Internal standard gene suitable for detecting transgene carnation exogenous gene, preparation method and application thereof

A technology of internal standard gene and exogenous gene, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and applications
CN101705304AInactive Publication Date: 2010-05-12SHANGHAI ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI ACAD OF AGRI SCI
Publication Date
2010-05-12
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention relates to an internal standard gene suitable for detecting a transgene carnation exogenous gene. According to the three standards of the intraspecies non-specificity, the interspecies specificity and the single-copy or low-copy number of the internal standard gene and by the analysis of biologic informatics and the experimental verification of molecular biology, the invention searches out an ANS gene conforming to the standards to be used as the internal standard gene for the qualitative and quantitative PCR detection of transgene carnation in the carnation. A qualitative PCR primer is ANS-F1 / ANS-R1, a quantitative PCR primer is ANS-F2 / ANS-R2, and a quantitative PCR probe is ANS-probe. The ANS gene is used as the internal standard gene for the qualitative and quantitative PCR detection and the copy number analysis of the transgene carnation.
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Description

technical field

[0001] The invention relates to an internal standard gene, preparation method and application of carnations, in particular to an internal standard gene suitable for qualitative and quantitative detection and copy number detection of exogenous genes in transgenic carnations, belonging to the technical field of bioengineering. Background technique

[0002] The so-called internal standard gene is a type of gene in a certain plant that has the characteristics of interspecies specificity, intraspecific non-specificity, and low copy number. Interspecies specificity means that selected genes are specific to this species, but have very low homology in other species. Preliminary data can be obtained through the analysis of biological information in the network database, candidate genes can be selected, and then the candidate genes can be screened through experiments, and finally the appropriate inter-species specific genes can be selected as the standard genes in plan...

Claims

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