Kit for detecting gastric cancer susceptibility and SNP marker thereof

A technology of susceptibility and markers, applied in the field of genetic testing, can solve problems such as improper location of samples, lack of system integration, limitations, etc., and achieve the effects of high detection success rate, good technical reproducibility, and high practicability

CN106434979AInactive Publication Date: 2017-02-22深圳市核子基因科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2017-02-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an SNP marker for detecting gastric cancer susceptibility. The SNP marker comprises 28 SNP loci. The invention further discloses a PCR (polymerase chain reaction) amplification primer, a single base extension primer and a kit of the SNP marker. Literature demonstrates that the 28 SNP loci have high practicability, can be used for early evaluation and large-scale screening of gastric cancer and are high in detection survival rate, technological repeatability and performance-cost ratio, and an important basis is provided for disease risk evaluation and diagnosis reference of the gastric cancer.
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Description

technical field

[0001] The invention belongs to the field of genetic detection, in particular to a kit for detecting gastric cancer susceptibility and its SNP marker. Background technique

[0002] Gastric cancer is a disease that seriously endangers human health, ranking second in the incidence of tumors and cancer mortality in the world. The occurrence of gastric cancer is mainly related to environment, Helicobacter pylori and genetic factors. In recent years, the single nucleotide polymorphisms of some genes have been paid attention to in the study of gastric cancer susceptibility.

[0003] The association analysis method using single nucleotide polymorphism (Single Nucleotide Polymorphsm, SNP) as a genomic marker is currently one of the most commonly used methods for detecting genetic susceptibility genes of diseases. SNP refers to the DNA sequence polymorphism caused by single nucleotide variation at the genome level. The frequency of occurrence in the population is gr...

Examples

Embodiment 1

[0045] Example 1 Used to detect SNP sites related to gastric cancer susceptibility

[0046] Among them, rs16861205 is located in the gene ADIPOQ region, rs10773989 and rs1044471 are located in the gene ADIPOR2 region, rs321298 is located in the gene CD3EAP region, rs187116 is located in the gene CD44 region, rs121964871, rs26160 and rs17690554 are located in the gene CDH1 region, rs1880481 is located in the gene EGNNB7 region 45 region, rs2 rs1799793 is located in the region of gene ERCC2, rs11466306 is located in the region of gene FAM136A, rs889312 is located in the region of gene MAPK3K1, rs2279744 is located in the region of gene MDM2, rs3732183 and rs2303428 are located in the region of gene MSH2, rs1801133 is located in the region of gene MTHFR, rs4072037 is located in the region of gene MUC16, rs4648 is located in the region of gene KBNF0 rs2274223 is located in the gene PLCE1 region, rs2076167 and rs3734254 are located in the gene PPARD region, rs13361707 is located in ...

Embodiment 2

[0047] Example 2 Kit for detecting susceptibility to gastric cancer

[0048] 1. Preparation of the kit:

[0049] 1. Design and synthesize PCR amplification primers and single-base extension primers for the 28 SNP sites. The PCR amplification primers and single base extension primers of the SNP sites to be tested are shown in Table 1

[0050] Table 1 PCR amplification primers and single base extension primers of SNP sites to be tested

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[0054] 2. The kit also includes Taq enzyme, dNTP mixture, MgCl 2 Solution, PCR reaction buffer, enzyme digestion reaction buffer, deionized water.

[0055] 2. The detection method of the kit:

[0056] 1. Extraction of DNA

[0057] 1.1 DNA extraction from oral swab

[0058] 1) Put the oral swab in a 2ml centrifuge tube and add 400μl PBS.

[0059] 2) Add 20 μl QIAGEN Protease and 400 μl Buffer AL. Immediately vortex for 15s to mix. To ensure efficient lysis, sample and Buffer AL must be mixed immediatel...

Embodiment 3

[0106] Example 3 The literature cited by 28 SNP sites closely related to gastric cancer, see Table 5

[0107] Table 5 Literature cited by 28 SNP sites closely related to gastric cancer

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