Cabbage type rape high oleic acid QTL and molecular marker closely linked thereto

A technology of Brassica napus and molecular markers, which is applied to the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as unclear genetic basis, improve accuracy and selection efficiency, and solve genetic resources. The effect of a single, accelerated improvement process

Active Publication Date: 2018-07-03
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The gene controlling oleic acid content reported in production is basically the FAD2...

Method used

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  • Cabbage type rape high oleic acid QTL and molecular marker closely linked thereto
  • Cabbage type rape high oleic acid QTL and molecular marker closely linked thereto
  • Cabbage type rape high oleic acid QTL and molecular marker closely linked thereto

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Using the DH population to locate novel QTLs for oleic acid

[0033] 1. Construction and field experiment of DH localization population of Brassica napus

[0034] The Brassica napus strain ZP1 (with oleic acid content of 67.68%, Brassica napus ZP1, Brassica napusL.ZP1) was delivered to China. Wuhan. Wuhan University Chinese Type Culture Collection Center on November 10, 2017 for preservation, and the deposit number is CCTCC NO: P201722) was used as the female parent, and the Brassica napus strain A254 was used as the male parent (the content of oleic acid was 72.296%, patent number ZL 2009102734352; publication number CN101824472A) to obtain F1, and F1 was planted. Culture (refer to the literature for microspore culture method, Yu Fengqun, etc., Research on some culture factors to improve the seedling rate of Brassica napus microspore embryoid, Journal of Crops, 1997, 23(2): 165-168) obtained from 150 strains Lines composed of double haploid (referred to as ...

Embodiment 2

[0041] Example 2: Validation of a novel QTL for oleic acid using the F2 population

[0042] 1. F2 group construction

[0043] In order to further confirm the above results, the applicant selected the two parents ZP1 without A5 locus as the female parent and the conventional variety Huashuang 5 (a new national cabbage rape variety approved by the National Crop Variety Approval Committee of the Ministry of Agriculture of China in 2004). (Certification number: Guoshenyou 2004006), which has been widely promoted and planted in China), F1 was obtained by crossbreeding, and F2 population was obtained through F1 selfing, and the effect and stability of OLEA9 were verified by F2 population. In a subpopulation of F2 containing 170 individuals, the distribution of oleic acid content was bimodal, and analysis of this marker near the peak indicated significant phenotypic differences among the three different genotypes.

[0044] 2. Parental resequencing of F2 population and development of...

Embodiment 3

[0047] Example 3: Application of QTL site-specific marker for oleic acid content of Brassica napus A9 chromosome

[0048] The marker BnA129 (see Table 2 for nucleotide sequence) near the peak was selected from the developed InDel markers for effect testing. The marker primer amplified the target fragment of 100 bp in the genomic DNA of the high oleic acid parent ZP1, and amplified the target fragment of 109 bp in the genomic DNA of the low oleic acid content parent Huashuang 5 (see figure 2 ), there is a 9bp InDel difference between the two, so the amplified fragment can be used as a co-dominant molecular marker for the trait of high oleic acid content in Brassica napus.

[0049] The genotypes of 170 F2 plants were analyzed by BnA129, of which 42 plants were A genotype with the same pattern as the parent ZP1, carrying the A9 chromosome high oleic acid locus allele, and the average oleic acid content was 67.47±1.76%; 51 individual plants were B genotype and had the same bandi...

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Abstract

The invention belongs to the technical field of preparation of rapeseed molecular markers and particularly relates to cabbage type rape high oleic acid QTL and a molecular marker closely linked to theQTL. Through screening, a novel molecular marker BnA129 closely linked to oleic acid content QTL is obtained and has a nucleotide sequence shown in the formulas of SEQ ID NO: 3 and/or 4. A primer BnA129 of a detection molecular marker OLEA9 is constructed and has a nucleotide sequence shown in the formulas of SEQ ID NO: 1 and 2 in the sequence table. The quantitative trait locus OLEA9 and molecular markers can be used in molecular marker-assisted selection in the improvement of high oleic acid content characters of cabbage type rape and fine mapping and map cloning of oleic acid character sites.

Description

technical field [0001] The invention belongs to the technical field of rapeseed molecular marker screening, in particular to the high oleic acid QTL of Brassica napus and a molecular marker closely linked thereto. The oleic acid QTL of the present invention and the molecular markers closely linked thereto can be used for improving the oleic acidity of Brassica napus, as well as in the fine mapping and map-based cloning of the oleic acid locus. Background technique [0002] Rapeseed is one of the most important oil crops in the world. The edible and processing characteristics and quality of vegetable oil mainly depend on the fatty acid composition of seeds. The main fatty acids in canola include three unsaturated fatty acids, namely oleic acid, linoleic acid and linolenic acid. The ratio of these three fatty acids has a great influence on the quality of rapeseed oil. Oleic acid is an eighteen carbon monounsaturated fatty acid. High-oleic varieties are less prone to oxidati...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 周永明赵青吴建蔡光勤杨庆勇
Owner HUAZHONG AGRI UNIV
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