Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes

A technology for pine wood nematode disease and pine wood nematode, which is applied in the directions of nematicides, botanical equipment and methods, chemicals for biological control, etc.

Inactive Publication Date: 2012-08-15
南京绿海生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the felling is not complete, and it happens every year. ②The trunk is injected with nematicides to protect the precious pine trees
The disadvantage of this method is that it is cumbersome to use and causes great damage to trees
③Using par

Method used

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  • Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes
  • Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes
  • Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes

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Embodiment 1

[0043] Embodiment 1: The implementation process of the method for preventing and treating pine wood nematode disease by using the bacteria on the surface of pine wood nematode provided by the embodiment of the present invention is as follows figure 2 shown.

[0044] The specific details are as follows:

[0045] S201: Select a material. Two batches of wood packing boxes imported from the United States were tested. The aseptic pine wood nematodes for the test were the Chinese Zhejiang pine wood nematode strains. The reference bacterial strains were Staphylococcus aureus and Escherichia coli. provided by the room;

[0046] S202: Isolation of nematodes and bacteria. The nematodes in the wood block were separated by the Baermann funnel method, and the bacteria on the surface of the nematodes were separated by the dilution separation method.

[0047] S203: Preparation of bacterial cell-free culture medium. Bacteria were inserted into beef extract peptone liquid medium, and cul...

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Abstract

The invention is suitable for the field of nematode disease control and provides a method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes. The method comprises the steps of: spraying American bacterium suspension liquid into woodlands: spraying once in May for two years in a row, wherein the body surface pathogenic bacteria of the pine wood nematodes are replaced with American bacteria in two or three years so as to control the prevalence of woodland pine wood nematode diseases in China. According to the invention, the biological control is carried out by utilizing the replacement of ecological niche of the body surface bacteria of the pine wood nematodes and bacterial strains isolated and screened from body walls of the American pine wood nematodes. In the method, the biocontrol bacteria sprayed into the woodlands have the characteristics of low pathogenicity, strong reproduction and replacement capabilities and the like and are capable of rapidly replacing the body surface strong pathogenic bacteria of the pine wood nematodes in China to ensure that the pine wood nematodes lose the pathogenic ability and the purpose of controlling the prevalence of pine wood nematode diseases is achieved. The method has the advantages of simpleness, practicability, easiness in popularization and application, lower cost, good and thorough effect and the like.

Description

technical field [0001] The invention belongs to the technical field of nematode disease prevention and control, and in particular relates to a method for preventing and treating pine wood nematode disease by using bacteria on the surface of pine wood nematodes. Background technique [0002] Pine wood nematode (Pine wild disease) is the world's number one disease of forest trees, which can cause pine forests to wither and die in patches. Since it was introduced into my country in 1982, the disease has been raging wildly in the north and south of our country. Wherever he went, the pine forest was withered and yellow. According to incomplete statistics, the annual disease occurrence area in my country is about 4000hm2, resulting in the death of more than 150,000 pine trees and about 5000m3 of wood loss. The annual national expenditure on disease prevention and control also reaches 220 million yuan. Although the research on pine wood nematode disease in our country has been go...

Claims

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Application Information

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IPC IPC(8): A01G13/00A01N63/00A01P5/00
Inventor 韩正敏贲爱玲曾腓力乔雪娟刘侃诚傅军孙京天朋金和
Owner 南京绿海生物科技有限公司
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