Application of gene fornt in regulating the pathogenicity of Fusarium wilt of banana

A technology of banana fusarium wilt and banana wilt, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of unclear biological function of banana fusarium wilt

Active Publication Date: 2022-03-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

RNase T2 was first discovered in Aspergillus oryzae. It is a single-strand specific transfer-type endonuclease, which has a wide range of biological functions, such as nucleic acid removal, self-RNA degradation, etc.; but its biology in Fusarium wilt function is not clear

Method used

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  • Application of gene fornt in regulating the pathogenicity of Fusarium wilt of banana
  • Application of gene fornt in regulating the pathogenicity of Fusarium wilt of banana
  • Application of gene fornt in regulating the pathogenicity of Fusarium wilt of banana

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Embodiment 1

[0031] 1. Experimental materials

[0032] 1.1 Tested strains and plants

[0033] The race of Fusarium wilt was Foc4, and the tested banana variety was Brasilia Cavendish (AAA).

[0034] 1.2 Host bacteria and plasmid vector

[0035] The host bacterium is Escherichia coli DH5α, the cloning vector is pMD18-T vector, the gene knockout vector is the binary vector pCT74, and the gene complementation vector is pCTZN (transformed by our laboratory on the basis of the pCT74 plasmid, that is, the SGFP and The hph gene was replaced by the bleomycin (Zeocin) gene), which was also disclosed in the patent "201910986131.4, the application of gene FoPDCD5 in regulating the pathogenicity of Fusarium wilt of banana".

[0036] 2. Experimental method

[0037] 2.1 Amplification of upstream and downstream homologous fragments of Fusarium wilt gene FoRnt

[0038] The construction of Fusarium wilt FoRnt gene knockout vector is as follows: figure 1 shown. Sequences with a length of about 1500 bp...

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Abstract

The invention discloses the application of a gene FoRnt in regulating the pathogenicity of Fusarium wilt of banana, and belongs to the field of plant genetic engineering. The present invention constructs a gene knockout vector and introduces it into the protoplast of Fusarium wilt of banana; uses homologous recombination to knock out the gene from Fusarium wilt of banana to obtain a knockout mutant ΔFoRnt; Introduce ΔFoRnt protoplasts to obtain the complementation mutant ΔFoRnt‑com. Tests have shown that ΔFoRnt has no significant difference compared with the wild type in terms of spore morphology, hyphae morphology, resistance to high osmosis, oxidative stress, etc.; but the deletion of FoRnt has caused a significant reduction in its pathogenicity; the present invention proves that FoRnt is a banana Necessary for pathogenicity of Fusarium wilt. Our research helps to further elucidate the pathogenic molecular mechanism of Fusarium wilt, and provides target genes for the development of effective fungicides.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and in particular relates to the application of a gene FoRnt in regulating the pathogenicity of Fusarium wilt of banana. Background technique [0002] Fusarium wilt (Fusarium wilt) is one of the most important diseases in banana production, which poses a serious threat to the banana industry in my country. The pathogen is Fusarium oxysporum f.sp.cubense (Foc). Banana Fusarium wilt is a systemic vascular soil-borne disease that can destroy banana vascular bundles and cause plant death. Foc can be divided into three physiological races, and the main ones that endanger the banana planting area in my country are race 1 (Foc1) and race 4 (Foc4). Among them, Foc4 is the most harmful to bananas in my country, and can infect almost all varieties of bananas. The pathogenic mechanism of Foc is still unclear. It is of great significance to fully excavate Foc pathogenicity-related genes and carry ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C07K14/37A01N63/60A01P3/00
CPCC07K14/37A01N63/60
Inventor 聂燕芳何艳秋李云锋李华平
Owner SOUTH CHINA AGRI UNIV
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