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81results about How to "Reduced amplification" patented technology

Enclosed animal experiment operation box

InactiveCN104257439AAvoid contamination and damage to equipmentProtect physical and mental healthAnimal fetteringAnimal scienceEngineering
The invention relates to an enclosed animal experiment operation box and aims to solve the problems that the conventional pharmacological animal experiment causes environmental pollution which causes damage to instruments and equipment and influences experimenters' health, the operation view is not clear, and the light is insufficient. The enclosed animal experiment operation box comprises a box body and an operational table assembled inside the box body and used for fixing an experimental animal, and is characterized in that the box body comprises a base, a frame, a front baffle, a back baffle, a left baffle, a right baffle and an erector, fixed limiting posts and adjustable limiting posts are arranged on the operational table and limited to the rails of the base, guiding rods and an adjusting screw rod used for controlling the erector to move are arranged on the base, and a magnifier is arranged in the picture frame of the erector. According to the invention, the enclosed animal experiment operation box has the advantages of being simple in operation, convenient to adjust and the like, and provides modern experimental equipment with a clean and excellent working environment; the view is clean and the light is sufficient when experiments are carried out; the accuracy and the success rate of the experiments are significantly improved, and the experimental appropriation expenditure is reduced.
Owner:SHENYANG MEDICAL COLLEGE

Voltage sag detecting method suitable for dynamic voltage restorer

The invention belongs to the field of electric system state monitoring, and particularly relates to a voltage sag detecting method suitable for a dynamic voltage restorer. The method includes the steps of establishing phase shifting components through the three selected detection points which include the front detection point, the middle detection point and the rear detection point and are arranged at equal intervals, obtaining a d-axis DC component and a q-axis DC component of a fundamental wave through a Butterworth low-pass filter after alpha beta-d q conversion is conducted on the detection points and a middle sampling point, conducting quadratic sum addition on the d-axis DC component and the q-axis DC component and calculating through a root-mean-square to obtain a voltage amplitude, conducting arc-tangent function operation on the quotient obtained by dividing the q-axis DC component by the d-axis DC component, and obtaining a phase jump angle through the difference between the phase detection value at the earlier moment and the phase detection value at the later moment. By means of the method, the certain white noise resistance is still achieved while the real-time requirement is met, and the method is quite suitable for detecting voltage sags in the dynamic voltage restorer.
Owner:NORTH CHINA ELECTRIC POWER UNIV (BAODING)

Kit and method for detecting COVID-19 virus by combining nested isothermal amplification with gene editing

The invention relates to the technical field of molecular biology, and discloses a kit and a method for detecting COVID-19 virus by combining nested isothermal amplification with gene editing. The kitcomprises: (1) a nested isothermal amplification system: adopting a nested amplification mode, wherein an outer primer pair is used for a first round of isothermal amplification, an inner primer pairis used for a second round of isothermal amplification, a product of the first round of isothermal amplification can be used as a template of the second round of isothermal amplification, and the inner primer pair is used for isothermal amplification; (2) a nucleic acid detection system based on gene editing: using a Cas13a protease and a specific probe to identify a nested RAA enriched templatewith a target sequence; and (3) a visual detection system: utilizing a transverse flow test strip to quickly judge whether virus molecules exist or not. The kit is simple and convenient to operate, does not need complex temperature control equipment, is rapid and efficient, can interpret a result in only one hour, has extremely high amplification efficiency, can detect five copied virus molecules,and is low in pollution risk.
Owner:安徽微分基因科技有限公司

Methods for Amplification of Nucleic Acids on Solid Support

The present invention provides methods for amplifying a nucleic acid from a sample containing a mixture of nucleic acids utilizing a solid support. Methods are provided utilizing user-defined primer oligonucleotides for directional amplification that assists in further manipulation of the target nucleic acid, such as sequencing. Methods are also provided utilizing blocker and displacer oligonucleotides for generating amplified target nucleic acids of defined length. One of these methods provides a first oligonucleotide and a second oligonucleotide affixed to a solid support or separate solid supports. The first oligonucleotide is blocked to prevent extension from the 3′-terminus and has a sequence complementary to a first portion of a target nucleic acid. The second oligonucleotide has a sequence that is identical to a second portion of the target nucleic acid. In this method, a sample is applied to the solid support and the target nucleic acid within the sample binds said first oligonucleotide. The solid support is then washed to remove unbound nucleic acids. A primer sequence containing a target binding region and a polymerase promoter sequence is then annealed to the bound target nucleic acid and extended producing a first duplex nucleic acid. The target sequence is then removed leaving a first nucleic acid that can now bind the second oligonucleotide. The second oligonucleotide is extended to produce a second duplex nucleic acid that contains a second nucleic acid. The second nucleic acid is then amplified by adding a polymerase.
Owner:AEGEA BIOTECH
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