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Fig leaf extract with bacterium colony induction quenching activity, and use thereof

A quorum sensing and fig leaf technology is applied in activity screening and in the field of bacterial quorum sensing inhibitor preparation and fig leaf extract preparation. The effect of bacillus violetin production

Active Publication Date: 2016-06-15
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no literature has reported the quorum sensing inhibitory activity of its extracts so far.

Method used

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  • Fig leaf extract with bacterium colony induction quenching activity, and use thereof
  • Fig leaf extract with bacterium colony induction quenching activity, and use thereof
  • Fig leaf extract with bacterium colony induction quenching activity, and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0016] Embodiment 1: A preparation method of fig leaf extract with bacterial quorum sensing quenching activity

[0017] Naturally air-dried fig leaves at room temperature were crushed into foam, weighed 1g of the raw material and placed in a conical shaker flask, added 20ml of dichloromethane solvent, ultrasonicated for 30min, and then placed on a shaker at 28°C for 48h for extraction. Filtrate and filter residue were obtained by filtration; the filter residue was extracted twice with 20ml dichloromethane solvent according to the above-mentioned method, and a total of 3 filtrates were obtained, combined, concentrated in vacuo, reversed-phase silica gel C 18 The crude extract of fig leaf extract was obtained after pre-column treatment.

Embodiment approach 2

[0018] Embodiment 2: The difference between Embodiment 2 and Embodiment 1 is that the extraction reagent used is methanol, and other steps are the same as Embodiment 1.

Embodiment approach 3

[0019] Embodiment 3: The difference between Embodiment 3 and Embodiment 1 or 2 is that the extraction reagent used is n-hexane, and other steps are the same as Embodiment 1.

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Abstract

The invention provides a fig leaf extract with bacterium colony induction quenching activity, and a use thereof, and concretely relates to a preparation method of a fig leaf crude extract, a bacterium colony induction quenching activity screening method, and an application of the extract in the preparation of bacterium colony induction inhibitors. The bacterium colony induction quenching activity of the fig leaf extract is reported for the first time in the invention. The fig leaf crude extract prepared through extraction using organic solvents with different polarities has an inhibition effect on Chromobacterium violaceum and Pseudomonas aeruginosa colony induction system, wherein a dichloromethane extract has strongest colony induction system quenching activity. The crude extract can substantially reduce the output of colony induction mediated Chromobacterium violaceum purpurin and the flocking motion of the Pseudomonas aeruginosa and weaken the pesticide resistance of pathogenic bacteria, and can be used to prepare novel bacterium colony induction inhibitors, so the fig leaf extract has wide application prospect in prevention and control of bacterial infective diseases.

Description

technical field [0001] The invention belongs to the field of development and utilization of plant active ingredients, and in particular relates to the preparation, activity screening and application of a fig leaf extract with bacterial quorum sensing quenching activity and preparation of bacterial quorum sensing inhibitors. Background technique [0002] With the extensive use and abuse of antibiotics, a wide range of drug-resistant strains have emerged clinically, making bacterial drug resistance an increasingly serious worldwide problem. Traditional antibacterial drugs can easily cause bacteria to develop drug resistance due to survival pressure. Therefore, the existing antibacterial drugs are not enough to solve the problem of bacterial infection. Another strategy currently used to study antibacterial is the antivirulence strategy, that is, to directly inhibit the expression of bacteria-related virulence factors without inhibiting the growth of bacteria, and reduce or lose...

Claims

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Application Information

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IPC IPC(8): A61K36/60A61P31/04A61K127/00
CPCA61K36/60A61K2236/33A61K2236/333A61K2236/35A61K2236/39A61K2236/51A61K2236/55
Inventor 朱虎孙世伟李慧刘奥周万龙戴晓芸卜祥国
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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