504 results about "Primer (molecular biology)" patented technology

The invention provides a primer probe set for detecting a novel corona virus SARS-CoV-2 and a detection method, and belongs to the technical field of molecular biology detection. The primer probe setfor detecting the novel corona virus SARS-CoV-2 by an RT-RAA fluorescence method comprises an upstream primer, a downstream primer and a probe, wherein the nucleotide sequence of the upstream primer is as shown in SEQID NO.1, the nucleotide sequence of the downstream primer is as shown in SEQID NO.2, and the probe is a substance, wherein the 31st-site basic group of a nucleotide sequence as shownin SEQID NO.3 is modified with a fluorescence report base group, the 32nd-site basic group of the nucleotide sequence as shown in SEQID NO.3 is replaced with a tetrahydrofuran residue, and the 34th-site basic group of the nucleotide sequence nucleotide sequence as shown in SEQID NO.3 is modified with a quenching base group. The primer probe set provided by the invention is quick, accurate and acute in detection, and simple and convenient to operate, the specificity can reach 100%, and detection of the novel corona virus SARS-CoV-2 can be completed within 30min.

The invention discloses a method for detecting gene mutation genotyping based on a Taqman-ARMS (Amplification Refractory Mutation System) technology, relating to the field of molecular biology. According to the method, an ARMS mutation enrichment technology and a Taqman-MGB (Minor Groove Binder) specificity fluorescence detection technology are combined, a mutation target sequence is subjected to specificity PCR (Polymerase Chain Reaction) amplification by using an ARMS primer, a Taqman-MGB probe is used for carrying out specificity locus detection on an amplification product, and specific mutation is identified on the basis of Real-time PCR. Compared with mutation detection technologies such as direct sequencing, chip detection and the like, the method has the advantages of strong specificity, high sensitivity, simplicity and rapidness in operation, high flux and the like when used for detecting gene mutation.

The invention discloses a probe, a primer, a kit, and a detection method for detecting novel coronavirus SARS-CoV-2, and application, belongs to the technical field of molecular biology nucleic acid detection technology, and aims to solve problems in field detection, reduce pollution caused in detection process, increase virus RNA detection sensitivity and specificity. The invention provides the probe and the primer for detecting novel coronavirus SARS-CoV-2, and the kit containing the probe and the primer. The invention further provides a whole-course encapsulated procedure for exponential amplification from RNA (WEPEAR method). According to the invention, the detection sensitivity on N gene as low as 1ag (about 4 copies) is realized; the detection sensitivity on S gene as low as 1 copy is achieved, meanwhile, double genes of virus nucleic acid RNA based on RT-RPA (or RT-ERA) are detected at the same time so as to further improve the reliability of the detection result, and the kit can be used for field detection or household detection.

The invention relates to genetic engineering and molecular biology, and particularly discloses a plant anther specific promoter PCHF15 and application thereof. The invention further provides an expression vector with the plant anther specific promoter PCHF15, a genetic expression box and a carrier with the genetic expression box. The invention also provides a primer pair for amplifying the plant anther specific promoter PCHF15. The plant anther specific promoter PCHF15 is an endogenous gene of rice, is beneficial to genetic engineering of the rice, and drives exogenous genes to accurately express specificity in pollen, so that a novel method for driving specificity expression of the exogenous genes in the pollen is provided.

The invention discloses a method for quickly identifying Heliothis armigera and Helicoverpa assulta, and belongs to the field of molecular biology. The method comprises the following steps of: extracting total DNA by adopting a standard phenol-chloroform protocol or a DNA extraction kit; amplifying a front sequence of mitochondria CO1 by using a DNA barcode primer, and using the amplified sequence as a mark; and performing diagnostic characters of nucleotides. By the method, different biotypes and larvae or incomplete individuals of the same variety of Heliothis armigera and Helicoverpa assulta can be identified; and the method has the characteristics of quickness and convenience, and is more accurate and reliable than the traditional morphologic identification.

The invention discloses a PCR (Polymerase Chain Reaction) amplification primer for quickly detecting klebsiella pneumonia and application thereof, and belongs to the field of animal bacteriology and molecular biology. The PCR amplification primer is prepared from an upstream primer having a nucleotide sequence as shown in SEQ ID NO:1 and a downstream primer having a nucleotide sequence as shown inSEQ ID NO:2; the PCR amplification primer is used for preparing a PCR amplification kit for detecting the klebsiella pneumonia. A PCR detecting method provided by the PCR amplification kit has high specificity and sensitivity, good repeatability and high credibility and is capable of specifically detecting the klebsiella pneumonia and quickly and accurately obtaining a detecting result; meanwhile, the PCR amplification kit is low in cost and simple in operation, is suitable for being used at the primary level and can be used as a quick, accurate and simple detecting tool for quick laboratoryidentification of the klebsiella pneumonia and large-scale epidemiological investigation.

The invention belongs to the field of plant molecular biology, and in particular relates to a method for identifying male or female of the siraidia grosvenorii by means of PCR and provides specific DNA segment sequences of male plants of the siraidia grosvenorii, the design of PCR primers, the optimization of PCR conditions and the like.

The invention relates to the field of molecular biology, and in particular to a galectin-3 binding protein, and preparation and application thereof. The galectin-3 binding protein is shown as an amino acid sequence SEQ ID No.1 in the sequence table. The preparation method is as below: using Cynoglossus semilaevis cDNA as a template; conducting PCR amplification by primers F1 and R1; connecting PCR products to an expression vector to obtain a plasmid pETG3BP; conversing BL21DE3 to obtain a transformant BL21/pETG3BP; inducting by isopropyl-beta-D-thiogalactoside; and purifying the recombinant protein with affinity chromatography column to obtain the galectin-3 binding protein. The galectin-3 binding protein has significant binding ability to various bacteria.

The invention relates to the technical field of molecular biology, and particularly discloses a sarcoma fusion gene joint detection primer group, kit and detection method. The kit provided by the invention comprises: 1) a special linker for DNA library construction; 2) a specific composite primer for detecting fusion genes and point mutation; and 3) a composite primer for library amplification. DNA and RNA of a to-be-detected sample are respectively extracted, reverse transcription is performed on RNA to synthesize cDNA, the cDNA is mixed with DNA, fragmentation, terminal repair and linker ligation are performed on the mixture, PCR amplification is performed on the product, a library is enriched, and high-throughput sequencing is performed. According to the fusion gene detection kit and detection method provided by the invention, various fusion genes or mutations related to sarcoma can be detected at one time, the detection efficiency is improved, the operation is convenient, the consumption time is short, and the sensitivity is relatively high and can reach 0.1%.

The invention belongs to the field of molecular biology (tumor screening), particularly relates to a technology for carrying out early warning and screening on liver tumor, and aims to solve the technical problem to provide a new choice for early prediction of the liver tumor. The technical scheme of the invention is that a kit for carrying out early warning and screening on liver tumor comprises a primer which is used for amplifying the following genes of an RASSF1A (Ras-association Domain Family 1A) gene of which a promoter is in a CpG island methylation state, a p16 gene, ELF (Embryonic Liver Fodrin), an SOCS (Suppressors of Cytokine Signaling)-3 gene, an SFRP1 (Secreted Frizzled-Related Protein 1) gene and a genome repetitive sequence LINE1 (Long Interspersed Nuclear Elements 1). According to the kit disclosed by the invention, a new effective inspection method is provided for carrying out early warning and screening on early liver tumor and liver cirrhosis; the cost is low, the use is very convenient, the flexibility is high, the specificity is good, and the application prospect is very good.

The invention discloses a nucleic acid composition, kit and detection method for detecting methylation of lung cancer related genes, and relates to the field of molecular biology. The nucleic acid composition comprises a primer composition, wherein the primer composition comprises a first primer pair for detecting methylation of an RASSF1A gene, a second primer pair for detecting methylation of anSHOX2 gene and a third primer pair for detecting methylation of a PTGER4 gene. The nucleic acid composition can realize simultaneous detection of methylation of the RASSF1A, HOX2 and PTER4 genes on the basis of a fluorescent quantitative PCR platform, and has the characteristics of high sensitivity and high specificity.

The invention relates to a haemophilus parasuis detection kit and a detection method thereof, and belongs to the technical field of molecular biology. The detection kit comprises a primer pair, PCR Mix, a position control and dd H2O. The haemophilus parasuis detection kit disclosed by the invention has the primer pair designed according to an mviN gene sequence in a high conserved domain, is good in specificity, and can accurately distinguish the haemophilus parasuis strain LC from haemophilus paragallinarum, actinobacillus pleuropneumoniae, pasteurella muhocida, arcanobacterium pyogenes, staphylococcus aureus and streptococcus suis; the detection kit and the detection method provided by the invention are high in sensitivity, short in consumed time, accurate in detection, and important in significance of monitoring haemophilus parasuis reproduction, disease occurrence and prevalence as well as timely control of the disease.

The invention discloses an SSR (Simple Sequence repeats) primer group based on Fraxinus Velutina Torr. transcriptome sequencing information development and an application of the primer group in germplasm identification, belonging to the technical field of molecular biology. The SSR primer group comprises 42 pairs of primers, wherein the nucleotide sequences of the first pair to the forty-second pair of primers are shown in SEQ ID No.1 to SEQ ID No.84. Experiments verify that the SSR primer group disclosed by the invention has the advantages of rich polymorphism, good repeatability, clear electrophoretic band and the like. The SSR primer group can be effectively used for researches on germplasm identification, DNA fingerprint establishment and the like. A germplasm identification method is sensitive and reliable, can be used for rapidly and accurately realizing and finishing distinguishment and identification of Fraxinus chinensis germplasm, and has important significances in identification and intellectual property protection of Fraxinus chinensis germplasm resources and genetic breeding of genetic breeding molecules.

The invention relates to primers and a kit for detecting a molecular marker linked to major QTL for controlling corn stalk strength, a detection method for detecting corn stalk strength, and applications of the primers, the kit and the detection method, and belongs to the technical field of corn genetic breeding and molecular biology. According to the present invention, a recombinant selfing linepopulation containing 241 families is constructed through Zheng 58*D863F, and genetic linkage map analysis is performed, such that four QTL for controlling corn stalk strength are detected, wherein the high stalk strength major QTL qRPR1.07 is identified simultaneously at the position 1.07 of the chromosome 1 of the corn from Hainan Ledong and Henan Yuanyang Demonstration Base, and is positioned between two SSR markers bnlg1556 and umc2232 so as to explain nearly 20% of genetic variation; and the molecular marker linked to the major QTL for controlling corn stalk strength can directionally andprecisely screen corn materials with high stalk strength at the early stage of growth so as to save the breeding cost and the breeding time, and is used for lodging-resistant breeding of corn.

The invention provides a wheat TaARF12 gene and application thereof, and belongs to the field of molecular biology of crops. A designed primer clones a coding region sequence of TaARF12 from cDNA of Chinese spring young ears, and the coding region sequence of the TaARF12 gene in homologous chromosomes A, B and D is represented as SEQ ID NO. 1-3 respectively. A TaARF12-specific RNAi fragment is selected, an RNAi vector is created, and Fierder is transformed. A CRISPR/Cas9 vector is used to create a gene editing vector containing two target sites and transform wild-type Fierder. Obtained RANi transgenic lines and gene editing lines have similar phenotypes, with plants becoming shorter and ears becoming longer. It is indicated that the wheat TaARF12 gene can regulate the plant height and theear length, and the plants become shorter and the ears become longer after the gene is interfered and expressed.

The invention discloses a real-time fluorescent quantitative PCR (polymerase chain reaction) detecting reagent kit for an orf virus and belongs to the technical field of viral molecular biology detection. The real-time fluorescent quantitative PCR detecting reagent kit for the orf virus comprises a pair of primers of nucleotide sequences shown in SEQ ID NO:1-2 and a TaqMan probe of a nucleotide sequence shown in SEQ ID NO:3. The detecting reagent kit can quickly and accurately detect the orf virus from samples and is high in detecting sensitivity and simple and convenient to sample, detecting efficiency is greatly improved, pollution can be effectively prevented, and the detecting reagent kit is of practical significance in the field of inspection and quarantine of import and export animals.

The invention discloses primers and a method for quickly distinguishing orange varieties, and belongs to the field of molecular marking in molecular biology. The method comprises the steps of: designing RAPD (Random Amplified Polymorphic Deoxyribonucleic Acid) random primers according to the gene sequences of the orange, and screening to obtain 16 random primers; extracting DNA (Deoxyribonucleic Acid) of a plurality of orange varieties, and diluting for later use; and amplifying the DNA of the unknown orange varieties by utilizing the random primers one by one, distinguishing out the variety with unique characteristic band, and building a tree-shaped identification figure. According to the invention, 48 orange varieties can be distinguished on a molecular level through a plurality of PCR (Polymerase Chain Reaction), the practicability of RAPD molecular marking utilizing 11 base primers in plant variety identification is embodied to a certain extent, and the obtained tree-shaped identification figure is more illustrative than the clustering tree, namely the primers for distinguishing any two varieties can be found out according to the variety identification figure; and the method can realize early identification of orange seedlings, and has wide universality in other species.

The invention discloses a nucleic acid chromatography detection kit of Candida albicans, a detection method and an application thereof, belonging to a field of molecular biology and immunology. According to the invention, polymerase chain reaction of molecular biology and immunochromatography are combined, thereby realizing rapid detection of the Candida albicans. The kit comprises PCR amplification and test strip detection, wherein primers are subjected to specific modifications, corresponding coating objects and tags are selected during preparation of the test strips, and a specific compound is formed during the reaction process, thereby realizing combination of the molecular biology technology and the immunochromatography technology, and realizing detection of specific amplification products of the Candida albicans. Compared with traditional biochemical method, the method greatly saves detection time, simplifies operation steps, and achieves purposes of rapid, high-efficiency and accurat detection and screening. The kit plays an important effect on clinic detection and screening process of the Candida albicans.