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Internal standard gene suitable for detecting transgene carnation exogenous gene, preparation method and application thereof

A technology of internal standard gene and exogenous gene, applied in botany equipment and methods, biochemical equipment and methods, applications, etc.

Inactive Publication Date: 2013-12-04
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the internal standard gene of carnation qualitative and quantitative detection in the world at present.

Method used

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  • Internal standard gene suitable for detecting transgene carnation exogenous gene, preparation method and application thereof
  • Internal standard gene suitable for detecting transgene carnation exogenous gene, preparation method and application thereof
  • Internal standard gene suitable for detecting transgene carnation exogenous gene, preparation method and application thereof

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Embodiment Construction

[0041] In order to make the technical means, creative features, goals and effects of the present invention easy to understand, the present invention will be further described below in conjunction with specific illustrations.

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Abstract

The invention relates to an internal standard gene suitable for detecting a transgene carnation exogenous gene. According to the three standards of the intraspecies non-specificity, the interspecies specificity and the single-copy or low-copy number of the internal standard gene and by the analysis of biologic informatics and the experimental verification of molecular biology, the invention searches out an ANS gene conforming to the standards to be used as the internal standard gene for the qualitative and quantitative PCR detection of transgene carnation in the carnation. A qualitative PCR primer is ANS-F1 / ANS-R1, a quantitative PCR primer is ANS-F2 / ANS-R2, and a quantitative PCR probe is ANS-probe. The ANS gene is used as the internal standard gene for the qualitative and quantitative PCR detection and the copy number analysis of the transgene carnation.

Description

technical field [0001] The invention relates to an internal standard gene, preparation method and application of carnations, in particular to an internal standard gene suitable for qualitative and quantitative detection and copy number detection of exogenous genes in transgenic carnations, belonging to the technical field of bioengineering. Background technique [0002] The so-called internal standard gene is a type of gene in a certain plant that has the characteristics of interspecies specificity, intraspecific non-specificity, and low copy number. Interspecies specificity means that selected genes are specific to this species, but have very low homology in other species. Preliminary data can be obtained through the analysis of biological information in the network database, candidate genes can be selected, and then the candidate genes can be screened through experiments, and finally the appropriate inter-species specific genes can be selected as the standard genes in plan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/29C12N15/10
Inventor 唐雪明朱宏李鹏陶世如蒋玲曦王金斌谭芙蓉吴潇赵凯宿学峰杨世方
Owner SHANGHAI ACAD OF AGRI SCI
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