Method for quickly identifying tobacco variety resistance to bacterial wilt

A technology for bacterial wilt and tobacco, applied in the field of plant disease resistance, can solve the problems of reducing the breeding efficiency of varieties, increasing the cost of resource evaluation, and spending production seasons, so as to simplify the field resistance identification procedure, ensure consistency and reliability. Repetitive, distraction-reducing effects

Inactive Publication Date: 2016-05-11
中国烟草总公司福建省公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic tobacco areas have been using the traditional field artificial disease nursery root irrigation method to identify varieties of bacterial wilt resistance. Although this method is effective in the screening and evaluation of bacterial wilt resistance resources, it requires a long period of identification. It takes the entire production season, which will undoubtedly increase the cost of resource evaluation and reduce the efficiency of breed selection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The method is as follows: select 10 sterile tobacco seedlings grown on 1 / 2 MS medium to the 5th leaf stage and have similar plant sizes, remove the MS medium attached around the clean root system in the ultra-clean workbench, and use scissors After root injury, soak the root of the plant in the bacterial suspension for 30 minutes, blot the excess bacterial solution on the surface of the root with sterilized filter paper, replant it in MS medium, and immerse the root in sterilized water as a control, 30°C, light intensity 2600lx, 16h Culture under photoperiod conditions, observe and record the disease condition every other day, and calculate the disease index according to the severity of the disease.

[0050] The severity of the condition: the severity of the condition is divided into 5 grades, and the standards are as follows:

[0051] Grade 0 - asymptomatic;

[0052] Grade 1 - 1 leaf wilting;

[0053] Grade 2 - 2 to 3 leaves wilt;

[0054] Grade 3 - 4 leaves wilting...

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PUM

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Abstract

The invention relates to a method for quickly identifying tobacco variety resistance to bacterial wilt. The method comprises the following steps: respectively selecting 10 sterile tobacco seedlings which grow to be at a 4-6-leaf-age stage on a 1/2 MS culture medium and are nearly consistent in plant size, removing the MS culture medium attached onto the peripheries of clean root systems in a super-clean workbench, soaking plant roots in bacterial suspension liquid for 30 minutes after the roots are cut by scissors, absorbing up the redundant bacterial suspension liquid on the surfaces of the roots by using a piece of sterile filter paper, newly planting in the MS culture medium, soaking the roots in sterile water as a control, culturing for 16 hours at the temperature of 30 DEG C and under the photoperiod condition that the light illuminance is 26001x, observing and recording disease conditions every other day and calculating disease indexes according to the disease severity.

Description

technical field [0001] The invention belongs to the field of plant disease resistance, and in particular relates to a method for quickly identifying resistance to bacterial wilt of tobacco varieties. Background technique [0002] At present, the identification methods of bacterial wilt resistance of traditional tobacco varieties include: net room inoculation, artificial disease nursery and field natural disease nursery method. These methods have complicated identification procedures and require a long period of identification. Usually, only one identification can be completed every year or the entire growth period, and there are urgent improvements in the following aspects. From the perspective of economic feasibility: When evaluating the resistance of germplasm resources in large quantities, it takes a lot of manpower, material resources and financial resources; From the perspective of stability and reliability of results: easily affected by factors such as climate, soil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01H4/00
CPCA01G7/00A01H4/008
Inventor 顾钢徐进冯洁陈顺辉许景升张昊周挺
Owner 中国烟草总公司福建省公司
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