Screening method for colorectal cancer susceptibility genes

A susceptibility gene, colorectal cancer technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as abnormal proliferation and continued survival of cells, and achieve strong predictability, high detection rate, Highly Accurate Effects

Pending Publication Date: 2018-02-23
JIAXING YUNYING MEDICAL INSPECTION CO LTD
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Problems solved by technology

However, if the DCC gene cannot be expressed normally, resulting in the combination of the above-mentioned receptor

Method used

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  • Screening method for colorectal cancer susceptibility genes
  • Screening method for colorectal cancer susceptibility genes

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Embodiment Construction

[0045] A screening method for a colorectal cancer susceptibility gene of the present invention, comprising the following steps:

[0046] 1. Sample library preparation:

[0047] (1) Fragmentation by ultrasonic: the starting amount is 3 μg, add RNA-free-water to 100 μl and dilute to 30 ng / μl. The SCIENTZ08-Ⅲ cup-type ultrasonic cell crusher was used for ultrasonic fragmentation. The set parameters were: power 70%, interrupted for 3s, stopped for 1s, and circulated for 30-60min.

[0048] (2) Take out the magnetic beads half an hour in advance to return to room temperature, shake and mix, add 180ul magnetic beads to the PCR product after ultrasonic interruption, stir and mix, and incubate in the greenhouse for 5 minutes.

[0049] (3) Put the PCR single tube on the magnetic frame, let it stand for 5 minutes, remove the supernatant, keep the PCR tube on the magnetic frame, add 200 ul of 80% ethanol (newly prepared) for rinsing, and let it stand for 30 s and then remove the supernat...

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Abstract

The invention discloses a screening method for colorectal cancer susceptibility genes, which is quick and accurate and can cover all exon areas of newest colorectal cancer susceptibility genes. According to a basic scheme of the invention, the screening method for the colorectal cancer susceptibility genes comprises the following steps: extracting 3 to 5 ml of blood from an individual; extracting3 to 5 mu g of gDNA from the blood; breaking and amplifying the gDNA by utilizing ultrasound so as to construct a whole genome library of human, then, capturing breast cancer susceptibility genes by utilizing a colorectal cancer susceptibility gene scanning kit disclosed by the invention, and later on, performing high-throughput sequencing by utilizing a new generation sequencing instrument, and analyzing mutation information related to the genes to achieve the purpose of screening the colorectal cancer susceptibility genes.

Description

technical field [0001] The invention relates to a gene screening method, in particular to a colorectal cancer susceptibility gene screening method. technical background [0002] Colorectal cancer (colorectal cancer) is a malignant tumor that occurs in the large intestine. In western developed countries, its incidence ranks second in the spectrum of malignant tumors. In my country, there are 130,000 to 160,000 new cases of colorectal cancer every year, and the incidence rate is increasing at a rate of 4.2% every year, ranking third to fifth in cancer deaths. Colorectal cancer can be divided into adenocarcinoma, mucinous carcinoma and undifferentiated carcinoma according to histological type. Among them, adenocarcinoma accounts for three-quarters of colorectal cancer, and can be divided into three grades according to the degree of differentiation. Undifferentiated carcinoma has the lowest degree of differentiation and the highest degree of malignancy. The specific symptoms ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6869C12Q1/6886C12Q2600/156C12Q2535/122
Inventor 张道允巩子英叶建伟王伟
Owner JIAXING YUNYING MEDICAL INSPECTION CO LTD
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