57results about How to "Guaranteed catch" patented technology

The invention discloses a high-flux detection method of a circulating DNA liver cancer driver gene. According to the method, a mutant gene which is the most common of the existing liver cancer is selected and combined with a second-generation high-flux sequencing technology to more comprehensively detect a gene mutant condition of the liver cancer. Since a blood plasma sample of a patient is adopted, the method can greatly reduce the detection injury of a patient; secondly, the whole-exome detection is performed on 75 liver cancer genes, the method is more comprehensive compared to the detection method based on real-time PCR and first-generation Sanger sequencing technology; and since only 75 genes are detected, the sample can be detected in a greater sequencing depth, so that the detection precision can reach 0.1 percent. Therefore, a reference can be provided for more and better individual precision therapeutic schemes of the patient.

The invention provides a three-dimensional bionic nanometer material for capturing circulating tumor cells and a preparation method thereof.The material is composed of porous graphene, microballoon spheres and a silicon substrate, a three-dimensional array structure is formed through electrostatic adsorption and self-assembly, chemical modification is carried out on the surfaces of the graphene and the microballoon spheres, E-selectin protein molecules and ligands used for cell capturing are modified and fixed, and a bionic structure for enhancing cell recognition is achieved.The material can be integrated in a micro-fluidic chip, recognition and capturing of the chip for the circulating tumor cells is are enhanced, separation efficiency is improved, and the captured cells can be detected and analyzed conveniently; a fixed recognition ligand is changed, the material can also be used for effectively capturing different types of cells, and rare tumor cells can be recognized and captured from peripheral blood samples.

The invention discloses a rapid and accurate screening method of a hereditary breast cancer mutant gene capable of covering all exome regions of the latest hereditary breast cancer. The method disclosed by the invention is characterized in that 3 to 5 ml of blood can be extracted from the individual human body, 3 to 5 micro g gDNA is extracted from the blood and ultrasonically broken and amplifiedso as to establish a human whole-genome library, then a hereditary breast cancer mutant gene scanning kit of the invention is used for capturing relevant disease genes, then a new-generation sequencing instrument is used for performing the high-flux sequencing to analyze and find out the mutation information related to the genes, thereby achieving a purpose of screening the hereditary breast cancer mutant genes.

The invention relates to a correlation filtering tracking algorithm based on fusion features and a self-adaptive updating strategy. A tracking method based on correlation filtering is adopted, and a robust target model is constructed by using sub-channel fusion features to predict the central position of a target. For the problem that a target is severely shielded, a self-adaptive model updating mechanism is introduced to solve the problem that firstly, the reliability degree of a current response graph is judged according to a confidence coefficient threshold value, and the higher the reliability degree is, the lower the possibility that the target in the image is shielded is. Constructing a self-adaptive updating function on the basis, updating the tracking model according to the function, ensuring that the model is updated at a very low learning rate when the target is severely shielded, and introducing noise as little as possible. When the appearance of the target is clear and interference factors such as shielding deformation do not exist, the model is updated at a very high learning rate, and it is guaranteed that the model can capture the latest target characteristics. By means of the measures, a very robust tracking result can be obtained under different challenging scenes.

The invention discloses a deafness-related gene capture kit and an application thereof. The invention provides a capture probe. The capture probe is as shown in 1) or 2) which are described in the specification, wherein 1) the capture probe is composed of probes as shown in sequences 1 to 113 in a sequence table; 2) the capture probe is composed of derivatives of probes in 1); and the derivative of each probe is a probe which is obtained by substituting and/or deleting and/or adding one or more nucleotides to each probe in 1) and has the same function. According to the deafness-related gene capture kit provided by the invention, target region sequencing analysis is carried out on a whole exon group or a deafness-related genome (respectively accounting for 1% and 0.01% of the whole genome)of a hereditary deafness patient; and most pathogenic mutation information of the disease is captured.

The invention provides a method for suppressing the pulse modulation continuous wave interference of a notch filter assisted by a variable gain amplifier. The method comprises the following steps of:filtering input mixed signals by the notch filter; performing carrier wave stripping on filtered mixed signals, and respectively mixing the mixed signals with sinusoidal carrier wave replica signals and cosine carrier wave replica signals to obtain mixing results I

(t) and Q

(t); performing amplitude detection on the filtered mixed signals, and outputting a corresponding compensation coefficient Gain by the variable gain amplifier according to an amplitude detection result; compensating the mixing result I

(t) to obtain a compensated mixing result I<Pamp>(t); and evaluating and outputting the actual value A(t) of a filtered and compensated signal amplitude according to Q

(t) and I<Pamp>(t). According to the method for suppressing the pulse modulation continuous wave interference ofthe notch filter assisted by the variable gain amplifier, a traditional notch filter is assisted by the variable gain amplifier to achieve effective suppression for pulse modulation continuous wave interference, and can compensate for lost useful signals, which provides a basis for subsequent high-precision tracking and positioning.

The invention discloses a trawl net device and method. The trawl net device comprises a tubular inner net having upper and lower end openings and a tubular outer net having upper and lower end openings; the bottom of the tubular inner net is connected with the bottom of the tubular outer net through a circular net; the tubular inner net, the tubular outer net and the circular net are equal in meshsize; both the tubular inner net and the tubular outer net are provided with a plurality of support rings; a circular plate is arranged at the upper end of the tubular inner net; the lower surface ofthe circular plate is provided with a plurality of first bait boxes fully filled with bait; each first bait box is provided with a first sliding door that is provided with a first unlocking mechanism. The trawl net device ensures normal fishing and also ensures sustainable development of fishery resources.

The invention provides a space shape following capturing net, which comprises a capturing net body. A close-up mechanism is arranged on the periphery of the capturing net body, and an adaptive claw isarranged on a net body of the capturing net body; the adaptive claw can stretch out along with launching of the capturing net body; after the capturing net body collides with a space target, the adaptive claw embraces the space target in the capturing net body; and the capturing net body wraps the target and is closed up through the close-up mechanism to capture the target. It can be ensured thatthe target is completely captured through the large-area covering characteristic of the capturing net body, by combining with the self-locking function of the adaptive claw and through the close-up mechanism, the capturing net body is closed up, it is ensured that the capturing rope net body and the captured target can form a more stable composite, and non-cooperative targets are accurately, reliably and efficiently captured on rails.

The invention discloses a high-flux detection method of a circulating tumor DNA lung cancer driver gene. According to the method, a mutant gene which is the most common of the existing lung cancer isselected, and combined with a second generation high-flux sequencing technology to more comprehensively detect the gene mutation condition of the lung cancer. Since a blood plasma sample of a patientis collected, the method can greatly reduce the detection injury of the patient; secondly, since the whole-exome detection is performed on 22 lung cancer genes, so that the method is more comprehensive compared with the detection method based on real-time PCR and first-generation Sanger sequencing technology; and since only 22 genes are detected, the sample can be detected in a greater sequencingdepth, so that the detection precision can reach 0.1 percent. Therefore, a reference can be provided for more and better individual precision therapeutic schemes of the patient.

The invention discloses an earth sound event positioning method and an instability disaster early warning method thereof, an earth sound sensor, a monitoring system and a readable storage medium thereof, and the earth sound event positioning method considers the arrival time, time domain parameters, frequency spectrum information and waveform shape of an earth sound signal when a wave generated by an earth sound event source reaches any monitoring point, then the non-uniformity of a propagation medium is comprehensively reflected, and the inversion precision of earth sound event positioning is finally improved. According to the instability disaster early warning method, precursor information of disaster instability is comprehensively considered from different dimensions, and an earth sound instability disaster early warning means with applicability, timeliness and low failure rate is constructed according to the change trend of precursor factors; according to the earth sound sensor provided by the invention, integration of signal acquisition and processing is realized; the collected earth sound signals are subjected to micro signal enhancement, filtering fidelity and other processing, the anti-interference capability is effectively improved, and multi-band effective earth sound signals are perceived.

The invention discloses a method for detecting mycobacterium typing. The method selects the most common genome information of mycobacterium tuberculosis and non-tuberculous mycobacteria at present, and combines the second-generation high-throughput sequencing technology to more comprehensively detect and identify the mycobacterium strain typing situation. Compared with PCR-RFLP and PCR-direct sequencing methods and a DNA probe identification method, the second-generation high-throughput sequencing technology has large throughput, short sequencing time, high accuracy, low cost and rich information, and accurate detection and identification of strains in a short period of time. With the help of high-throughput detection technology, the method can provide clinicians with more detailed straininformation of mycobacteria infected by patients, help doctors to make more accurate treatment plans, and reduce the treatment course of diseases, cost and adverse reaction.

The invention provides an anti-interference radar material level meter which comprises a device body, an emitter and reception antennas. The device body includes a square casing, the emitter is fixedly connected to the front end of the device body, the two reception antennas are fixedly connected to the left and right sides of the device body respectively, each reception antenna includes a sector-shaped casing, the direction of the sector of the reception antenna is the same with that of the termination of the emitter, a high-frequency filter and a low-frequency filter are fixedly connected inthe reception antenna, the signal input end of each high-frequency filter is electrically connected with the corresponding reception antenna, the signal output end of the high-frequency filter is electrically connected with the signal input end of the corresponding low-frequency filter, and the signal output end of the low-frequency filter is electrically connected with an electronic device in the device body. The material level meter has the advantages including filtering electromagnetic wave signals emitted not by the emitter, avoiding interference, ensuring the accuracy of signal reception, and improving the measuring precision.

The invention discloses a high-throughput sequencing assay method for Epstein-Barr virus (EBV) integration assay. The method utilizes the genome information of the Epstein-Barr virus (EBV) in combination with the second-generation high-throughput sequencing technology to more comprehensively assay the condition of EBV infection of a patient, overcoming the defects of conventional assay methods, such as low accuracy, high false positive and poor repetitiveness. The adoption of gene sequencing can help increasing accuracy, and compared with the classical Sanger sequencing method mostly adopted at present, the assay method has the advantages of higher assay throughput, higher accuracy, lower cost, richer information and the like. With the help of the second-generation high-throughput sequencing technology, the assay method can carry out high-precision assay on the human infection of the Epstein-Barr virus, so that specific treatment can be carried out at the infection stage to reduce theharm of the disease on the human body, and thereby the occurrence of tumor is decreased.

The invention relates to a working fluid level tester for an oil production well. The tester comprises an infrasonic wave generating device, an echo receiving device, a display device and a control system. The sleeve depth, the sleeve height, the mining place temperature, the crude oil viscosity and the temperature in a sleeve of a pre-detected oil well are obtained, the factors are taken into consideration, and the mining grade of the pre-detected oil well is calculated. Meanwhile, the infrasonic wave frequency and the monitoring interval of the infrasonic wave generating device are adjustedin real time by adopting different standards according to the crude oil exploitation grade, the i monitored working fluid level height Bi and the height interval where the working fluid level height Bi is located, so that the situation that the oil well to be monitored is monitored in real time at the good infrasonic wave frequency, accurate and clear feedback data are obtained, the accuracy of a monitoring result is improved, meanwhile, the monitoring interval is automatically adjusted in real time, energy is saved, and the situation that the position of the working fluid level is captured in the whole monitoring process is guaranteed.

The invention discloses a sexual dysplasia-related gene capture kit and an application thereof. The sexual dysplasia-related gene capture kit carries out target region sequencing analysis on the wholeexon group or the sexual dysplasia-related genome (respectively accounting for 1% and 0.01% of the whole genome) of a patient with hereditary dysplasia, most pathogenic mutation information of the disease is captured, and the kit has the advantages of small required sample amount, low cost and high throughput, can detect many samples, and promotes discovery of new pathogenic variation of hereditary dysplasia. The sexual dysplasia-related gene mutation site selected by the invention covers common hot spot mutation and other unusual mutation sites of Chinese people. The high throughput of the kit makes sexual dysplasia-related genes in people comprehensively screened, so the kit is of great significance to improving population quality and benefiting the society.

The invention discloses a user connection unit sleep wake-up method and device and a computer readable storage medium, the user connection unit sleep wake-up method comprises the following steps: the running state of each module in a user connection unit is detected in real time, wherein the user connection unit at least comprises an MCU and a CPU; when the abnormal state is detected, the CPU is controlled to send an awakening instruction to the MCU; and if the MCU is still not awakened after the awakening instruction is sent for a preset number of times, the MCU is reset. By implementing the method and the device, the probability of occurrence of sleep wake-up faults is reduced, the user experience is improved, a good brand image is formed, and the burden of after-sales engineers is relieved.

The invention relates to a liquefaction prevention method of an offshore wind power jacket liquefaction prevention suction cylinder device. The suction cylinder device comprises a plurality of suction cylinder bodies arranged on the periphery of the lower end of a jacket, wherein a plurality of vertically distributed first pressure sensors are arranged on the outer side end faces of the suction cylinder bodies, a plurality of second pressure sensors are arranged at the lower end positions of the suction cylinder bodies, drainage holes communicating with a cavity are formed in the upper ends of the suction cylinder bodies, an extrusion assembly is arranged at the upper end position of the inner side of the cavity, a plurality of conduction pipes are arranged in the suction cylinder bodies, one ends of the conduction pipes are located at the upper end position of the inner side of the cavity, the other ends of the conduction pipes are located at the lower end positions of the suction cylinder bodies, and vacuum pumps are connected to the conduction pipes. The liquefaction prevention method has the following beneficial effects that efficient and rapid liquefaction prevention under the limit environment is achieved, and therefore the building stability of the jacket is guaranteed.

The invention discloses a high-throughput sequencing detection method for drug-resistant sites of Mycobacterium tuberculosis. The method uses the existing mutation database of drug-resistant Mycobacterium tuberculosis and combines the second-generation high-throughput sequencing technology to achieve a more comprehensive, effective and rapid Detecting resistance loci to anti-TB drugs in Mycobacterium tuberculosis. This method overcomes the limitation of the number of detected gene loci in other drug resistance locus detection methods, and the inability to distinguish silent mutations of important genes, which leads to the difficulty of false positive results. With the help of high-throughput detection technology, it can provide better Treatment of drug-resistant tuberculosis provides more comprehensive diagnostic information that can help improve efficacy and reduce the use of ineffective drugs.

The invention discloses a facial and identity card recognition integrated machine with indoor high-efficient recognition, and belongs to the field of verficaition devices. A technical scheme is as follow: that facial and identity card recognition integrated machine comprises a machine body, an ID card recognition area, a face camera and a height detector. The ID card recognition area is fixedly connected with the middle of the front end of the airframe, the face camera is rotatably connected with one side of the airframe, and the height detector is mounted on the other side of the airframe, wherein, a motor is fixedly connected with the machine body; and an output end of the motor is rotatably connected with the face camera; A height detector comprises a photoelectric sensor and a receiver, wherein that receive is in a square plate shape, the receiver is fixedly connected on the side of the body away from the face camera, the receiver is vertical in the length direction, a plurality ofphotoelectric sensor are fixedly connected on the receiver at intervals, and the length of the photoelectric sensors is the same as the width of the receiver; Single-chip microcomputer is connected with motor, photoelectric sensor and receiver at the top of the body, which has the advantages of facial recognition for people of different heights.