High-density array type cell slide culture apparatus, storage apparatus and experimental apparatus

A culture device and array-type technology, which is applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of large demand for liquid medicine, cumbersome operation steps, and multiple freezing spaces, and achieve Simple operation, saving freezing space and high cultivation efficiency

A culture device and array-type technology, which is applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of large demand for liquid medicine, cumbersome operation steps, and multiple freezing spaces, and achieve Simple operation, saving freezing space and high cultivation efficiency

CN1928063AActive Publication Date: 2007-03-14李宏 +5
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  • High-density array type cell slide culture apparatus, storage apparatus and experimental apparatus
  • High-density array type cell slide culture apparatus, storage apparatus and experimental apparatus
  • High-density array type cell slide culture apparatus, storage apparatus and experimental apparatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 As shown in Figures 1 and 2:

[0032] There is a circular slide plate 1 which is 2 mm smaller than the diameter of the culture vessel, and the slide plate 1 is made of autoclavable silica gel, metal or other materials. Both sides of the upper surface of the loading plate 1 are provided with handles 4 with a height of 0.5-0.8 to facilitate the taking and placing of the loading plate 1 . On the carrier plate 1, there are ordered downward concave circular grooves 2 arranged in an orderly manner. The depth of the circular grooves 2 is 0.3-0.4mm. The circular grooves 2 are equipped with circular slides that match the bottom of the grooves 2 3. The glass slide 3 is a standard glass slide with a thickness of 0.14-0.17 mm, and the right side wall of the tank 2 is provided with a slice-taking gap 5 . In order to distinguish the front and back of the slides, marks can be made on the slides, such as the letter "R" on the front of the slides with grinding (blasting) s...

Embodiment 2

[0045] Embodiment 2 is as shown in Figures 3 and 4:

[0046] Others are the same as in Embodiment 1, except that the groove 2 is surrounded by a silica gel plate and the protruding ring 10 on it, and the chip-taking notch 5 is arranged on the protruding ring 10 .

Embodiment 3

[0047] Example 3 is shown in Figure 5: the others are the same as in Example 1, except that groove 2 has a round shape and a large square shape. In the same experimental system, in addition to obtaining round cell slides, large square slides can also be obtained. For square slides, collect cells or their lysates in centrifuge tubes according to conventional physical (such as cell scraping) or chemical (such as cell lysis) methods, and then enter the procedure of DNA, RNA or protein sample preparation.

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Abstract

The present invention discloses one kind of high density array type cell culturing device, storing device and experiment device. The cell culturing device includes carrier plate with ordered arranged slots of 0.3-0.4 mm depth and glass slides inside the slots. The storing device has one holder with ordered arranged openings for the circular sheets to be set in, and the openings have length smaller than the diameter of the circular glass slide, end width of 0.15-0.18 mm and middle width greater than the end width. The experiment device has experiment plate with ordered arranged notches for the sheets to be set in, and the notches have cross section shape matching the glass slides and depth of 4-6 mm. The present invention has the advantages of convenient use, high efficiency, high identity, etc.

Description

Technical field: [0001] The invention relates to a cell culture device, in particular to a high-density array type cell culture device, a storage device and an experiment device which are convenient to use, high in efficiency and good in identity. Background technique: [0002] In vitro cell culture simplifies environmental factors, eliminates the influence of some complex interference factors in vivo experiments, and is conducive to the application of various physical, chemical and biological and other external processing factors to explore and reveal the law of cell activities; in vitro cell culture is convenient for the use of various techniques and methods , analyze and observe the morphological and functional changes and internal mechanisms of cells under normal and changed living conditions; Many ethical problems are avoided. Therefore, in vitro cell culture has become one of the most commonly used experimental methods indispensable in various research fields of life ...

Claims

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Application Information

Patent Timeline
14 Mar 2007
Publication
CN1928063A
IPC
C12M3/00
Inventors
李宏; 刘佳