Liquid biopsy ELISA kit for early screening of esophageal cancer high-risk groups

A liquid biopsy, kit technology, applied in biological testing, material testing products, material analysis by observing the impact on chemical indicators, etc., can solve the problem of unsatisfactory long-term efficacy, inability to popularize gastroscopy, and expensive testing machines. and other problems, to achieve the effect of improving detection efficiency and diagnosis efficiency, facilitating screening and early detection, and high detection success rate

Active Publication Date: 2020-08-18
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been confirmed that the surgical resection rate of early detection of esophageal cancer can reach 100%, and the 5-year survival rate can reach more than 90%.
Moreover, the application of multiple combined detection methods and corresponding kits for the early diagnosis of esophageal cancer is relatively rare.
Early detection of asymptomatic high-risk groups of esophageal cancer is particularly important. However, during gastroscopy and other screening, the subjects need to endure certain pains, are not easy to obey, and the detection machines are expensive. Gastroscopy cannot be popularized in remote areas.

Method used

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  • Liquid biopsy ELISA kit for early screening of esophageal cancer high-risk groups
  • Liquid biopsy ELISA kit for early screening of esophageal cancer high-risk groups
  • Liquid biopsy ELISA kit for early screening of esophageal cancer high-risk groups

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Liquid biopsy ELISA kit for early screening of high-risk groups of esophageal cancer

[0028] 1. Composition of the kit

[0029] The composition of kit of the present invention is as follows:

[0030](1) A microtiter plate coated with three antigens: TVP23C, PAQR5, and NUBPL. The layout of the three antigens on the microtiter plate is as follows: figure 1 shown;

[0031] (2) Sample diluent: PBST (phosphate Tween) buffer containing 1% (W / V) BSA;

[0032] (3) Secondary antibody diluent: PBST (phosphate Tween) buffer containing 1% (W / V) BSA;

[0033] (4) Enzyme-labeled secondary antibody: horseradish peroxidase-labeled RecA protein (purchased from Invitrogen);

[0034] (5) Color-developing solution: The color-developing solution is composed of color-developing solution A and color-developing solution B, wherein, color-developing solution A is 0.02% (W / V) TMB (3,3',5,5'-tetramethyl base benzidine), the color developing solution B is 0.006% (W / V) urea peroxide...

Embodiment 2

[0064] Example 2 Analysis of the effectiveness of the kit of the present invention in the detection of esophageal cancer

[0065] The kit described in Example 1 of the present invention was used to detect the serum samples of patients with early esophageal cancer and normal people, so as to evaluate and analyze the value of the kit of the present invention for screening and diagnosis of early esophageal cancer.

[0066] sample source

[0067] Serum samples were collected from the Henan Province Esophageal Cancer Key Open Laboratory of the First Affiliated Hospital of Zhengzhou University, including 480 samples of normal human serum (control group) and 480 serum samples of patients with early-stage esophageal cancer (early-stage esophageal cancer group). 480 cases of normal human serum were collected from the healthy physical examination center of the laboratory's cooperative hospital, without any tumor-related evidence. Among the 480 normal subjects, there were 228 males and ...

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Abstract

The invention belongs to the technical field of bioengineering and biomedicine, particularly belongs to the field of molecular biology and oncology, and more particularly relates to a liquid biopsy ELISA kit for early screening of esophageal cancer high-risk groups. The kit comprises a solid-phase carrier and tumor-associated antigens TVP23C, PAQR5 and NUBPL coated on the solid-phase carrier, andfurther comprises a sample diluent, a second antibody, a second antibody diluent, negative control serum, positive control serum, a washing solution, a developing solution and a stop solution. The kithas esophageal cancer detection sensitivity of 91.46% and esophageal cancer detection specificity of 83.75%, and is suitable for large-scale screening of asymptomatic people in esophageal cancer high-incidence areas.

Description

technical field [0001] The invention belongs to the technical fields of bioengineering and biomedicine, relates to the fields of molecular biology and oncology, and more specifically relates to a group of liquid biopsy ELISA kits for early screening of high-risk groups of esophageal cancer. Background technique [0002] Esophageal cancer is a common digestive tract tumor with a high incidence rate, second only to gastric cancer. my country is one of the areas with a high incidence of esophageal cancer in the world, with an average of 150,000 deaths per year. Esophageal cancer is the most common tumor in rural my country. Esophageal cancer is a malignant tumor that occurs in the epithelial tissue of the esophagus. The occurrence of esophageal cancer is related to the chronic stimulation of nitrosamine compounds, inflammation and trauma, genetic factors, and the content of trace elements in drinking water, food and vegetables. Generally speaking, the pathogenic factors of es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/68
CPCG01N21/78G01N33/6854
Inventor 王立东杨媛啧赵学科宋昕王苒李留玉李贝范宗民韩雪娜雷玲玲常志伟
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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