Building and application of M protein three-amino acid site-mutated vesicular stomatitis virus (VSV) carrier for pigs

A virus vector, three amino acid technology, applied in the direction of using the vector to introduce foreign genetic material, antiviral agents, medical preparations containing active ingredients, etc., to achieve the effects of reducing pathogenicity, improving safety, and good application prospects

Inactive Publication Date: 2015-11-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, judging from the results, although VSVΔM51 has been weakened to a certain extent compared with the wild-type VSV virus, it still shows a certain degree of pathogenicity, and further modification is still needed to improve its safety.

Method used

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  • Building and application of M protein three-amino acid site-mutated vesicular stomatitis virus (VSV) carrier for pigs
  • Building and application of M protein three-amino acid site-mutated vesicular stomatitis virus (VSV) carrier for pigs
  • Building and application of M protein three-amino acid site-mutated vesicular stomatitis virus (VSV) carrier for pigs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] This example relates to the construction of a novel porcine vesicular stomatitis virus vector with mutations at three amino acid sites in the matrix protein (M).

[0037] 1.1 Cells and Reagents

[0038] In this embodiment, VSV XN2 , VSVΔM51, and poxvirus vTF7-3 were preserved by our laboratory; BHK21 and Vero cells were preserved by our laboratory; VSV virus (VSV-GFP) expressing green fluorescent protein GFP was also prepared and preserved by our laboratory according to the literature (SchnellMJ, BuonocoreL, KretzschmarE, JohnsonE, RoseJK. Foreignglycoproteinsexpressedfromrecombinantvesicularstomatitisvirusesareincorporatedefficientlyintovirusparticles.ProcNatlAcadSciUSA.1996,93(21):11359-65). XbaI and MluI restriction endonucleases were purchased from NEB Company, DMEM for cell culture was purchased from Thermo Company, fetal bovine serum was purchased from GBICO Company, cell transfection liposome Lipofectamine2000 was purchased from Invitrogen Company, PFU high-fide...

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Abstract

The invention provides building and application of an M protein three-amino acid site-mutated vesicular stomatitis virus (VSV) carrier for pigs. The VSV carrier is matrix protein (M) three-amino acid site-mutated recombinant virus VSV-MT, the 51st-site methionine of the virus M protein is knocked out, the 221st-site valine of the virus M protein is mutated into phenylalanine, and the 226th-site glycine of the virus M protein is mutated into arginine. The VSV carrier is extremely low in pathogenicity, high in safety, capable of effectively stimulate a body to produce protective immune response, applicable to vaccine or vaccine carrier preparation, and promising in application prospect.

Description

technical field [0001] The invention relates to the construction and application of a novel vector of porcine vesicular stomatitis virus with mutations in three amino acid sites of matrix protein (M). Background technique [0002] Vesicular stomatitis in domestic animals is caused by vesicular stomatitis virus (VSV). It is an acute and highly pathogenic infectious disease that can cause harm to animal husbandry worldwide. It is listed as a second-class animal in my country. Infectious diseases, currently there is no effective vaccine to prevent. [0003] Vesicular stomatitis is caused by vesicular stomatitis virus (VSV) and can be fatal in cattle and pigs. Infected animals present with fever, lethargy, and decreased appetite; vesicular lesions appear on the mouth, teats, between the toes, and hooves, and pot-shaped rings on the legs. The blisters are easy to rupture, exposing granulation tissue, showing red erosion, and usually fully heal within 7 to 10 days. Vesicular sto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/86C12N15/66A61K39/205A61P31/14
Inventor 孙涛肖贤方心葵
Owner SHANGHAI JIAO TONG UNIV
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