltd., the product catalog number is D9057.

[0038] 1. Method

[0039] 1. Material cultivation

[0040] Prepare medium:

[0041] TAP liquid medium: NH 4 Cl 0.4g / L; MgSO 4 ·7H 2 O 0.1g / L; CaCl 2 2H 2 O 0.05g / L; K 2 HPO 4 0.108g / L; KH 2 PO 4 0.056g / L; Trisbase 2.423g / L; Hunter’s trace elements (Hunter’s trace elements) 1ml / L, glacial acetic acid 1ml / L, the rest is water;

[0042] Hunter trace elements: EDTA 50g / L; BO 3 h 3 11.4g / L; ZnSO 4 ·7H 2 O 22.0g / L; MnCl 2 4H 2 O 5.06g / L; FeSO 4 ·7H ...

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Abstract

The invention discloses a genetic engineering chlamydomonas having increased hydrogen producing capacity and application thereof. The genetic engineering chlamydomonas provided by the invention is a genetic engineering chlamydomonas which is obtained by inhibiting an expression level of ferredoxin-NADP (Nicotinamide Adenine Dinucleotide Phosphate) reductase coding genes in a starting chlamydomonas. It is determined by gas chromatography that the hydrogen producing capacity of the genetic engineering chlamydomonas is increased by 6-10 times compared with a wild strain after phosphorus deficiency treatment is carried out for 48 hours. The mutant strain is obtained to lay the basis for industrialization of a microalgal photosynthetic hydrogen production technology.

Description

technical field [0001] The invention relates to genetically engineered Chlamydomonas with improved hydrogen production and its application in the field of biotechnology. Background technique [0002] Fossil energy is non-renewable, and excessive use will cause environmental pollution and climate change. Therefore, there is an urgent need to develop renewable and clean new energy sources. Hydrogen is clean and renewable, and its combustion only produces water and huge energy without any pollutants. It is the most ideal clean energy. [0003] Photosynthetic hydrogen production by microalgae is a process in which cyanobacteria and green algae use solar energy to decompose water to produce hydrogen. It has obvious advantages such as regeneration and clean hydrogen production process, and is an effective way to directly use solar energy to bioconvert hydrogen. Because photosynthetic water releases oxygen and produces hydrogen are two complex biological metabolic processes that ...

Claims

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Application Information

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IPC IPC(8): C12N1/13C12N15/113C12N15/63C12N15/11C12P3/00C12R1/89
Inventor 黄芳陈梅孙永乐
Owner INST OF BOTANY CHINESE ACAD OF SCI
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