Method for rapidly identifying and screening anti-blight banana seedling

Abstract

The invention discloses a method for rapidly identifying and screening anti-blight banana seedling. The method comprises the steps of establishment of banana seedling hydroponic system, inoculum preparation and inoculation, daily management of banana seedling, disease situation investigation and grading. Banana seedling stage inoculation and hydroponic identification method can effectively control interference due to differences in soil structure, nutrition, microorganism, etc., during banana seedling stage inoculation identification process, rapidly and accurately identify anti-blight bananaseedling, continuously identify large amount of tissue-cultured banana seedlings in small area, perform multiple times of identification in one year, improve test result reliability, shorten identification period, and save land occupation and test cost.

Description

technical field [0001] The invention relates to the technical field of agricultural plant protection, in particular to a method for rapidly identifying and screening banana fusarium wilt disease-resistant seedlings. Background technique [0002] Banana Fusarium wilt is an important disease distributed in major banana producing areas in the world. As banana Fusarium wilt is a typical soil-borne disease, its control is very difficult, and the application of disease-resistant varieties to control banana Fusarium wilt is currently the most economical and effective method. [0003] At present, the identification of banana fusarium wilt-resistant seedlings commonly used in my country generally adopts the method of pot plant inoculation with soil, fine sand or substrate as the carrier, such as the most widely used pot plant root irrigation method and root soaking method, etc., the above-mentioned The potted plant inoculation method mainly has the following problems: 1. Since the in...

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Problems solved by technology

[0003] At present, the identification of banana fusarium wilt-resistant seedlings commonly used in my country generally adopts the method of pot plant inoculation with soil, fine sand or substrate as the carrier, such as the most widely used pot plant root irrigation method and root soaking method, etc., the above-mentioned The potted plant inoculation method mainly has the following problems: 1. Since the interaction of soil microorganisms will affect the accuracy of the test results, large-scale soil disinfection must be carried out in advance, which is inconvenient to operate and is not conducive to a large number of screening of disease-resistant seedlings; 2. The identification period It usually takes more than 30 days for one identification; 3. The contact between seedlings and soil may be disturbed by other soil-borne diseases, which will affect the accuracy of test results

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