Liquid biopsy ELISA kit for early screening of esophageal cancer high-risk groups

Abstract

The invention belongs to the technical field of bioengineering and biomedicine, particularly belongs to the field of molecular biology and oncology, and more particularly relates to a liquid biopsy ELISA kit for early screening of esophageal cancer high-risk groups. The kit comprises a solid-phase carrier and tumor-associated antigens TVP23C, PAQR5 and NUBPL coated on the solid-phase carrier, andfurther comprises a sample diluent, a second antibody, a second antibody diluent, negative control serum, positive control serum, a washing solution, a developing solution and a stop solution. The kithas esophageal cancer detection sensitivity of 91.46% and esophageal cancer detection specificity of 83.75%, and is suitable for large-scale screening of asymptomatic people in esophageal cancer high-incidence areas.

Description

technical field [0001] The invention belongs to the technical fields of bioengineering and biomedicine, relates to the fields of molecular biology and oncology, and more specifically relates to a group of liquid biopsy ELISA kits for early screening of high-risk groups of esophageal cancer. Background technique [0002] Esophageal cancer is a common digestive tract tumor with a high incidence rate, second only to gastric cancer. my country is one of the areas with a high incidence of esophageal cancer in the world, with an average of 150,000 deaths per year. Esophageal cancer is the most common tumor in rural my country. Esophageal cancer is a malignant tumor that occurs in the epithelial tissue of the esophagus. The occurrence of esophageal cancer is related to the chronic stimulation of nitrosamine compounds, inflammation and trauma, genetic factors, and the content of trace elements in drinking water, food and vegetables. Generally speaking, the pathogenic factors of es...

AI Technical Summary

Problems solved by technology

It has been confirmed that the surgical resection rate of early detection of esophageal cancer can reach 100%, and the 5-year survival rate can reach more than 90%.

Moreover, the application of multiple combined detection methods and corresponding kits for the early diagnosis of esophageal cancer is relatively rare.

Early detection of asymptomatic high-risk groups of esophageal cancer is particularly important. However, during gastroscopy and other screening, the subjects need to endure certain pains, are not easy to obey, and the detection machines are expensive. Gastroscopy cannot be popularized in remote areas.

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