Human BRCA1/ BRCA2 gene mutation detection kit

Abstract

The invention discloses a human BRCA1 / BRCA2 gene mutation detection kit, which comprises an amplification box for amplifying all exons of breast cancer susceptibility genes (BRCA1 / BRCA2) and at least10bp of DNA sequences at the upstream and downstream of each exon, an amplification product library building box, a library building connection specificity connector box, a detection kit outer package box and a magnetic bead box. The amplicon capture sequencing technology is used; the difficulty and the complexity of the conventional probe capture sequencing technology are greatly reduced; meanwhile, the technical cost is effectively reduced; the popularization and the use of clinical hereditary breast cancer and ovarian cancer screening detection are facilitated.

Description

technical field [0001] The invention relates to the fields of molecular biology and medicine, in particular to a human BRCA1 / BRCA2 gene mutation detection kit. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. Breast cancer occurs in breast epithelial tissue and is a systemic disease. One of its biological characteristics is that occult metastatic lesions can occur in the early stage, and the development of lesions often takes several years or longer. Breast cancer is found all over the world, and its incidence rate is higher in developed areas than in underdeveloped areas, and higher in cities than in rural areas. With the development of the economy, the incidence of breast cancer in my country has been increasing year by year. Millions of people are newly diagnosed with the disease every year in our country, women account for 99%, and men account for 1%. [0003] Breast cancer susceptibility genes (BRCA) are important tumo...

AI Technical Summary

Problems solved by technology

Many existing breast cancer detection kits can only detect a few "ancestor mutations", or use Sanger sequencing methods, which will not only miss multiple disease-causing mutations but also increase the cost of use and take a long time

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