PCR primers and application for amplifying human breast cancer susceptibility genes brca1 and brca2 coding sequences

Abstract

The invention relates to a PCR primer and application of the coding sequences of human breast cancer susceptibility genes BRCA1 and BRCA2 based on NGS technology. The PCR primers of the human breast cancer susceptibility genes BRCA1 and BRCA2 coding sequences include at least one pair of capture primers, and the sequences of the forward primer and reverse primer of each pair of capture primers include specific sequences and are compatible with the described Linker sequence to which the 5' end of the specific sequence is ligated. The PCR primers and applications for amplifying the coding sequences of human breast cancer susceptibility genes BRCA1 and BRCA2 based on NGS technology, the experimental operation involved in the whole method is simple, only involves PCR reagents and primer combinations, the cost is low, and double tags are introduced at the same time Sequence adapter sequence is used to distinguish different samples, which can realize high-throughput sample sequencing detection, and can effectively provide genetic detection support for risk assessment of human breast cancer and other BRCA susceptibility genes-related hereditary tumors or targeted drugs for BRCA mutations .

Description

technical field [0001] The invention relates to the field of medical molecular biology, in particular to a PCR primer for amplifying human breast cancer susceptibility genes BRCA1 and BRCA2 coding sequences and its application. Background technique [0002] Breast cancer is the most common cancer in women. According to the "2012 China Cancer Registry Annual Report" data, there are about 220,000 new breast cancer cases in my country every year, accounting for 15% of the world's new breast cancer cases (1.5 million). Among all malignant tumors in women, the incidence rate of breast cancer is as high as 16.8%, about 43 cases per 100,000 people, and the age of onset is younger and the incidence rate is on the rise. The incidence rate rises rapidly at the age of 25 and peaks at the age of 50. Therefore, the high incidence of breast cancer has seriously threatened the health of women in my country. [0003] Familial hereditary breast cancer accounts for 10% to 30% of all breast...

AI Technical Summary

Problems solved by technology

In terms of detection sensitivity, Sanger sequencing can generally only detect mutations with a mutation rate of more than 20%. For the detection of all coding sequences of BRCA1 and BRCA2, the detection throughput is relatively low, the cycle is long, and the cost of single-sample detection is also high.

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