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Method for identifying resistance of wheat take-all disease

A technology for wheat take-all disease and resistance identification, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of subjective influence on identification results, heavy identification workload, long time, etc., and achieve identification The effect of short cycle, easy control of conditions and high accuracy

Inactive Publication Date: 2014-03-12
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] A simple, fast and accurate identification method for wheat take-all disease resistance is provided to solve a series of problems such as heavy workload, long time, and subjective influence on the identification results of wheat take-all disease at present

Method used

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  • Method for identifying resistance of wheat take-all disease
  • Method for identifying resistance of wheat take-all disease
  • Method for identifying resistance of wheat take-all disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Determination of take-all disease resistance of Jingzhou rye and Panmai No. 5

[0021] The resistance identification method is as follows:

[0022] A) Preparation of the plate of pathogen of take-all wheat

[0023] Cultivate the strong pathogenic strain G1037 of the pathogen of wheat take-all (this strain has been publicly published by the unit) on a PDA plate at 25°C for 7-15 days. At this time, the mycelium has covered the plate. Perforate the edges to form a fungus dish.

[0024] B) Preparation of material to be inoculated

[0025] The seeds of Jingzhou rye, Panmai No. 5 and Yangmai 158 were soaked in 1% NaClO solution for 2 minutes, and then rinsed with sterile water for 3 times; two layers of wet filter paper were placed in a 9 cm petri dish, and the filter paper was fully absorbent but not Sow the above-mentioned sterilized seeds evenly on the filter paper with the ventral groove facing down, 30 grains per dish, and culture at 25°C for 2 days; select ...

Embodiment 2

[0034] Example 2 Identification of take-all disease resistance of 51 wheat varieties

[0035] Using the method of Example 1, taking Yangmai 158 as a control variety, the resistance of 51 wheat varieties to take-all was identified. In order to test the reliability of this method, these varieties were identified twice at different times.

[0036] The resistance standard of wheat take-all disease is 5 grades, immune (I), severity=0; resistant (R), 0

[0037] The results of 2 resistance identifications of table 151 wheat varieties

[0038]

[0039]

[0040]

[0041] The results of the two identifications are relatively consistent, and the correlation coefficient is 0.8192, indicating that this identification method is

[0042] Good repeatability.

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Abstract

The invention relates to a method for identifying the resistance of the wheat take-all disease. The method comprises the following steps of adopting a disease-susceptiblevariety of yangmai 158 as a control variety, carrying out identification of the resistance of the wheat take-all disease on the wheat to be measured, i.e., disinfecting seeds of the wheat to be measured and the yangmai 158, and putting the seeds in a culture dish for pregermination; selecting and moving a seedling with three germinated seed roots and the root length being 1.5-2.5cm into the culture dish padded with water-absorbing filtering paper; grafting the middle part of each seed root into a bacterial disk with strongly-pathogenic bacterial strain with gaeumannomyces graminis var. tritici, culturing for five days, measuring the length of lesionson the wheat to be measured and the yangmai 158, and calculating the severity of the wheat take-all disease of the wheat to be measured by comparison with the yangmai 158. The method has the advantages of short identification period, simple and convenient operation, small occupied space and high accuracy.

Description

technical field [0001] The invention relates to a plant disease resistance identification method, in particular to a wheat take-all disease resistance identification method, which belongs to the technical field of plant protection. Background technique [0002] Wheat take-all (Take-all) is a typical root soil-borne disease, mainly caused by wheat variety Gaeumannomyces graminis var. Tritici (Ggt for short). The mycelium of the pathogen invades the root of the wheat plant and multiplies in large numbers, destroying the root tissue cells and blocking the root ducts, which prevents the normal transportation of nutrients and water in the plant, resulting in reduced tillering of the wheat plant, yellowing, dwarfing and even death of the plant. When the wheat enters the mature stage , white spike symptoms appear. The pathogen can infect wheat during the whole growth period, and the symptoms are most obvious in the adult plant stage. In moist soil, the ectophytic hyphae of the pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00
Inventor 邓渊钰陈怀谷李伟孙海燕张爱香郑青松
Owner JIANGSU ACAD OF AGRI SCI
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