Application of magnaporthe oryzae gene MoRMD1 in regulating and controlling magnaporthe oryzae pathogenicity

A rice blast fungus and virulence technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems such as unknown function

Active Publication Date: 2019-08-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many DUFs are highly conserved and presumably play an important role in the biological functions of fungi, but their specific functions are unknown

Method used

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  • Application of magnaporthe oryzae gene MoRMD1 in regulating and controlling magnaporthe oryzae pathogenicity
  • Application of magnaporthe oryzae gene MoRMD1 in regulating and controlling magnaporthe oryzae pathogenicity
  • Application of magnaporthe oryzae gene MoRMD1 in regulating and controlling magnaporthe oryzae pathogenicity

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Embodiment 1

[0037] 1. Experimental materials

[0038] 1.1 Tested strains and plants

[0039] Magnaporthe oryzae race is the dominant race ZC in Guangdong Province 13 , the tested rice was indica rice line CO39 (without known blast resistance gene).

[0040] 1.2 Host bacteria and plasmid vector

[0041] The cloning vector is pMD18-T vector, and the gene knockout vector is the filamentous fungal expression vector pCT74.

[0042] 2. Experimental method

[0043] 2.1 Construction of Magnaporthe grisea knockout vector

[0044] Schematic diagram of the construction of blast fungus MoRMD1 gene knockout vector figure 1 shown. Sequences with a length of about 1500 bp were selected at the upstream and downstream of the MoRMD1 gene, and primers were designed (Table 1).

[0045] Table 1 Amplification primers for homology arm A fragment and homology arm B fragment of Magnaporthe grisea MoRMD1 gene

[0046] Primer name Primer sequence 5'-3' Restriction sites MoRMD1-AF GG GG...

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Abstract

The invention discloses an application of a magnaporthe oryzae gene MoRMD1 in regulating and controlling the magnaporthe oryzae pathogenicity, and belongs to the field of plant gene engineering. According to the invention, a gene knock-out vector is constructed and introduced into a magnaporthe oryzae protoplast; the gene MoRMD1 is knocked out from the magnaporthe oryzae by using a homologous recombination method so as to obtain a knock-out mutant DELTA.MoRMD1; and the obtained magnaporthe oryzae knock-out mutant o has defects in appressorium formation, and MoRMD1 plays an important role in maintaining the integrity of cell walls. Pathogenicity tests show that the deletion of the MoRMD1 obviously reduces the toxicity of the magnaporthe oryzae, and obvious disease spots cannot be formed onrice leaves. The present invention demonstrates that MoRMD1 is necessary for forming magnaporthe oryzae appressoria and maintaining cell wall integrity and pathogenicity. The research is helpful to deeply elucidating the pathogenic molecular mechanism of magnaporthe oryzae and providing target genes for the development of effective fungicides.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, in particular to the application of a blast fungus gene MoRMD1 in regulating the pathogenicity of blast fungus. Background technique [0002] Rice blast caused by Magnaporthe oryzae is an important disease in rice production. The rice economic loss caused by rice blast is about 66 billion US dollars every year in the world, and the lost rice can provide food for 60 million people. The infection process of rice blast fungus mainly includes: (1) conidia spread with wind and rain, and adhere to the surface of rice leaves; (2) conidia germinate and form germ tubes; (3) germ tubes differentiate to form attached (4) appressorium differentiates to form infection nails; (5) infection nails penetrate the host cells, form infection hyphae in the host cells, and spread between cells. Among them, the key process for the successful infection of rice by blast fungus lies in the formation of a highly ...

Claims

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Application Information

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IPC IPC(8): C07K14/37C12N15/31A01N57/16A01P3/00
CPCA01N57/16C07K14/37
Inventor 李云锋聂燕芳田苗李华平王振中
Owner SOUTH CHINA AGRI UNIV
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