Extraction and purification method for determining organophosphorus pesticide residues in fruits and vegetables
A technology for organophosphorus pesticides, vegetables and fruits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of increasing method application steps, increasing environmental pollution, and increasing detection labor, so as to reduce dosage, improve detection efficiency, reduce Detection steps and human effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] An extraction and purification method for the determination of organophosphorus pesticide residues in fruits and vegetables, taking bayberry as an example, such as figure 1 As shown, it consists of the following steps in turn:
[0030] A. Accurately weigh 25g of ground fruit and vegetable samples in a 100ml centrifuge tube;
[0031] B. Accurately add 50ml of acetonitrile extract;
[0032] C. Use a homogenizer to disperse and extract at a speed of 10kr / min for 1min, then add 5g of sodium chloride, cover and shake vigorously for 10s;
[0033] D. Stand still for 30 minutes, and purify after the stratification is clear;
[0034] E. Take 10ml of supernatant and purify it through MycoSep 226 AflaZon+ column;
[0035] F. Take 1ml of the purified acetonitrile extract in the sample bottle, and wait for the determination on the machine.
[0036] The acetonitrile extract purified by MycoSep 226 AflaZon+ purification cartridge is colorless and transparent, which can significant...
Embodiment 2
[0038] An extraction and purification method for the determination of organophosphorus pesticide residues in fruits and vegetables, adding dichlorvos, profenofos, acephate, acephate, phorate, and oxymethol with a mass concentration of 0.08mg / kg to bayberry, cucumber, and pepper Fruit, diazinon, monocrotophos, dimethoate, dimethophos, parathion, fenthion, isofenfos, isocarbophos, quinalphos, methion, ethion, 19 kinds of organophosphorus pesticides such as thionon, the spiked recovery spectra obtained under the following chromatographic conditions, the results are as follows: Figures 2 to 4 shown. In the prior art, the chromatogram of the 10th injection of the cucumber sample that was not purified by the MycoSep 226 AflaZon+ purification column was spiked and recovered, and the type and concentration of the added pesticides were consistent with those of the present invention. Such as Figure 5 shown. And comparing the result spectrogram of the present invention with the prio...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com