The invention relates to an enclosed animal experiment operation box and aims to solve the problems that the conventional pharmacological animal experiment causes environmental pollution which causes damage to instruments and equipment and influences experimenters' health, the operation view is not clear, and the light is insufficient. The enclosed animal experiment operation box comprises a box body and an operational table assembled inside the box body and used for fixing an experimental animal, and is characterized in that the box body comprises a base, a frame, a front baffle, a back baffle, a left baffle, a right baffle and an erector, fixed limiting posts and adjustable limiting posts are arranged on the operational table and limited to the rails of the base, guiding rods and an adjusting screw rod used for controlling the erector to move are arranged on the base, and a magnifier is arranged in the picture frame of the erector. According to the invention, the enclosed animal experiment operation box has the advantages of being simple in operation, convenient to adjust and the like, and provides modern experimental equipment with a clean and excellent working environment; the view is clean and the light is sufficient when experiments are carried out; the accuracy and the success rate of the experiments are significantly improved, and the experimental appropriation expenditure is reduced.