Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes
A technology for pine wood nematode disease and pine wood nematode, which is applied in the directions of nematicides, botanical equipment and methods, chemicals for biological control, etc.
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[0043] Embodiment 1: The implementation process of the method for preventing and treating pine wood nematode disease by using the bacteria on the surface of pine wood nematode provided by the embodiment of the present invention is as follows figure 2 shown.
[0044] The specific details are as follows:
[0045] S201: Select a material. Two batches of wood packing boxes imported from the United States were tested. The aseptic pine wood nematodes for the test were the Chinese Zhejiang pine wood nematode strains. The reference bacterial strains were Staphylococcus aureus and Escherichia coli. provided by the room;
[0046] S202: Isolation of nematodes and bacteria. The nematodes in the wood block were separated by the Baermann funnel method, and the bacteria on the surface of the nematodes were separated by the dilution separation method.
[0047] S203: Preparation of bacterial cell-free culture medium. Bacteria were inserted into beef extract peptone liquid medium, and cul...
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