A SNP marker and kit for screening high-risk groups of cardiac cancer

A cardia cancer and kit technology, applied in the fields of genetic engineering and oncology, can solve the problems of SNP sites and kits for high-risk groups of cardiac cancer, and achieve early screening convenience, ease of detection, high sensitivity and specific effect

Active Publication Date: 2020-04-10
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports of SNP sites and kits for screening high-risk populations of cardiac cancer

Method used

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  • A SNP marker and kit for screening high-risk groups of cardiac cancer
  • A SNP marker and kit for screening high-risk groups of cardiac cancer
  • A SNP marker and kit for screening high-risk groups of cardiac cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Screening for detecting SNP sites related to susceptibility to cardia cancer

[0063] The inventors conducted genome-wide association analysis on peripheral blood DNA of 1528 patients with cardiac cancer and 6936 healthy people collected from areas with high incidence of cardiac cancer in my country (Linzhou City, Anyang City, Huixian City, Henan Province, etc.). There were 15 SNP loci that were significantly related to cancer. Among them, 1528 patients with cardiac cancer were pathologically diagnosed cardiac cancer patients, and 6936 healthy people were pathologically confirmed without cardiac cancer.

[0064] The specific operation of screening the 15 SNP loci significantly related to the incidence of cardia cancer is as follows:

[0065] 1. DNA extraction from peripheral blood: Extract DNA from the sample according to the instructions of the QIAGEN genomic DNA extraction kit. The specific steps are:

[0066] (1) Cell thawing: Take the blood sample out of ...

Embodiment 2

[0081] Example 2: Detection of susceptibility gene loci and screening of high-risk groups in asymptomatic people in 10,000 cases of cardiac cancer high-incidence areas

[0082] Select 10,000 asymptomatic people aged 50-60 in high-incidence areas of cardia cancer (Linzhou City, Anyang City, Huixian City, Changzhi City, etc.), and extract their peripheral blood DNA. The genotypes of the 15 SNP loci that were significantly associated with the above 10,000 asymptomatic patients were divided into high-risk and non-high-risk groups, and the two groups were followed up for 8 years and confirmed by gastroscopy and biopsy. The prevalence of cardia cancer in the two groups was analyzed, and statistical analysis was performed to determine the sensitivity and specificity of 15 SNP loci in screening high-risk populations for cardia cancer.

[0083] The specific operation steps are as follows:

[0084] 1. DNA extraction from peripheral blood: Extract the sample DNA according to the instruc...

Embodiment 3

[0131] Example 3: Production of a kit for screening high-risk and high-risk populations of cardia cancer

[0132] The production and operation process of the screening kit for high-risk and high-risk groups of cardia cancer is based on Affymetrix 6.0 chip detection and Sequenom MassARRAY genotyping technology. Specific amplification primers and specific extension primers for SNP sites that are significantly associated , dNTP mixture, MgCl 2 Solutions, PCR reaction buffers, enzyme digestion reaction buffers, deionized water, etc., these commonly used reagents are well known to those skilled in the art; in addition, there may also be standards and controls (such as standards and blanks for determining genotypes) control, etc.). The detection method of the kit includes: (1) DNA extraction from peripheral blood; (2) PCR amplification; (3) alkaline phosphatase treatment of PCR products; (4) single base extension; (5) resin purification; (6) ) chip spotting; (7) steps such as mas...

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Abstract

The invention belongs to the field of genetic engineering and tumor medicine, and particularly discloses an SNP marker and kit for screening out and diagnosing a gastric cancer high-risk group. The SNP marker is a combination of rs2274223, rs13042395 rs7447927, rs1642764 rs35597309, rs10074991, rs2294693, rs4072037, rs10931936, rs13016963, rs10201587, rs7578456, rs9288318, rs1801133 and rs1048943.The invention further discloses a kit for screening out and the gastric cancer high-risk group. The SNP marker combination can be used for early evaluating and large-scale screening of the gastric cancer, is high in detection success rate and technical reproducibility, defines the gastric cancer high-risk group to the maximum extent, makes the early gastric cancer detection rate reach 71.4%, andprovides an important basis for screening out the gastric cancer high-risk group and evaluating the disease risk.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and tumor medicine, and in particular relates to a SNP marker and a kit for screening and diagnosis of high-risk groups of cardia cancer. Background technique [0002] The cardia is located at the esophagogastric junction within 2cm below the dentate line. Due to the small anatomical range of the cardia, the tissue invasion of this site after canceration is wider, and it is difficult to accurately locate the tumor site. Therefore, internationally, tumors occurring in the lower esophagus and gastric junction are collectively referred to as esophagogastric junction adenocarcinoma, and are classified as esophagogastric junction adenocarcinoma. It is divided into three categories, one is lower esophageal adenocarcinoma, the second is true cardiac adenocarcinoma, and the third is subcardial adenocarcinoma. In western countries, adenocarcinoma of the lower esophagus is the main type, while in my cou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 王立东赵学科宋昕李欣然靳艳秦艳茹常志伟
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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