Rapid detection kit and detection method for breast cancer susceptibility genes

Abstract

The invention discloses a rapid detection kit for breast cancer susceptibility genes, which comprises polymorphic detection of 5 SNP (single nucleotide polymorphism) corresponding to each of Foxp3, FGFR2, CD14 and BRCA1 genes. The invention further discloses a rapid detection method for the breast cancer susceptibility genes. The rapid detection kit and detection method have the benefits as follows: by means of semi-nested AS-PCR (allele-specific polymerase chain reaction) amplification and AGE (agarose gel electrophoresis) detection, genetic typing is performed according to DNA (deoxyribonucleic acid) electrophoretic bands, individuals with potential breast cancer susceptibility are screened out, so that breast cancer is prevented; and the method is simple and convenient to operate, short in detection time, low in cost, high in sensitivity and specificity and broad in application prospect.

Description

Technical field: [0001] The invention relates to the technical field of biological gene detection, in particular to a rapid detection kit and detection method for breast cancer susceptibility genes. Background technique: [0002] Breast cancer (Breast cancer) is known as the female killer, and its incidence rate is second only to lung cancer. According to the Cancer Report (GLOBOCAN 2008) released by the International Cancer Research Center of the World Health Organization, it is estimated that about 1.38 million women suffer from breast cancer every year in the world, and about 540,000 breast cancer patients die. In 2008, the number of new cases of female breast cancer in China was about 169,000, and the standardized incidence rate of the world population was 21.6 / 100,000, ranking second among all female malignant tumors; the mortality rate was 5.7 / 100,000, ranking sixth among female cancer deaths bit. [0003] Early diagnosis and early detection, the cure of breast cance...

AI Technical Summary

Problems solved by technology

[0004] At present, there are traditional detection methods for breast cancer detection clinically, including X-ray diagnosis, ultrasound imaging, cytology and histological diagnosis, etc. Limitations; There are also some genetic detection methods, such as immunohistochemical technology, fluorescence in situ hybridization technology, fluorescent quantitative PCR technology, gene chip technology, etc.

Immunohistochemical technology can detect the protein expression of tumors and target cells, but cannot observe the type of mutation at the gene level. FISH can directly and accurately determine whether the target gene is amplified, but it is expensive and cumbersome

Gene chip technology can detect a large number of people, a large number of loci, and a short time, but it is only used for published gene loci, with low repeat accuracy and high price

Fluorescent quantitative PCR technology reagents are expensive and require sophisticated instruments, etc.

The shortcomings of these technologies have greatly limited the application of early diagnosis of breast cancer

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