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Application of Magnaporthe grisea gene mormd1 in regulating the pathogenicity of Magnaporthe grisea

A technology of rice blast fungus and rice blast, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., and can solve problems such as unknown functions

Active Publication Date: 2021-05-28
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many DUFs are highly conserved and presumably play an important role in the biological functions of fungi, but their specific functions are unknown

Method used

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  • Application of Magnaporthe grisea gene mormd1 in regulating the pathogenicity of Magnaporthe grisea
  • Application of Magnaporthe grisea gene mormd1 in regulating the pathogenicity of Magnaporthe grisea
  • Application of Magnaporthe grisea gene mormd1 in regulating the pathogenicity of Magnaporthe grisea

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Embodiment 1

[0037] 1. Experimental materials

[0038] 1.1 Tested strains and plants

[0039] Magnaporthe oryzae race is the dominant race ZC in Guangdong Province 13 , the tested rice was indica rice line CO39 (without known blast resistance gene).

[0040] 1.2 Host bacteria and plasmid vector

[0041] The cloning vector is pMD18-T vector, and the gene knockout vector is the filamentous fungal expression vector pCT74.

[0042] 2. Experimental method

[0043] 2.1 Construction of Magnaporthe grisea knockout vector

[0044] Schematic diagram of the construction of blast fungus MoRMD1 gene knockout vector figure 1 shown. Sequences with a length of about 1500 bp were selected at the upstream and downstream of the MoRMD1 gene, and primers were designed (Table 1).

[0045] Table 1 Amplification primers for homology arm A fragment and homology arm B fragment of Magnaporthe grisea MoRMD1 gene

[0046] Primer name Primer sequence 5'-3' Restriction sites MoRMD1-AF GG GG...

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Abstract

The invention discloses the application of a blast fungus gene MoRMD1 in regulating the pathogenicity of blast fungus, belonging to the field of plant genetic engineering. The invention constructs a gene knockout vector and introduces it into the protoplast of Magnaporthe oryzae; knocks out the gene MoRMD1 from Magnaporthe oryzae by using a homologous recombination method to obtain a knockout mutant ΔMoRMD1; the obtained Magnaporthe oryzae knockout The mutants are defective in appressorium formation, and MoRMD1 is important for maintaining cell wall integrity. Pathogenicity tests showed that the deletion of MoRMD1 significantly reduced the virulence of Magnaporthe oryzae, and no obvious lesions could be formed on rice leaves. The present invention demonstrates that MoRMD1 is required for appressorium formation, maintenance of cell wall integrity and pathogenicity of M. oryzae. Our research helps to elucidate the pathogenic molecular mechanism of Magnaporthe grisea and provides target genes for the development of effective fungicides.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, in particular to the application of a blast fungus gene MoRMD1 in regulating the pathogenicity of blast fungus. Background technique [0002] Rice blast caused by Magnaporthe oryzae is an important disease in rice production. The rice economic loss caused by rice blast is about 66 billion US dollars every year in the world, and the lost rice can provide food for 60 million people. The infection process of rice blast fungus mainly includes: (1) conidia spread with wind and rain, and adhere to the surface of rice leaves; (2) conidia germinate and form germ tubes; (3) germ tubes differentiate to form attached (4) appressorium differentiates to form infection nails; (5) infection nails penetrate the host cells, form infection hyphae in the host cells, and spread between cells. Among them, the key process for the successful infection of rice by blast fungus lies in the formation of a highly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/37C12N15/31A01N57/16A01P3/00
CPCA01N57/16C07K14/37
Inventor 李云锋聂燕芳田苗李华平王振中
Owner SOUTH CHINA AGRI UNIV
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