Method for quickly inducing spore production of stemphylium botryosum

A rapid technology for sporulation induced by plant pathogenic bacteria, which can solve the problems of waste of manpower, energy and precious time of scientific researchers, failure to ensure that all strains produce sporulation, and poor stability of the medium, etc., to achieve shortening The effect of identification cycle, improving sporulation efficiency, and short sporulation time

Active Publication Date: 2016-07-13
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are some reports on the spore-forming medium of Puccinia sporogenes, mainly including PCA, V8 medium, PDA medium, MEA medium, PSA medium, oat medium, TWA-W medium, etc., but the above-mentioned culture medium was found to be Poor base stability, special requirements for bacterial strains, can not guarantee that each strain can produce sporulation; and, even if it can produce sporulation, it takes a lot of time, the average sporulation time is more than 20 days, which greatly wastes manpower, energy and scientific research worker's precious time

Method used

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  • Method for quickly inducing spore production of stemphylium botryosum
  • Method for quickly inducing spore production of stemphylium botryosum
  • Method for quickly inducing spore production of stemphylium botryosum

Examples

Experimental program
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Effect test

Embodiment 1

[0071] Embodiment 1: rapid induction of sporulation of stalk mold

[0072] The implementation steps of this embodiment are as follows:

[0073] A. Plate culture

[0074] 3 rings are preserved on the PDA solid growth medium slant that is made up of 20 weight parts potatoes and 2 weight parts glucose Puccinia tomato FQ14092805 is inoculated on the PDA plate culture medium, by Ningbo Jiangnan Instrument Factory under trade name " Ningbo instrument " , in a constant temperature light incubator sold under the model "GXZ light incubator", cultivated at a temperature of 25°C for 5 days according to a 12-hour light and 12-hour dark photoperiod, so that the mycelia are covered with a petri dish; the stalk mold is Puccinia tomato and Puccinia solani.

[0075] B. Cultivation of tomato seedlings

[0076] Hongza No. 10 tomato seeds were wrapped with clean gauze, soaked in water at a temperature of 55°C for 15 minutes, and then transferred to room temperature water for 24 hours. "The ge...

Embodiment 2

[0080] Embodiment 2: rapid induction of sporulation of stalk mold

[0081] The implementation mode of this embodiment is carried out according to the implementation mode of Example 1, just get 1 ring and preserve the Puccinia solani FQ140618115 on the slope of the PDA solid medium; Zhongza No. 105 tomato seeds are soaked at a constant temperature for 20min; The sliced ​​tomato leaves were cultured under conditions of temperature 25°C, relative humidity 70%, and 12-hour light and 12-hour dark photoperiod, observed under a microscope and calculated the number of conidia formed, and the results are listed in Table 2.

Embodiment 3

[0082] Embodiment 3: rapid induction of sporulation of stalk mold

[0083] The implementation mode of this embodiment is carried out according to the implementation mode of embodiment 1, just gets 2 rings and is preserved on the PDA solid growth medium slope Puccinia solani FQ14091004; The sliced ​​tomato leaves were cultured under the conditions of temperature 25°C, relative humidity 60% and 12 hours of light and 12 hours of dark photoperiod, observed under a microscope and calculated the number of conidia formed, the results are listed in Table 2.

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Abstract

The invention relates to a method for quickly inducing spore production of stemphylium botryosum. The method comprises the following steps: plate cultivation, cultivation of tomato seedlings and spore production induction. The method disclosed by the invention is used for quick and simple induction of spore production of the stemphylium botryosum, so that the spore production time can be greatly shortened, and the authentication period can be shortened; human and material resources and precious time of scientific research workers are effectively saved; the method has great application potential and higher economic value.

Description

【Technical field】 [0001] The invention belongs to the technical field of sporulation induced by plant pathogenic bacteria. More specifically, the present invention relates to a method for rapidly inducing sporulation of Puccinia spp. 【Background technique】 [0002] Stemphylium can infect a variety of crops worldwide, and may cause a variety of vegetable diseases, such as tomato, lettuce, pepper, cabbage, spinach, garlic, and tomato is particularly serious. The United States, Israel, New Zealand, etc. have all reported tomato leaf spot caused by Puccinia spp., which has caused huge economic losses to local tomato production. In recent years, tomato gray leaf spot has been particularly serious in 500,000 mu in Northeast my country, North China, East China, and South China. Among them, Shouguang in Shandong, Haikou in Hainan, and Tianyang in Guangxi are the most severely affected, with a total area of ​​more than 100,000 mu and a loss of more than one thousand. ten thousand yu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/645
CPCC12N3/00
Inventor 谢学文李宝聚石延霞柴阿丽
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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