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Kit for detecting ovarian cancer susceptibility and SNP (single nucleotide polymorphism) marker thereof

A technology of markers and susceptibility, applied in the field of genetic testing, can solve problems such as lack of system integration, limitations, and improper location of materials, and achieve the effects of good technical reproducibility, high practicability, and high success rate

Inactive Publication Date: 2017-02-22
深圳市核子基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have discovered a large number of genetic markers associated with various common diseases. However, due to the lack of sufficient sensitivity and extensive (high-throughput detection sites, high-throughput detection samples) screening for multiple disease-related markers methods of investigation and testing, making the genetic markers of these common diseases not widely available
In addition, the current lack of systematic and effective integration of genetic markers for common diseases greatly restricts the development of early prevention, diagnosis and treatment of common diseases
Existing tests only detect genetic markers for a single disease or a group of diseases, and the detection range is limited, and there is no early detection method for some common diseases
[0005] At present, some traditional medical methods, such as tissue cell detection, have their inherent defects. Improper sampling location, insufficient tissue cell sample materials or lack of experience will lead to misdiagnosis of ovarian cancer
Although other technologies such as imaging have been widely used in the examination and diagnosis of ovarian cancer, they still have great limitations in the qualitative evaluation of the degree of ovarian cancer.

Method used

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  • Kit for detecting ovarian cancer susceptibility and SNP (single nucleotide polymorphism) marker thereof
  • Kit for detecting ovarian cancer susceptibility and SNP (single nucleotide polymorphism) marker thereof
  • Kit for detecting ovarian cancer susceptibility and SNP (single nucleotide polymorphism) marker thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Used to detect SNP sites related to ovarian cancer susceptibility

[0031] Among them, rs2363956 is located in the gene ANKLE1 region, rs8170 is located in the gene BABAM1 region, rs3814113 is located in the gene BNC2 region, rs80356947 and rs80357911 are located in the gene BRCA1 region, rs80358557 is located in the gene BRCA2 region, rs2072590 is located in the gene HOXD3 region, rs12794435 is located in the gene EXOC1 region, rs18 is located in the gene MY882 region rs2084881 and rs9303542 are located in the gene SKAP1 region, rs2665390 is located in the gene TIPARP region, and rs7521902 is located in the gene WNT4 region.

Embodiment 2

[0032] Example 2 Kit for detecting susceptibility to ovarian cancer

[0033] 1. Preparation of the kit:

[0034] 1. Design and synthesize PCR amplification primers and single-base extension primers for the 13 SNP sites. The PCR amplification primers and single base extension primers of the SNP sites to be tested are shown in Table 1

[0035] Table 1 PCR amplification primers and single base extension primers of SNP sites to be tested

[0036]

[0037]

[0038] 2. The kit also includes Taq enzyme, dNTP mixture, MgCl 2 Solution, PCR reaction buffer, enzyme digestion reaction buffer, deionized water.

[0039] 2. The detection method of the kit:

[0040] 1. Extraction of DNA

[0041] 1.1 DNA extraction from oral swab

[0042] 1) Put the oral swab in a 2ml centrifuge tube and add 400μl PBS.

[0043] 2) Add 20 μl QIAGEN Protease and 400 μl Buffer AL. Immediately vortex for 15s to mix. To ensure efficient lysis, sample and Buffer AL must be mixed immediately and thorough...

Embodiment 3

[0089] Example 3 The literature cited by 13 SNP sites closely related to ovarian cancer, see Table 5

[0090] Table 5 Literature cited by 13 SNP sites closely related to ovarian cancer

[0091]

[0092]

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Abstract

The invention discloses a kit for detecting ovarian cancer susceptibility, comprising 13 SNP sites, to be specific, rs2363956, rs8170, rs3814113, rs80356947, rs80357911, rs80358557, rs2072590, rs12794435, rs10088218, rs2084881, rs9303542, rs2665390 and rs7521902, and also discloses PCR (polymerase chain reaction) amplification primer and single-base extension primer of an SNP marker. The 13 SNP sites herein can provide important basis for the assessment and diagnosis of ovarian cancer risk.

Description

technical field [0001] The invention belongs to the field of genetic detection, in particular to a kit for detecting susceptibility to ovarian cancer and its SNP marker. Background technique [0002] Ovarian cancer is one of the common tumors of female reproductive organs, but it is the gynecological malignancy with the highest mortality rate. Because of its hidden onset and rapid progression, most ovarian cancer patients are diagnosed at an advanced stage, and the survival rate is only 20%-30% %, and the survival rate of patients with early ovarian cancer is high, so it is of great significance to improve the early diagnosis of ovarian cancer and improve the prognosis. [0003] Ovarian cancer susceptibility gene detection is mainly based on family history of ovarian cancer and genetic polymorphism diagnostic tests. The association analysis method using single nucleotide polymorphism (Single Nucleotide Polymorphsm, SNP) as a genomic marker is currently one of the most commo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 张核子
Owner 深圳市核子基因科技有限公司
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