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Kit for detecting susceptibility to lung cancer and SNP (Single Nucleotide Polymorphism) marker thereof

A technology of susceptibility and markers, applied in the field of genetic testing, can solve the problems that the genetic markers of common diseases cannot be widely used, lack of methods for early detection of common diseases, etc.

Inactive Publication Date: 2017-02-22
深圳市核子基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have discovered a large number of genetic markers associated with various common diseases. However, due to the lack of sufficient sensitivity and extensive (high-throughput detection sites, high-throughput detection samples) screening for multiple disease-related markers methods of investigation and testing, making the genetic markers of these common diseases not widely available
In addition, the current lack of systematic and effective integration of genetic markers for common diseases greatly restricts the development of early prevention, diagnosis and treatment of common diseases
Existing tests only detect genetic markers for a single disease or a group of diseases, and the detection range is limited, and there is no early detection method for some common diseases
[0005] At present, some traditional medical methods, such as tissue cell detection, have their inherent defects. Improper sampling location, insufficient tissue cell sample material or lack of experience will lead to misdiagnosis of nasopharyngeal carcinoma
Although other techniques such as imaging have been widely used in the examination and diagnosis of nasopharyngeal carcinoma, there are still great limitations in the qualitative determination of the degree of nasopharyngeal carcinoma

Method used

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  • Kit for detecting susceptibility to lung cancer and SNP (Single Nucleotide Polymorphism) marker thereof
  • Kit for detecting susceptibility to lung cancer and SNP (Single Nucleotide Polymorphism) marker thereof
  • Kit for detecting susceptibility to lung cancer and SNP (Single Nucleotide Polymorphism) marker thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 is used to detect SNP sites related to the susceptibility of lung cancer

[0054] Among them, rs3842 is located in the gene ABCB1 region, rs212090 is located in the gene ABCC1 region, rs1926203 is located in the gene ACTA2 region, rs8034191 is located in the gene AGPHD1 region, rs2808630 is located in the gene APCS region, rs2273535 is located in the gene AURKA region, rs3117582 is located in the gene BAG6 region, rs7216064 is located in the gene BPTF region, rs2131877 is located in the gene C3orf21 region, rs3817963 is located in the gene C6orf10 region, rs1801270 is located in the gene CDKN1A region, rs1051730 is located in the gene CHRNA3 region, rs402710 and rs31489 are located in the gene CLPTM1L region, rs9981861 is located in the gene DSCAM region, rs1656402 is located in the gene EIF4E2 region, rs1656402 is located in the gene EIF4E2 region, rs12 rs1047840 is located in the gene EXO1 region, rs11080466 is located in the gene FAM38B region, rs4254535 is ...

Embodiment 2

[0055] Example 2 Detection Kit for Lung Cancer Susceptibility

[0056] 1. Preparation of the kit:

[0057] 1. Design and synthesize PCR amplification primers and single-base extension primers for the 36 SNP sites. The PCR amplification primers and single base extension primers of the SNP sites to be tested are shown in Table 1

[0058] Table 1 PCR amplification primers and single base extension primers of SNP sites to be tested

[0059]

[0060]

[0061]

[0062] 2. The kit also includes Taq enzyme, dNTP mixture, MgCl 2 Solution, PCR reaction buffer, enzyme digestion reaction buffer, deionized water.

[0063] 2. The detection method of the kit:

[0064] 1. Extraction of DNA

[0065] 1.1 DNA extraction from oral swab

[0066] 1) Put the oral swab in a 2ml centrifuge tube and add 400μl PBS.

[0067] 2) Add 20 μl QIAGEN Protease and 400 μl Buffer AL. Immediately vortex for 15s to mix. To ensure efficient lysis, sample and Buffer AL must be mixed immediately and ...

Embodiment 3

[0116] The literature cited by 36 SNP sites closely related to lung cancer in Example 3 is shown in Table 5

[0117] Table 5 Literature cited by 36 SNP sites closely related to lung cancer

[0118]

[0119]

[0120]

[0121]

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for detecting the susceptibility to lung cancer. The SNP marker comprises 36 SNP loci. The invention further discloses a PCR (Polymerase Chain Reaction) amplification primer and a single-base extension primer of the SNP marker as well as a kit of the SNP marker. An important basis is provided for prevalence risk assessment and diagnostic reference of the lung cancer, so that early diagnosis of the lung cancer is realized.

Description

technical field [0001] The invention belongs to the field of genetic detection, in particular to a kit for detecting the susceptibility of lung cancer and its SNP marker. Background technique [0002] Lung cancer is currently the most malignant tumor in the world. Epidemiological studies have shown that more than 80% of lung cancers can be attributed to tobacco exposure, but less than 20% of smokers develop lung cancer, indicating that individuals with different genetic backgrounds have different susceptibility to lung cancer under the same environmental exposure. Single nucleotide polymorphisms play an important role. [0003] The association analysis method using single nucleotide polymorphism (Single Nucleotide Polymorphsm, SNP) as a genomic marker is currently one of the most commonly used methods for detecting genetic susceptibility genes of diseases. SNP refers to the DNA sequence polymorphism caused by single nucleotide variation at the genome level. The frequency o...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 张核子
Owner 深圳市核子基因科技有限公司
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