High-density array type cell slide culture apparatus, storage apparatus and experimental apparatus

Abstract

The present invention discloses one kind of high density array type cell culturing device, storing device and experiment device. The cell culturing device includes carrier plate with ordered arranged slots of 0.3-0.4 mm depth and glass slides inside the slots. The storing device has one holder with ordered arranged openings for the circular sheets to be set in, and the openings have length smaller than the diameter of the circular glass slide, end width of 0.15-0.18 mm and middle width greater than the end width. The experiment device has experiment plate with ordered arranged notches for the sheets to be set in, and the notches have cross section shape matching the glass slides and depth of 4-6 mm. The present invention has the advantages of convenient use, high efficiency, high identity, etc.

Description

Technical field: [0001] The invention relates to a cell culture device, in particular to a high-density array type cell culture device, a storage device and an experiment device which are convenient to use, high in efficiency and good in identity. Background technique: [0002] In vitro cell culture simplifies environmental factors, eliminates the influence of some complex interference factors in vivo experiments, and is conducive to the application of various physical, chemical and biological and other external processing factors to explore and reveal the law of cell activities; in vitro cell culture is convenient for the use of various techniques and methods , analyze and observe the morphological and functional changes and internal mechanisms of cells under normal and changed living conditions; Many ethical problems are avoided. Therefore, in vitro cell culture has become one of the most commonly used experimental methods indispensable in various research fields of life ...

AI Technical Summary

Problems solved by technology

[0004] 1. The coverslips are easy to move and overlap on the bottom of the smooth bottle. Some coverslips grow cells on both sides due to gaps caused by overlapping, so that the growth conditions and areas of cells on each coverslip are different. There is a problem of deviation in the experimental results due to the poor identity of the slides; in order to avoid this problem, the experimenters usually arrange the coverslips loosely, and as a result, the number of cell slides prepared each time is very limited, generally only 7-10 It is difficult to meet the needs of multi-level and multi-index research;

[0005] 2. There is no special storage device for climbing slices. When the climbing slices are stored at -20°C for backup, it not only takes up a lot of freezing space and causes energy waste, but also takes a lot of trouble to take the climbing slices every time. damage the slide;

[0006] 3. There is no dedicated experimental device for climbing slices, and each experiment is to place the climbing slices directly on the bottom of the vessel, and use the vessel as a container to add a medicinal solution that can submerge the climbing slices. The demand for medicinal liquid is large; at the same time, due to the The surface area is large, and the liquid medicine is easy to evaporate during the experiment, which not only wastes the liquid medicine, but also makes it difficult to obtain accurate experimental results; every time a different liquid medicine is changed, the climbing piece needs to be taken out to dry the upper and lower surfaces, and the experimental operation steps are very cumbersome and inefficient

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