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Methods and compositions to select cotton plants resistant to cotton root knot nematode

a technology of cotton root knot and composition, which is applied in the field of plant breeding and disease resistance, can solve the problems of limited commercial cultivars with rkn resistance, and achieve the effects of high oil production, high protein production, and increased yield

Inactive Publication Date: 2011-07-14
MONSANTO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0009]The present invention also provides for an elite cotton plant produced by: a) providing a population of cotton plants; b) genotyping at least one cotton plant in the population with respect to a cotton genomic nucleic acid marker selected from the group comprising SEQ ID NOs: 1-38; and c) selecting from the population at least one cotton plant comprising at least one allele associated with RKN resistance. The elite cotton plant of the present invention can exhibit a transgenic trait. The transgenic trait is selected from the group consisting of herbicide tolerance, increased yield, insect control, fungal disease resistance, virus resistance, nematode resistance, bacterial disease resistance, mycoplasma disease resistance, modified oils production, high oil production, high protein production, germination and / or seedling growth control, enhanced animal and human nutrition, low raffinose, environmental stress resistance, increased digestibility, improved processing traits, improved flavor, nitrogen fixation, hybrid seed production, and reduced allergenicity. The herbicide tolerance can be selected from the group consisting of glyphosate, dicamba, glufosinate, sulfonylurea, bromoxynil, 2,4, Dichlorophenoxyacetic acid, and norflurazon herbicides.

Problems solved by technology

However, commercial cultivars with RKN resistance are limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Phenotypic Rating Scale

[0163]To asses the reaction of plants to RKN, cotton plants were grown in growth chambers and artificially inoculated with 2,500 nematode eggs approximately 7 days after emergence. Plant roots were examined for galling 45-50 days after inoculation. A galling index of 0 (no galls) to 5 (100% roots with galls) was used to rate the plants. Table 1 provides the phenotypic rating scale used to identify RKN reaction in cotton plants.

TABLE 1Phenotypic Rating Scale Used for RKN ReactionRatingPhenotypic Description0No visible galls; healthy root system11-2 galls; healthy root system23-12 galls; small gall size313-30 galls; large size galls more visible on tap root431-60 galls; severe galling with large gall size5>60 galls; severe galling with >75% roots with largegalls; root system non-functional

example 2

Identification of SNP Markers Associated with RKN Resistance

[0164]A mapping population was developed from the cross of the RKN resistant parent M240 with RKN susceptible parent 33B. A total of 250 near-isogenic lines (NILs) were developed for the mapping population. Ten replicates of each line were evaluated for reaction to RKN as described in Example 1.

[0165]Eleven SNP markers located on Chromosome A11 were used to screen the NIL mapping population. Of these, SNP marker NG0204877 was found to be highly associated with RKN resistance. Of 248 lines screened, the mean galling index of lines with the TT genotype was significantly lower than those with the AA genotype. Table 2 provides the mean galling index for lines with the genotype AA, AT, and TT. A t-test analysis was performed and the p-value for no mean difference between AA and TT genotypes on the galling index was 3.84×10−82. The marker NG0204877 is on Chromosome A11 at position 181.1.

TABLE 2Marker NG0204877 (SEQ ID NO. 31) is ...

example 3

Use of SNP markers for Monitoring RKN Resistance

[0166]Additional SNP markers are located on Chromosome A11. Table 3 provides the marker names, chromosome position, and the position of the polymorphism in the marker, and alleles.

[0167]In a breeding program, one or more markers provided in Table 3 can be used to select for and to introgress RKN resistance into a cotton plant. A cotton breeder can select one or more markers which are polymorphic between parents in a breeding cross to select progeny with the genotype of the RKN resistant parent.

TABLE 3SNP Markers on Chromosome A11 for Detecting RKN Resistance.SEQ IDChromosomeSNPMarkerNO:PositionPosition1Allele 1Allele 2NG02042121142.5303ACNG02048652143367AGNG02033543145253AGNG02079594145.9333ATNG02044755147382AGNG02108926150.7323CTNG02064477150.7447AGNG02098298150.7149ACNG02106289150.7171AGNG020355010152.2253GTNG020412911158.4325CGNG020931412159.2272ATNG020993613159.862AGNG020783814160.1385AGNG020901215160.159ATNG020991416160.1520ATNG02...

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Abstract

The present invention is in the field of plant breeding and disease resistance. More specifically, the invention provides a method for breeding cotton plants containing one or more quantitative trait loci that are associated with resistance to Root Knot Nematode(RKN), a disease associated with Meloidogyne incognita. The invention further provides germplasm and the use of germplasm containing quantitative trait loci (QTL) conferring disease resistance for introgression into elite germplasm in a breeding program, thus producing novel elite germplasm comprising one or more RKN resistance QTL.

Description

CROSS-REFERENCES TO RELATED APPLICATIONS[0001]This application claims the benefit under 35 U.S.C. §119(e) of U.S. Provisional Application No. 61 / 092,649 filed on 28 Aug. 2008. The entirety of the application is hereby incorporated by reference.INCORPORATION OF SEQUENCE LISTING[0002]A sequence listing containing the file named “pa—53664.txt” which is 70.5 kilobytes (measured in MS-Windows®) and created on Aug. 25, 2009, comprises 103 nucleotide sequences, and is herein incorporated by reference in its entirety.FIELD OF INVENTION[0003]The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding cotton plants containing quantitative trait loci that are associated with resistance to cotton root knot nematode (RKN), a disease associated with Meloidogyne incognita (Kofoid and White) Chitwood. The invention further includes germplasm and the use of germplasm containing quantitative trait loci (QTL) conferrin...

Claims

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Application Information

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IPC IPC(8): A01H5/00A01H1/02A01H1/04C07H21/04C12Q1/68A01H5/10
CPCA01H1/04C12Q1/6895A01H5/10A01H6/604A01H1/045
Inventor BHATTI, MUHAMMADCANTRELL, ROY G.XIAO, JINHUA
Owner MONSANTO TECH LLC
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