Human STRtyper PCR amplification fluorescence detection reagent kit

Abstract

The invention discloses a human STRtyper PCR enlarged fluoroscopic examination reagent kit with quick speed detecting, accuracy, good repeatability, good versatility, low price and practicality. The applicability of the reagent kit for Chinese is remarkable in particular. The serial products of the invention comprise the three following kits: (1) a STRtyper-20G kit with the largest STR gene locus of the global and most powerful recognizing ability; (2) a STRtyper-16GC kit which is more suitably used to build a criminal DNA database of Chinese population and identify the identity; (3) a STRtyper-10F / G kit with brand new STR gene locus. Each kit product comprises the component of 1.1 ml * 1 tube of STR PCR reaction mixture; 0.55 ml * 1 tube of STRtyper primer mixture; 120 microlitre * 1 tube of HS-Taq DNA polymerase; 0.3ml * 1 tube of STR control DNA; 50 microlitre * 1 tube of STRtyper allelomerphic gene mixture; 0.5 ml * 1 tube of internal standard; 30 microlitre* 1 tube of 310 spectrum correcting standard; 30 microlitre * 1 tube of 3100 spectrum correcting standard.

Description

technical field [0001] The invention relates to a human gene detection kit, in particular to a human STRtyper PCR amplification fluorescence detection kit. Background technique [0002] Human Genomic DNA has 3×10 9 bp, 10% of which are tandem repeats, called satellite DNA. According to the length of the repeating unit, it can be divided into large satellites, medium satellites, small satellites and microsatellites. Among them, the repeat unit is only composed of 2 ~ 7bp called microsatellite, also known as short segment tandem repeat sequence (Short Tandem Repeat, referred to as STR). The number of repeating units of microsatellite DNA in different human genomes is variable, thus forming extremely complex allelic fragment length polymorphisms. According to this law, a new STR genotyping technology has been established in recent years, which can identify different individuals and carry out genetic analysis among individuals, so as to provide forensic medicine, archaeology,...

AI Technical Summary

Problems solved by technology

So far, only two American companies, PE and Promega, have successively launched STR genotype detection kits abroad, but this type of STR genotype detection kit is mainly designed for the STR loci of the Caucasian population. The applicability of the STR genotype detection of the yellow race is relatively poor, and the price is expensive. The use of imported reagents to establish the Chinese criminal DNA database is also susceptible to interference from external factors.