Method for resistance identification of semi-wild cotton on secondary salinization in seedling stage for water culture

Abstract

The invention relates to the field of stress-resistant morphology and physiology of plants, in particular to a method for resistance identification of semi-wild cotton on secondary salinization in a seedling stage. The method comprises the following steps of preparation of seeds; sprouting of seeds; seedling transplanting, and water culture; forced treatment; trait investigation; resistance evaluation. The method has the advantages that the resistance of semi-wild cotton on secondary salinization in the seedling stage is verified in room for the first time, and the identification is free from season limits and outside climate influence; the identification cycle is short, the salt-resistant identification period in the field cotton seedling stage is about 50d, and the identification period of water culture in room is merely about 25d.

Description

Technical field [0001] The invention relates to the field of plant stress resistance morphology and physiology, and specifically relates to a method for identifying secondary saline-alkali resistance at the seedling stage of semi-wild cotton. Background technique [0002] Semi-wild cotton (semi-wild cotton) is a race of upland cotton, native to the Mexican highlands of Central America and the Caribbean. Semi-wild cotton is rich in genetic diversity and has many phenotypes that conventional upland cotton cultivars do not possess under adversity stress. [0003] In the research on cotton seedling salt resistance identification, due to (1) long field identification period, limited by the growth environment, limited survey characteristics and susceptibility to climatic conditions, the identification results are unstable and the identification can only be completed in a specific season; 2) In indoor identification, whether sand culture or hydroponic identification methods are used, onl...

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Problems solved by technology

[0003] In the research on salt resistance identification of cotton seedlings, due to (1) long field identification period, limited by the growth environment, limited investigation of traits, and easy to be affected by climatic conditions, the identification results are unstable and the identification can only be completed in a specific season; ( 2) Indoor identification, regardless of whether sand cultivation or hydroponic identification methods are used, usually only a single salt stress (NaCl) is considered, and the complex salt composition and associated high alkali content of saline-alkali soil in actual production are often ignored. For example, in Xinjiang, which is known as the world's saline-alkali soil museum in China, the area of ​​saline-alkali soil is large, the weight of accumulated salt and the composition of ions are complex, and the salt-resistant materials screened out in single-salt identification will not be able to be used in practice; (3) at present The indicators selected for the identification of cotton seedling salt tolerance are various, including phenotype-related indicators, physiological and biochemical indicators, etc. The determination of some indicators not only prolongs the time of salt-tolerant identification and increases the identification cost, but also due to various factors Influenced by large measurement errors, it is difficult to confirm the accuracy of the results, and what is more, whether the indicators can be used for salt tolerance identification remains to be verified

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