Method for authenticating drought resistance of cotton seeds

A cotton seed and drought-resistant technology, applied in the direction of germination equipment, etc., can solve the problems of difficult control of the identification technology environment, long identification period, large human error, etc., and achieve the effects of short identification period, easy promotion and implementation, and easy operation

Inactive Publication Date: 2019-02-01
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for identifying the drought resistance of cotton seeds, which aims to solve the shortcomings of traditional identification technology that the environment is not easy to control, the identification period is long, the efficiency is low, and the shortcomings of large human errors

Method used

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  • Method for authenticating drought resistance of cotton seeds
  • Method for authenticating drought resistance of cotton seeds
  • Method for authenticating drought resistance of cotton seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1. Sample preparation:

[0041] From the well-mixed net seeds, randomly select 2400 cotton seeds from China Cotton Research Institute 41.

[0042] Step 2. Stress culture: Five concentration gradients of 10%, 15%, 20%, 25% and 30% were set, and each concentration was repeated 4 times, with 100 grains per repetition. Add 20 ml of polyethylene glycol solution of 10%, 15%, 20%, 25% and 30% to each 100 g of dry sand, pH is 6.0-7.5, stir evenly, and spread wet sand with a thickness of 1.5 cm in the germination box. Arrange 4 repeated seeds to be tested on the sand bed evenly, smooth the seeds with a flat-bottomed utensil, make half of them sink into the sand, cover them with a layer of wet sand with a thickness of 1.0cm, and spread them evenly. , cover, and put it in a light incubator at 30 °C ± 1 °C. After the cotyledons are exposed to the sand surface, start to light for 8 hours during the day, and the light intensity is ≥ 1200lx.

[0043] Step 3. Control culture: 4 ...

Embodiment 2

[0057] Step 1. Sample preparation:

[0058] From the well-mixed net seeds, randomly select 800 cotton seeds each of H177, China Cotton Research Institute 49, China Cotton Research Institute 79 and Xinluzao 72. Among them, Zhong H177 is a drought-resistant control variety, and the other three are main planting varieties in the field.

[0059] Step 2. Stress culture: 4 repetitions, 100 capsules per repetition. Add 20 ml of 15% polyethylene glycol solution to each 100 g of dry sand, the pH is 6.0 to 7.5, stir evenly, spread wet sand with a thickness of 1.5 cm in the germination box, and place 4 repeated seeds to be tested. Arrange it on a flat sand bed, use a flat-bottomed container to smooth out the seeds, make half of them sink into the sand, cover with a layer of wet sand with a thickness of 1.0 cm, spread evenly, cover, and place in 30 ℃ ± 1 ℃ In the light incubator, after the cotyledons emerged from the sand surface, the light for 8 h during the day was started.

[0060] ...

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Abstract

The invention belongs to the technical field of seed screening and assessment in the field of agriculture and discloses a method for authenticating drought resistance of cotton seeds. The method includes: randomly counting full cotton seeds from completely-mixed pure seeds, dividing the seeds into a polyethylene glycol stress treatment group and a contrast treatment group, cultivating in an illumination incubator for preset time, researching germinating seed amount of each treatment group, calculating drought resistance coefficients, and authenticating and assessing drought resistance of the cotton seeds through analytic comparison of the drought resistance coefficients. The method is low in cost, short in period, easy to operate, convenient to popularize and implement everywhere and especially suitable for large-scale genetic resource drought resistance screening.

Description

technical field [0001] The invention relates to a method for identifying seeds, in particular to a method for identifying drought resistance of cotton seeds, and belongs to the technical field of seed screening and evaluation in the agricultural field. Background technique [0002] Drought is a worldwide problem. According to statistics, global arid and semi-arid arable land accounts for 42.9% of the world's total arable land, and my country's arid and semi-arid areas account for 48% of the country's total arable land. The loss of crops caused by drought stress ranks first among all abiotic stresses. Cotton is the most important natural fiber crop and the most important economic crop in the world. It is not only the main raw material of the textile industry, but also the raw material of national defense, medicine, chemical industry and other industrial sectors. [0003] However, as the area of ​​cultivated land in my country is shrinking, the conflict between grain and cott...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02
CPCA01C1/025
Inventor 王延琴陆许可马磊匡猛金云倩王俊娟周大云方丹徐双娇王爽荣梦杰蔡忠民黄龙雨吴玉珍
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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