A genetically engineered bacterium for realizing high-density fermentation and co-production of 2,3-butanediol, its construction method and application

Abstract

The invention discloses a genetically engineered bacterium for realizing high-density fermentation and co-production of 2,3-butanediol, and a construction method and application thereof. Key enzyme genes: α-acetolactate synthase encoding gene, α-acetolactate decarboxylase encoding gene and 2,3-butanediol dehydrogenase encoding gene are integrated into the chromosome of Escherichia coli and constructed. The product acetic acid is reduced, so that it can realize high-density fermentation and co-produce high value-added 2,3-butanediol; in addition, the invention also discloses a method for realizing high-density fermentation and co-producing other compounds and 2,3-butanediol. ‑Butanediol genetically engineered bacteria and its application, in addition to high-density fermentation to produce 2,3‑butanediol, it can also co-produce polyhydroxy fatty acid or functional protein, so as to realize polyhydroxy fatty acid or functional protein, and 2,3‑ The low-cost and high-efficiency co-production of butanediol has important industrial application value.

Description

technical field [0001] The invention belongs to the technical field of biochemical engineering, and in particular relates to a genetically engineered bacterium for realizing high-density fermentation of Escherichia coli and co-production of 2,3-butanediol and its construction method and application. Background technique [0002] Escherichia coli, as the first strain used in industrial production, has become a strain due to its simple structure, clear genetic background, easy culture, fast growth, high expression level of target genes, short culture period, and strong anti-pollution ability. The most commonly used prokaryotic expression system. With the deepening of research in the fields of metabolic engineering and fermentation engineering, Escherichia coli is used to ferment and produce a variety of high-value-added polymers, such as polyhydroxyalkanoate and various functional proteins, such as protease, insulin, and human-like collagen etc. are widely used. [0003] Hig...

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Problems solved by technology

However, the optimization of medium components can only slow down the negative effect of acetic acid on the expression of the target product, but cannot reduce the accumulation of acetic acid, and the change of components will often increase the cost, and will also have an adverse effect on the downstream purification process; reduce the specific growth rate Although the rate can reduce acetic acid, it will also delay the growth of cells and prolong the fermentation period; the dialysis culture method can only treat the symptoms but not the root cause, and cannot avoid the formation of acetic acid, and part of the medium will be lost in the process of removing acetic acid

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