Method for preventing and treating pine wilt disease by using American pine wood nematode body surface bacteria

Abstract

The invention discloses a method for preventing and treating the pine wilt disease by using American pine wood nematode body surface bacteria. The method comprises the following steps: separation of nematodes and bacteria: separating nematodes in a wood block to be tested by adopting a Belman funnel method, and separating bacteria on body surface of the nematodes by adopting a dilution separation method; preparation of a bacterial suspension; preparation of a bacterial cell-free culture solution; and application of a bacterial cell-free filtrate: applying the bacterial cell-free filtrate according to the size of the diameter at breast height of a pine tree, wherein the use frequency of the bacterial cell-free filtrate is 2 years per time. According to the method for preventing and treating the pine wilt disease by using the American pine wood nematode body surface bacteria, a pesticide application amount is determined according to the size of the diameter at breast height of the pine tree, and the ecological niche of the pine wood nematode body surface bacteria is replaced, so the prevalence rate of the pine tree is effectively reduced, and the survival rate of the pine tree is improved; a prevention and treatment period is prolonged, and the disease can be prevented for 2 to 3 years as the bacterial cell-free filtrate is applied once; and the method is easy to operate and low in cost, and the survival rate of the pine tree reaches 99% when the pine tree is treated with the method.

Description

technical field [0001] The invention relates to the technical field of prevention and treatment of pine wood nematode disease, in particular to a method for preventing and treating pine wood nematode disease by using bacteria on the surface of pine wood nematode. Background technique [0002] Pine wood nematode disease is a devastating disease of pine trees, commonly known as "cancer of pine trees". Is a tree species. The disease has occurred in the United States, Canada, Mexico, Japan, South Korea, China, etc., and Japan is the most serious. Pine wood nematode disease invaded Hong Kong, China in the 1980s and almost wiped out the extensive masson pine forests in Hong Kong. In 1982, pine wood nematode disease was first discovered in Zhongshan Mausoleum, Nanjing, China, and then successively formed several disease centers in Anhui, Shandong, Zhejiang, Guangdong and other places, and spread to the surrounding areas, spreading into disasters in some areas of these provinces, ...

AI Technical Summary

Problems solved by technology

[0004] At present, the outbreak of pine wood nematode at home and abroad is serious and the loss is huge

The prevention and control of pine wood nematode disease mostly adopts physical, chemical, biological and other technical means to control the population density of Monochamus alternatus, such as the use of traps, black light lamps, wood traps and other methods for physical control, but this physical control method is widely promoted. It is more difficult; artificial and aircraft spraying or tree trunk injection are used for chemical prevention and control. Generally, agents with contact killing, stomach poisoning, systemic effect and strong permeability are used. The cost of chemical prevention and control is relatively large and it is easy to cause secondary pollution; and Biological control is usually based on the release of pathogenic microbial powders such as Beauveria bassiana and Metarhizium anisopliae, and natural enemy insects such as Anthracite and Swollen-legged beetles. This biological control method is relatively slow

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