The invention belongs to the technical field of tumor medicine, and particularly discloses an ELISA kit for screening of early cardia cancer. The kit comprises a solid phase carrier and a tumor-associated antigen coating the solid phase carrier, wherein the tumor-associated antigen is formed by EYA4, ABL1, CD38, P53, NOTCH1 and DLC1. Furthermore, the kit further comprises a sample diluent, a second antibody, a second antibody diluent, positive control serum, negative control serum, a developing solution, a stop solution and a washing solution. According to the ELISA kit disclosed by the invention, the cardia cancer, especially the early cardia cancer, can be effectively detected, the detection sensitivity is up to 92.2%, the specificity is up to 79.6%, the ELISA kit can be used for large-scale screening of an asymptomatic population in a high-incidence area of the cardia cancer, and is beneficial to screening and early discovery of the cardia cancer of the asymptomatic high-risk population.