Application of gene FoRnt in regulation and control of pathogenicity of fusarium oxysporum

Abstract

The invention discloses application of a gene FoRnt in regulation and control of pathogenicity of fusarium oxysporum, and belongs to the field of plant genetic engineering. The method comprises the following steps: constructing a gene knockout vector, and introducing the gene knockout vector into a fusarium oxysporum protoplast; knocking out the gene from fusarium oxysporum by using a homologous recombination method to obtain a knockout mutant delta FoRnt; constructing a gene back-supplementing vector, and introducing the gene back-supplementing vector into a delta FoRnt protoplast to obtain aback-supplementing mutant delta FoRnt-com. It is proved by test that delta FoRnt has no significant difference from wild type in the aspects of spore morphology, mycelium morphology, high permeability resistance, oxidative stress resistance and the like; however, the deficiency of FoRnt leads to the significant reduction of the pathogenicity; the invention proves that the FoRnt is necessary for the pathogenicity of the fusarium oxysporum. The research is helpful for deeply illuminating the pathogenic molecular mechanism of fusarium oxysporum, and provides a target gene for developing effective bactericides.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and in particular relates to the application of a gene FoRnt in regulating the pathogenicity of Fusarium wilt of banana. Background technique [0002] Fusarium wilt (Fusarium wilt) is one of the most important diseases in banana production, which poses a serious threat to the banana industry in my country. The pathogen is Fusarium oxysporum f.sp.cubense (Foc). Banana Fusarium wilt is a systemic vascular soil-borne disease that can destroy banana vascular bundles and cause plant death. Foc can be divided into three physiological races, and the main ones that endanger the banana planting area in my country are race 1 (Foc1) and race 4 (Foc4). Among them, Foc4 is the most harmful to bananas in my country, and can infect almost all varieties of bananas. The pathogenic mechanism of Foc is still unclear. It is of great significance to fully excavate Foc pathogenicity-related genes and carry ...

AI Technical Summary

Problems solved by technology

RNase T2 was first discovered in Aspergillus oryzae. It is a single-strand specific transfer-type endonuclease, which has a wide range of biological functions, such as nucleic acid removal, self-RNA degradation, etc.; but its biology in Fusarium wilt function is not clear

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