Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of method for cultivating Prorocentrum at high density

A technology for high-density cultivation of Prorodinium algae, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve the problems of small capacity of the light incubator, high energy consumption of the temperature control system, and high price. Achieve the effect of simple operation, small culture volume and high efficiency

Active Publication Date: 2021-07-09
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The light incubator has small capacity and high price, while the walk-in incubator requires high energy consumption of the temperature control system and requires strict aseptic protection for operators

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In the aseptic operation room, suck 19.0L of sterilized f2 medium with a salinity of 28 into a 20L jacketed glass reactor with a sterilized silicone tube, and inoculate the concentration of 5.0×10 according to the volume ratio of 5%. 6 cells / L Prorocentrum PM03, that is, add 1.0L algae seed solution. The reaction kettle was transferred to a routine laboratory, using an air pump to connect a sterilized 0.2μm filter and a silicone tube to ventilate at 3L / min, the reaction kettle speed was 80rpm / min, the light was 5000lux, the light / dark cycle was 14h / 10h, and the temperature of the jacketed circulating water was Controlled 22-25 ℃, cultivated for 15 days. Then in the aseptic operation room, release most of the cultured algae liquid from the discharge port of the reactor, and reserve a certain volume for inoculation in the next culture cycle. Using the Sedgewick-Rafter plate to count the cells under the microscope, the cell density of the cultured algal fluid was 8.97×10 ...

Embodiment 2

[0026] Continuation of Example 1, in the aseptic operating room, the cell density retained in a 20L jacketed glass reactor is 8.97 × 10 7 1.5L of Prorodinium PM03 algae seed solution in cells / L, inhale and add 18.5L of sterilized f2 medium with a salinity of 25 through a sterilized silicone tube. The reaction kettle was transferred to a routine laboratory, and the air pump was connected to a sterilized 0.2μm filter and a silicone tube to ventilate at 3L / min, the reaction kettle speed was 80rpm / min, the light was 4000lux, the light / dark cycle was 14h / 10h, and the temperature of the jacketed circulating water was Controlled 22-25 ℃, cultivated for 15 days. Then in the aseptic operation room, release most of the cultured algae liquid from the discharge port of the reactor, and reserve a certain volume for inoculation in the next culture cycle. Using the Sedgewick-Rafter plate to count the cells under the microscope, the cell density of the cultured algal fluid was 1.38×10 8 cel...

Embodiment 3

[0028] Continuation of Example 2, in the aseptic operating room, the cell density retained in a 20L jacketed glass reactor is 1.38 × 10 8 cells / L of Prorodinium PM03 algae seed solution 2.0L, inhale and add 18.0L sterilized f2 medium with a salinity of 25 through a sterilized silicone tube. The reaction kettle was transferred to a conventional laboratory, and the air pump was connected to a sterilized 0.2μm filter and a silicone tube to ventilate at 3L / min, the reaction kettle speed was 80rpm / min, the light was 3000lux, the light / dark cycle was 14h / 10h, and the temperature of the jacketed circulating water was Controlled 22-25 ℃, cultivated for 15 days. Then in the aseptic operation room, 18 L of most of the cultured algae liquid was released from the discharge port of the reactor, and the cell density of the cultured algae liquid was 2.05 × 10 by using the Sedgewick-Rafter plate to count the cells under the microscope. 8cells / L; The remaining 2.0L algae seed solution is used...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for high-density cultivation of Prorodinova, which comprises the steps of: (a) inoculating the Prorodinova seed solution in a 10-50L clip containing sterilized f2 medium in an aseptic operation room; In the jacketed glass reactor, the salinity of the f2 culture medium is 20-28; (b) transferred to a conventional laboratory, introduced sterilized air, controlled illuminance to 3000-5000lux, light / dark cycle 14h / 10h, clamped The temperature of the circulating water is controlled at 22-25°C, and the culture is carried out for 10-20 days; (c) In the aseptic operation room, most of the cultivated algae liquid is released from the outlet of the reactor, and freshly sterilized f2 is added to cultivate the base of the reactor , transferred to a routine laboratory to continue the next culture cycle under the same conditions as in step b. The whole culture process of the present invention is easy to operate, the culture volume is small and the cell density is extremely high, and the total extract can be obtained more efficiently; and compared with large-volume glass bottle or glass tank culture, it does not need to be cultured in a sterile and constant temperature culture room all the time ,Low energy consumption.

Description

technical field [0001] The invention belongs to the field of cultivating marine micro-unicellular algae, in particular to a method for cultivating Prorocentella in high density. Background technique [0002] Marine organisms are rich in secondary metabolites with novel structures and significant biological activities, so they have become the most potential sources of new drugs today. In addition to marine invertebrates such as sponges, corals, and sea squirts, and marine microorganisms such as actinomycetes and fungi, Marine microalgae resources are also one of the hot topics in the research of marine natural medicinal chemistry; the polyketides, polyethers, and alkaloids produced by their metabolism are classified as neurotoxic shellfish because of their specific toxicological characteristics on mammals. 8 types of marine biotoxins, including paralytic shellfish poisoning, diarrhea shellfish poisoning, and cyclic imine toxins, whose monitoring is the core content of aquatic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 樊成奇田晓清乔玉宝唐莹莹陆亚男马丽艳
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com