216 results about "Mass screening" patented technology

RBC and platelet (Plt) alloimmunization requires antigen-matched blood to avoid adverse transfusion reactions. Some blood collection facilities use unregulated Abs to reduce the cost of mass screening, and later confirm the phenotype with government approved reagents. Alternatively, RBC and Plt antigens can be screened by virtue of their associated single nucleotide polymorphisms (SNPs). We developed a multiplex PCR-oligonucleotide extension assay using the GenomeLab SNPStream platform to genotype blood for a plurality of blood group antigen-associated SNPs, including but not limited to: RhD (2), RhC / c, RhE / e, S / s, K / k, Kpa / b, Fya / b, FYO, Jka / b, Dia / b, and HPA-1a / b.

The invention discloses a high-efficiency sand screening machine for a building construction site, which comprises a bottom plate, a support frame connected to both sides of the top of the bottom plate, a top plate connected to the top of the support frame, a driving gear connected to the middle of the top plate, and a guide rod fixed in the middle of the top plate. There is a guide sleeve in the middle of the guide rod, the lower end of the guide sleeve is connected with the left rack, the left lower end of the left rack is connected with the left moving rod, the lower end of the top plate is fixed with a slide rail, the middle of the slide rail is provided with a slider, and the upper end of the slider is connected with a right Rack, the right moving rod is connected to the lower side of the right end of the right rack, the upper end of the screen is provided with a crushing shaft, the right end of the crushing shaft is connected to a crushing pulley, the upper end of the crushing pulley is connected to a driven pulley through a belt, and the left end of the driven pulley A pulley shaft is connected, the left end of the pulley shaft is connected with a driving gear, and the front end of the driving gear is meshed with a driven gear. The present invention realizes the requirement of crushing the coarse sand remaining on the upper end of the screen, increases the screening quality of the screen, and avoids the later crushing treatment of the coarse sand.

The invention provides two mouse bone marrow hybridoma cell strains, a monoclonal antibody generated by the same and application, canine distemper virus resistant monoclonal antibodies 6E11 and 1G5 respectively secrete by the mouse bone marrow hybridoma cell strains, a prepared detecting kit and pharmaceutical composition containing the monoclonal antibodies 6E11 and 1G5, and application of the detecting kit and pharmaceutical composition, and belongs to the technical field of biology. The prepared canine distemper virus antigen detection kit containing the monoclonal antibodies 6E11 and 1G5 is high in sensitivity, and is easy and convenient to operate, quick and time-saving, or the detection rate of CDV infection is improved, application in the aspect of large-scale screening is facilitated, and the prepared canine distemper virus antigen detection kit containing the monoclonal antibodies 6E11 and 1G5 is suitable for quick detection in grassroots or in site.

The invention discloses a kit for one-time qualitative screening of common fourteen mutational sites of deaf gene of Chinese populations and a use method thereof. The kit takes total fourteen sites of the deaf gene of the Chinese populations as detection objects. Application primers and extension primers are respectively designed for the mutation of each site, multiple PCR amplification and markup extension are simultaneously carried out on each target zone, and genotypes of above fourteen sites can be obtained at once through capillary electrophoretic analysis. The screening method and the kit of the invention have advantages of convenient use, simple operation, low cost, high flux, and direct and reliable detection result, and are suitable for large scale screening of the deaf gene mutation of the Chinese populations.

The invention belongs to the technical field of biology, and particularly relates to an ovarian tumor serum marker. The serum of a patient is subjected to multi-level analysis with high flux and large scale by adopting color spectrum and mass spectrum technology so as to screen serum differential expression molecules, and the serum of the ovarian tumor patient is subjected to metabonomics and polypeptide spectrum analysis of different levels to determine a small molecular metabolism spectrum so as to find a plurality of groups of characteristic biomarkers related with benignant, borderline and malignant ovarian tumor. The invention is possible to provide a simple method for screening the ovarian tumor of different stages in large scale, and also can provide valuable reference for differential diagnosis of related clinical patients.

A new method and apparatus for detecting outliers, more specifically false-negatives and / or false-positives, in pharmaceutical mass screening experiments is provided which utilizes chemical descriptor methodology in conjunction with supervised learning techniques. This method employs the latent structure-activity relationship between the chemical compounds and the biological activity for the detection of such outliers. The method is applicable to individual compounds as well as to pools or mixture of compounds.

The present invention provides a method for measuring diacylglycerol acetyltransferase (DGAT) activity which utilizes a novel solvent system to reduce and / or eliminate the activities of related compounds. The present invention also discloses a method for determining whether a compound is useful for modulating DGAT biological activity. The method is capable of being utilized for mass screening of compounds as modulators of the biological activity of DGAT.

The invention belongs to the technical field of tumor medicine, and particularly discloses an autoantibody joint detection ELISA kit for screening early esophageal cancer. The kit comprises a solid-phase carrier and tumor-associated antigens coated on the solid-phase carrier, wherein the tumor-associated antigens are EYA4, ABL1, ATF2, CD38, NOTCH1, ZBTB20, BCL2L1 and P53. Furthermore, the kit alsocomprises a sample diluent, a second antibody, a second antibody diluent, positive control serum, negative control serum, a color developing solution, a stop solution and a washing solution. The ELISA kit provided by the invention can effectively detect esophageal cancer, especially early esophageal cancer, has the detection sensitivity of 94% and the specificity of 79%, can be used for large-scale screening of asymptomatic people in high-incidence areas of esophageal cancer, and is beneficial to screening and early discovery of asymptomatic high-risk population.

The invention belongs to a method for extensively screening scallop SNP in the technical field of the scallop DNA molecular genetic marker, which comprises the following steps that: solution D and phenol-chloroform are firstly used for extracting a plurality of Chlamys farreri RNA, and a ultraviolet specrophotometer is used for measuring the concentration of the mixture of solution D and the phenol-chloroform which are uniformly mixed; a scallop full-length cDNA sample is re-established and comprises the synthesis of a cDNA first chain and the synthesis and augmentation of a cDNA second chain; then homogenization of full-length cDNA is performed, and ultrasonic breaking is performed; then sequence measuring joints are connected, and terminal repair, joint connection and sample augmentation are included; finally a biological software is used for analyze the data to obtain an SNP marker; then an SNP marker to be screened is selected to design a primer; and DNA of individual scallop is extracted to be equivalently mixed to be used as a template, conventional colony sequence measuring is undertaken after the PCR augmentation, and the position point with the occurring frequency of single base difference being 10 percent or more is defined as an SNP marker. The method has safe and reliable principle, simple and controllable flow procedures, reliable running of large-scale screening, excellent effect and strong practicability.

The invention provides efficient methods for the isolation of centromeres from potentially any organism. Using the methods of the invention, methylated centromere DNA may be isolated from potentially any centromere in an organism. The technique is amenable to mass screenings employing use of arrays comprising libraries of DNA from a target species.

The invention relates to a microRNA marker for detecting acute mountain sickness, and application thereof to preparation of a kit for detecting acute mountain sickness. The content of microRNA-676-3p, microRNA-181b-5p, microRNA-193b-5p and microRNA-3591-3p in the blood of a testee is detected by means of the quantitative PCR method and the like, and is compared with the normal microRNA level to diagnose acute mountain sickness. The acute mountain sickness molecular marker microRNA has high sensitivity and specificity, operation is convenient, safe and nondestructive, mass screening can be achieved easily, and the effect of combined use is better.

The invention discloses a power supply test system, which comprises a high-low temperature test box, a program-controlled power supply, a program-controlled load, a channel control card and a data acquisition device, wherein the high-low temperature test box, the program-controlled power supply, the program-controlled load, the channel control card and the data acquisition device are connected with test control equipment via communication interfaces. The invention also discloses a power supply test method. According to the system and the method, mass screening test can be carried out on communication power supplies in a simulated limit working environment, the quality and reliability of communication power supplies leaving the factory can be improved, and a single program-controlled power supply and a single program-controlled load can be used for carrying out screening test on a plurality of communication power supplies.

The invention discloses a breast ultrasonic screening method which comprises the following steps: acquiring a depth image of a chest area of a user; performing model reconstruction according to the depth image so as to obtain a three-dimensional structure model of an area to be scanned, and generating a scanning track of an ultrasonic probe according to the three-dimensional structure model; generating a motion control code according to the scanning track, and inputting the motion control code into a scanning mechanism to control the scanning mechanism to drive the ultrasonic probe to conductultrasonic scanning on the breast area of the user; analyzing and processing the obtained ultrasonic image, and generating diagnosis results. With the help of automation technology and artificial intelligence technology, the breast ultrasonic screening method disclosed by the invention can make mass screening of breast cancer with low cost in a large scale possible, greatly increases the proportion of Chinese women of right ages to participate in breast cancer screening and contributes to prevention and control of breast cancer.

A preparation method for a urine monohydroxyphenyl metabolites detection reagent comprises the steps of preparing solution A, preparing solution B, preparing solution C, uniformly mixing solution A, solution B and solution C at the volume ratio of 1:(1.5-2):(0.5-1.5), adding distilled water into the mixture, and stirring the mixture uniformly, wherein solution A is a mixture prepared through reacting 1 part by weight of metallic mercury with 2-2.2 parts by weight of nitric acid and being diluted by distilled water; solution B is a nickel nitrate solution with the concentration of 0.05g / ml-0.15g / ml; solution C is a mercuric sulfate solution with the concentration of 0.05g / ml-0.15g / ml. The process for preparing testing reagents of monohydroxyphenyl metabolites in urine has the advantages of being low in production cost, and the prepared testing reagents of monohydroxyphenyl metabolites in urine is high in stability and sensitivity, low in false positive rate and false negative rate, and suitable for mass screening and clinical examination.

The invention belongs to the technical field of medical oncology, and particularly discloses an autoantibody joint detection ELISA kit for early screening of esophageal squamous carcinoma. The kit comprises a solid-phase carrier and a tumor-associated antigen coated on the solid-phase carrier, wherein the tumor-associated antigen consists of P53, TP53, CDKN2B and NPM1. Furthermore, the kit comprises sample diluent, a second antibody, second antibody diluent, positive control serum, negative control serum, developing solution, stop solution and washing solution. The ELISA kit is capable of effectively detecting the esophageal squamous carcinoma, especially the early esophageal squamous carcinoma, has detection sensitivity up to 88.8% and specificity up to 86.3%, can be used for the large-scale screening of asymptomatic groups in high-risk areas of the esophageal squamous carcinoma, and is beneficial for the esophageal squamous carcinoma screening and early discovery of the asymptomatichigh-risk groups.

The invention provides an avian leukosis double-antibody sandwich enzyme-linked immuno sorbent assay (ELISA) antigen detection kit. The kit comprises ELISA plates which are coated on anti-avian leukosis virus (ALV) p27 monoclonal antibodies, enzyme labeling anti-ALVp27 monoclonal antibodies and the like. Antibody capturing and antibody detecting are respectively conducted aiming at different antigenic determinants of p27 protein; anti-ALVp27 monoclonal antibodies coated by the ELISA plates are obtained through secretion of hybridoma cell strain ALVP27-5D3, and the enzyme labeling anti-ALVp27 monoclonal antibodies are obtained through secretion of hybridoma cell strain ALVP27-4F12. The avian leukosis double-antibody sandwich ELISA antigen detection kit is easy, convenient and fast to operate, can be used in detecting of all subgroup virus of ALV, and is suitable for all levels of veterinarian departments in the basic level and rapid and mass screening detecting of the avian leukosis of leaving and entering the country. The avian leukosis double-antibody sandwich ELISA antigen detection kit has the advantages of being low in cost, notable in economical benefit, and wide in application prospect.

The invention relates to a kit for SNP typing of obesity-prone genes of Chinese population and a using method of the kit, and particularly discloses a simultaneous qualitative typing kit aiming at twelve SNP sites of ten obesity-prone genes of the Chinese population and a using method of the simultaneous qualitative typing kit. Two sites of an FTO gene, one site of an MC4R gene, one site of an ADRB3 gene, one site of a PPAR gamma 2 gene, one site of an LEPR gene, two sites of an APOE gene, one site of a UCP 2 gene, one site of a GNPDA2 gene, one site of a KCTD15 gene and one site of an NEGR 1 gene are taken as detection objects, an amplification primer and an extension primer are designed for mutation of each SNP site, multiple PCR amplification and markup extension are performed, and genotypes of the twelve SNP sites are acquired simultaneously through capillary electrophoresis analysis. The kit is convenient to use, simple to operate, low in cost, high in flux, direct and reliable in detection result and suitable for large-scale screening of SNP typing of the obesity-prone genes of the Chinese population.

The invention discloses a rapid sensitive method for detection of the freshness of meat. The method uses an ion mobility spectrometry detection technology as the basic technology, and employs positive and / or negative ion mode for the establishment of an ion mobility spectrometry method for the rapid detection of the freshness of meat. Under the experimental conditions, the changes of released components and content variation of the same component in the spoilage process of meat can be monitored continuously. A detection device provided by the invention has the advanategs of high sensitivity, easy assembly, low cost, easy popularization, and suitability for on-site rapid detection and mass screening. The method can rapidly and sensitively detect freshness of meat and increase the efficiency and accuracy of food inspection and quarantine.

The invention belongs to the technical field of biology and particularly relates to an application of hsa_circ_0049154 as a prostatic cancer molecular target in preparation of a drug and a kit. The nucleotide sequence of hsa_circ_0049154 is shown in SEQ ID NO.1. The application comprises an application of the prostatic cancer molecular target in screening a drug for preventing, relieving and / or treating prostatic cancer, an inhibitor of the prostatic cancer molecular target and an application of the inhibitor in preparing a drug for preventing, relieving and / or treating prostatic cancer, a prostatic cancer diagnosis kit containing the prostatic cancer molecular target as well as an application of the prostatic cancer molecular target and the diagnosis kit in screening, diagnosis, treatment, situation monitoring and prognosis monitoring of high-risk groups of prostatic cancer. When used for diagnosing the prostatic cancer, the molecular target and the diagnosis kit containing the molecular target have the characteristics of being simple to operate, convenient in material taking, safe, free from wound, higher in specificity and sensitivity and capable of facilitating mass screening.

The invention provides a high-throughput low-cost SNP (single nucleotide polymorphism) genotyping method based on a liquid molecular hybridization principle. The method comprises the following steps: extracting biological genome DNA (deoxyribonucleic acid) and carrying out biotin labeling on the biological genome DNA; designing site-specific hybridization primers LSP1 and LSP2 and carrying out 5' phosphorylation on LSP2; hybridizing an LSP1 mixture and an LSP2 mixture with the genome DNA to obtain a hybridization adsorption product; carrying out extended linkage reaction to obtain a target DNA fragment; carrying out a round of PCR (polymerase chain reaction) amplification on a universal primer; carrying out PCR amplification on the Barcode specific primer of the recovered target fragment; carrying out high-throughput sequencing; obtaining SNP site genotyping information through analysis. The method combines the site selection flexibility of the liquid hybridization technology with the advantages of high throughput and low cost of the high-throughput sequencing technology and has great application value and wide popularization prospect in the research fields such as large-scale screening of SNP, genome-wide association study, population diversity evaluation, gene function analysis and the like.

The invention discloses a mammary-cancer-susceptible-gene detection chip which comprises a solid support and a gene detection probe fixed onto the solid support. The gene detection probe comprises 31 SNP (single-nucleotide polymorphism) loci of 24 mammary-cancer-susceptible genes. The 24 mammary-cancer-susceptible genes are respectively CHEK2, PALB2, ZNF350, ESR1, C6orf97, USP7, MRPS30, AURKA, ATM, CASC16, TOX3, DNMT1, ZMIZ1, TGFB1, FGFR2, ZNF365, SLC4A7, TNRC9, TNP1, HER2, BRCA2, CCDC170, BRCA1 and CYP11A1. The gene detection probe comprises the 31 SNP loci of the 24 mammary-cancer-susceptible genes. The documents prove that the loci have higher practicality, can be used for large-scale screening of early diagnosis of diseases, have the advantages of high detection success rate, favorable technique reproducibility and high cost performance, and provide important references for diagnosis, typing and prognosis of mammary cancer, thereby implementing early diagnosis of diseases.

The invention discloses a quick detection method of meat freshness and a detection platform thereof, which belong to the electrochemical field. The invention is based on an electrochemical luminescence theory, has very good response to inorganic ammonia with an MWNTs / PVA / (pq)2Ir(N-phMA) modified electrode, takes an MWNTs / PVA / iridium compound modified electrode as a working electrode, a platinum wire electrode as a counter electrode and an Ag / AgCl electrode as a reference electrode, carries out CV-ECL scanning with the scanning scope of 0.2 to 1.8V and the scanning rate of 100mv / s, can continuously monitor the ammonia produced in the meat corruption change process, can quickly and sensitively detect the meat freshness, and improves the food quarantine efficiency and accuracy. The detection platform provided by the invention has the advantages of easy assembly, low cost and easy popularization, and is applicable to on-site rapid detection and mass screening.

Lactobacillus screening methods were carried out using surface plasmon resonance spectrums and human intestinal mucin and blood group antigens as probes. A trial to set selection criteria in the above-mentioned methods of screening for lactobacilli was made to adapt the methods to mass screening, and it was discovered that lactobacilli compatible with ABO blood groups can be screened by setting 100 RU as a criterion for judging bacterial binding under certain conditions. Using 238 lactobacillus strains, the above-mentioned screening methods and tests to judge their compatibility for the use of yogurt production were carried out, to at long last specifically discover bacillus strains compatible with blood groups A, B, and O.

The invention discloses a soybean screening machine based on mass separation, which comprises a base, a screening box, a vibrating motor, a first screening net, a second screening net, a third screening net, a dust removal box, a filter cloth and an exhaust fan , the upper end of the shock-absorbing spring is fixedly connected to the screening box, and vibration motors are installed on the outside of the front end and the outside of the rear end of the screening box, and the inside of the first chute is embedded with a first screening net, a second screening net and the third screening net, a dust removal box is installed on the left side of the upper end of the base, a filter cloth is embedded inside the second chute, and an exhaust fan is installed at the bottom right inside the dust removal box. The structure of the present invention is scientific and reasonable, and can be used It is safe and convenient, equipped with components such as a dust removal box, which can quickly suck the internal dust into the dust removal box when the soybean screening machine is screening, and filter the smoke dust. The operator can breathe fresh air and the environment is protected.

The invention discloses a gastric cancer detection serum / plasma miRNA (micro ribonucleic acid) marker and an application thereof. The biomarker is any one or more out of miR-148a, miR-142, miR-26a and miR-195, wherein miR-148a is as shown in SEQIDNO:1; miR-142 is as shown in SEQIDNO:2; miR-26a is as shown in SEQIDNO:3; miR-195 is as shown in SEQIDNO:4. According to the marker and the application thereof, a gastric cancer patient tissue miRNA chip expression profile is combined with a serum / plasma miRNA chip expression profile for use for the first time, and the screened miRNA marker is tumor-specific, high in sensitivity and applicable to large-scale screening, and gastric cancer can be simply diagnosed.

The invention belongs to the technical field of the tumor medicine, and specifically discloses an autoantibody joint detection ELISA kit for gastric cardiac adenocarcinoma early screening. The kit comprises a solid-phase carrier and tumor-associated antigen coated on the solid-phase carrier; the tumor-associated antigen is composed of Dock10, IVL, CDH1 and P53; furthermore, the kit further comprises sample diluent, a second antibody, second antibody diluent, positive control serum, negative control serum, a developing solution, a stop solution and a washing solution. The ELISA kit disclosed bythe invention can effectively detect the gastric cardiac adenocarcinoma, especially the early gastric cardiac adenocarcinoma; the detection sensitivity is up to 87.5%, and the specificity reaches 75%; and the kit can be used for the large-scale screening of the asymptomatic population at the high incidence area of the gastric cardiac adenocarcinoma, thereby facilitating the gastric cardiac adenocarcinoma screening and early discovery of the asymptomatic high-risk group.

The invention belongs to the field of biotechnology and neurocognitive and relates to a serum / plasma micro-RNA marker for detecting patients with mild cognitive impairment and an application thereof. The maker is any one or a combination of miR-206 and miRNA-132. The marker is applied in diagnosis of patients with mild cognitive impairment, and the marker is applied in preparation of a kit for diagnosis of patients with mild cognitive impairment. According to the application of the marker, content of serum / plasma miR-206 and / or miRNA-132 of a testee is detected by a real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) method, and the content of the testee is compared with that of a normal cognitive subject to diagnose mild cognitive impairment. The screened miRNA marker has cognitive impairment specificity, is simple to operate diagnosis of mild cognitive impairment, has high sensitivity and is suitable for large-scale screening.

The invention belongs to the biotechnology field and in particular provides a prostatic cancer molecular target RP11-1023L17.1 and an application thereof to a diagnostic kit. RP11-1023L17.1 is IncRNA (long non-coding ribonucleic acid). A nucleotide sequence of RP11-1023L17.1 is shown in SEQ ID NO.1. The invention comprises an application of the prostatic cancer molecular target to screening drugs for preventing, relieving and / or treating prostatic cancers, an inhibitor of the prostatic cancer molecular target, an application of the inhibitor to preparation of drugs for preventing, relieving and / or treating prostatic cancers, the prostatic cancer diagnostic kit containing the prostatic cancer molecular target and applications of the prostatic cancer molecular target and the diagnostic kit to screening, diagnosis, treatment, status monitoring and prognostic monitoring of prostatic cancer high risk groups. Being used for diagnosing prostatic cancers, the molecular target and the diagnostic kit containing the molecular target are simple to operate and convenient to draw, are safe and non-invasive and have the characteristics of higher specificity and sensitivity and easiness in mass screening.

The invention belongs to the field of biological medicine and specifically relates to various RNAs, various combinations of tumor markers thereof and an application thereof in preparing a gastric cancer diagnosis reagent. The invention provides a set of new RNA molecular markers for gastric cancer diagnosis. The content of the set of RNA in gastric cancer tissue is higher than the content of para-carcinoma tissue and the content of the set of RNA in the plasma of gastric cancer patient is higher than the content thereof in the plasma of healthy people. The invention also provides a method for taking one or different combinations of the set of RNA as the tumor markers and the application thereof. The set of RNA molecular marker and the diagnostic method of different combinations of the set of RNA markers adopted for diagnosing gastric cancer are simple in operation, are convenient in material taking and have the characteristics of high specificity, high sensitivity and easiness in mass screening. The set of RNA molecular marker is fit for screening of gastric cancer high-risk groups and auxiliary diagnosis of gastric cancer.