Authenticating method of bemisia tabaci resistance of cigarette variety

An identification method and technology for Bemisia tabaci are applied in the field of identification of tobacco varieties against Bemisia tabaci, which can solve the problems that identification results are easily affected by the environment, difficulty in Bemisia tabaci resistance, and identification difficulties, and reduce workload. And the effect of identification work intensity, short identification period and saving test time

Inactive Publication Date: 2017-11-17
TOBACCO RES INST CHIN AGRI SCI ACAD +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The insect is small and the adults are extremely mobile. It is very difficult to identify the resistance of tobacco to whitefly
Through the investigation of 216 tobacco variety resources infected with whitefly nymphs in field experiments, an identification method for tobacco resistance to whitefly was initially proposed. Although the difference in resistance to whitefly among tobacco varieties can be compared, the identification results are easily affected by the environment. impact, the scope of application also has certain limitations
Due to the late occurrence time of whitefly and the difficulty of identification, there are no other research reports on the identification of tobacco resistance to whitefly at home and abroad.

Method used

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  • Authenticating method of bemisia tabaci resistance of cigarette variety
  • Authenticating method of bemisia tabaci resistance of cigarette variety
  • Authenticating method of bemisia tabaci resistance of cigarette variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The distribution law of embodiment 1 Bemisia tabaci on tobacco

[0036] (1) Test material:

[0037] The tested insect, Bemisia tabaci, was collected from tobacco fields in Zhucheng, Shandong in 2012. It was identified as Q-type Bemisia tabaci by mtDNACO I gene sequence sequencing method (Luo Chen et al., 2002), and Q-type Bemisia tabaci has been included in the world's 100 species One of the most dangerous invasive organisms. The white cauliflower bean was used as the host for subculture in the greenhouse, and every 3 generations of continuous breeding were checked to ensure the purity. At the beginning of the experiment, the progeny population had been fed continuously for 12 generations. The Bemisia tabaci population was reared in an insect culture greenhouse under the conditions of (26±1)°C, RH60%~80%, 14L︰10D. When the whitefly grows to an adult, the whitefly is inoculated on the host white flower bean, and the whitefly is inoculated using a conventional inoculat...

Embodiment 2

[0065] Example 2 Different Tobacco Varieties in Greenhouses Differences in Resistance to Bemisia Tabaci

[0066] According to the practice of identifying crop resistance to diseases and insect pests, it is divided into five grades: high resistance, resistance, intermediate (resistance), sensitivity, and high sensitivity. The average number of nymphs and adults infected with whitefly on a single leaf of tobacco is used as the resistance evaluation index. According to the difference in resistance, the resistance of tobacco to whitefly is divided into 5 grades, and the resistance to whitefly is evaluated according to the insect population ratio of each tobacco variety. The insect quantity ratio (ф)0≦ф<0.20 If there are 30 species, they are high-resistance varieties (HR); if the insect population ratio (ф) 0.20≦ф<0.40, 38 are resistant varieties (R); if the insect population ratio (ф) is 0.40≦ф<0.60, there are 15 species , which is the moderately resistant variety (M); there are 1...

Embodiment 3

[0095] Example 3: Differences in the resistance of different tobacco varieties to whitefly in the field

[0096] (1) Test materials: the tobacco varieties for testing are the same as the 134 kinds of tobacco varieties in the above-mentioned embodiment 1 and embodiment 2, and the tobacco seedling cultivation adopts strict industrialized seedling cultivation methods. The environmental conditions of the seedling greenhouse are 22-26°C, RH60%-80%, 14L︰10D.

[0097] (2) Test method:

[0098] year 2013. The field experiment was completed in the experimental field of Tobacco Research Institute, Chinese Academy of Agricultural Sciences. The tested tobacco varieties that will be cultivated are transplanted into a plot (with an area of ​​100m2) according to each variety. 2 ), with 3 replicates each. No pesticides were used, and other management was carried out according to the conventional production of tobacco leaves. When the planted tobacco is in the period of vigorous growth to...

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Abstract

The invention discloses an authenticating method of bemisia tabaci resistance of a cigarette variety. The authenticating method comprises the steps of a, planting a tobacco variety to be authorized, a resistance contrast tobacco variety and a host used for breeding bemisia tabaci; b, cultivating a bemisia tabaci population, and inoculating the bemisia tabaci swarm to the host to conduct host subculture raising; c, when the tobacco variety to be authorized and the resistance contrast tobacco variety grow to the same larva old, releasing bemisia tabaci to the tobacco variety to be authorized and the resistance contrast tobacco variety; d, counting and recording the adult insect quantity and the nymph quantity of random one of the fifth leaf to the eighth leaf of the tobacco variety to be authorized and the resistance contrast tobacco variety from top to bottom respectively; e, calculating the insect quantity ratio; f, conducting resistance evaluation. The authenticating method of the bemisia tabaci resistance of the cigarette variety has the advantages of being capable of effectively reducing the workload, improving the accuracy, authenticating a single tobacco variety or a small quantity of tobacco varieties, and making the authorization condition controllable.

Description

technical field [0001] The invention belongs to the technical field of crop resistance breeding, in particular to a method for identifying the resistance of tobacco varieties to whitefly. Background technique [0002] Bemisia tabaci belongs to the family Bemiptera of Homoptera, which originally occurred in tropical, subtropical and temperate marginal areas. In the second half of the 20th century, it spread rapidly to all parts of the world with the transfer of interregional seedlings and other trade activities. It is an important species. worldwide economic pest. Nicotiana belongs to Solanaceae, Solanaceae annual or limited perennial herb with slightly lignified base. Inflorescence terminal, conical, many flowers; capsule ovate or oblong, about equal to persistent calyx, flowering and fruiting in summer and autumn. [0003] In recent years, bemisia tabaci damage occurs frequently in my country's tobacco fields, which has a great impact on the production and quality of toba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01G7/06
CPCA01G7/00A01G7/06
Inventor 陈德鑫任广伟曾庆宾张淼张瑞萍李斌刘海兵李茂业王秀芳王新伟闫芳芳
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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