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Bacillus amyloliquefaciens strain and application thereof

A technology for dissolving amyloliquefaciens spores and strains, which is applied in the field of Bacillus amyloliquefaciens Lx-11 strains and biocontrol agents to achieve good control effects and the effects of human and animal safety.

Inactive Publication Date: 2012-01-04
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of Bacillus amyloliquefaciens to control rice bacterial diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation and Preservation of Bacillus amyloliquefaciens Strains

[0026] In February 2009, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain was isolated by gradient dilution method in the paddy and dry rotation experiment field of Jiangsu Academy of Agricultural Sciences. The specific method is: take 10g of the soil collected from the paddy and dry crop rotation test field of Jiangsu Academy of Agricultural Sciences, add it into a triangular flask filled with 90mL sterilized water, and cultivate it with shaking for 30min (150r / min) to make a 1:10 (soil: water ) suspension, and then take 1mL of the suspension and place it in 9mL sterile water to make 10 -2 Dilute the suspension, and so on, to make 10 -5 , 10 -6 Dilute the suspension; draw 200 μL each, add it to the YPGA medium plate, spread it evenly with a coating bar, and repeat each treatment 3 times. The above culture dish was placed in a constant temperature incubator at 28°C for 1-2 days. Select ...

Embodiment 2

[0031] Preparation of Fermented Strains of Biocontrol Bacteria Lx-11

[0032] 1. Strain activation: transplant the freeze-dried and preserved Lx-11 strain to the YPGA slant, and cultivate it at 28°C for 48 hours.

[0033] Preparation of YPGA slope: Weigh 5.0g of yeast extract, 5.0g of tryptone, 5.0g of glucose, and 17.0g of agar; put it into 1000ml of water, heat and mix well, then adjust the pH to 6.8-7.2, and then pack it into a test tube after proper cooling In the medium, the filling volume is 1 / 5-1 / 6 of the test tube, plug the test tube mouth with a stopper, sterilize with damp heat at 121°C for 30 minutes, take out the test tube while it is hot and set it on a slope until it cools down for later use.

[0034] 2. Preparation of fermented strains: Take a ring of activated Lx-11 bacteria and transfer them into 300ml YPG culture solution packed in a 1000ml Erlenmeyer bottle, and cultivate them at 120 rpm and 28°C for 48 hours. The bacteria content in the culture solution can...

Embodiment 3

[0037] Fermentation procedure and quality control of Lx-11 biocontrol agent

[0038] 1. Fermentation procedure:

[0039] Transfer the cultured fermentation strains into the fermentation medium of the seed tank for fermentation and cultivation. The fermentation time in the seed tank is 12 to 16 hours. After 4 hours of transferring the fermentation strains, the fermentation liquid is fermented every 2 hours. Detection, when the growth of the fermented liquid bacteria is relatively full and spores are about to appear, immediately transfer the fermented liquid in the seed tank to the fermentation medium of a large-scale fermenter for fermentation and cultivation. The fermentation time in the large-scale fermenter is 24 to 36 hours. After 4 hours of fermentation, test the fermentation quality of the fermentation broth every 2 hours, observe the bacteria content, and measure the pH of the fermentation broth. When there are many foams, an appropriate amount of defoaming agent can be ...

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Abstract

The invention relates to a bacillus amyloliquefaciens strain, which is characterized in that the collection No. of the strain is CGMCC No.3789; and the 16S rRNA of the strain accesses to GenBank, with accession No. of HQ179100. The strain is used for controlling rice bacterial leaf streak. The strain has the following advantages: the strain has good control effect on the rice bacterial leaf streak, is free of toxin and pathogenicity, is harmless to people and livestock and is environment-friendly; the prepared biocontrol bacteria or the compound biocontrol bacteria formed by compounding the prepared biocontrol bacteria and bismerthiazol are sprayed after being diluted, so that reasonable distribution of the microfloras in the ecological environment around the root leaves of the rice can be controlled and managed and the living environment of the pathogenic bacteria is worsened to form a rice ecosystem with biodiversity, thus effectively and enduringly controlling prevalence of the rice bacterial leaf streak.

Description

technical field [0001] The invention relates to a Bacillus amyloliquefaciens Lx-11 strain, a biocontrol bacterial agent prepared by using the bacterial strain and an application of the bacterial agent. The invention belongs to the field of biological control of rice bacterial stripe disease by using beneficial microorganisms. Background technique [0002] Rice bacterial streak disease is an important disease in rice production in my country, especially in the south, and it is also one of the important limiting factors to ensure the safety of rice production in my country. At present, the disease occurs in an area of ​​more than 20 million mu all year round, causing a loss of 5-10% of rice yield, and even more than 30% in severe cases. [0003] In the 1970s and 1980s, with the large-scale promotion and application of hybrid rice in the country, rice bacterial diseases swept across the land of China, causing the annual loss of rice production to reach more than 20%, becoming ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02C12R1/07A01P1/00C12N1/20
Inventor 陈志谊刘永峰张荣胜
Owner JIANGSU ACAD OF AGRI SCI
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