Guiding method and kit for sufentanil individualized medication genes

A technology of kit and detection kit, applied in the field of guidance method and kit of sufentanil individualized drug gene

Pending Publication Date: 2021-03-26
广东南芯医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But sufentanil can also be addictive if abused, and

Method used

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  • Guiding method and kit for sufentanil individualized medication genes
  • Guiding method and kit for sufentanil individualized medication genes
  • Guiding method and kit for sufentanil individualized medication genes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Feasibility analysis of SNP site screening of genes related to human sufentanil individualized drug use

[0079] By searching NCBI home and abroad genome-wide association study (genome-wide association study, GWAS) in large-scale pathological control group clinical research verified sufentanil drug safety and effectiveness related sites, the inventors to it After screening and evaluation, 3 single nucleotide polymorphism sites significantly related to sufentanil individualized drug were selected, and independent of each other, there was no linkage disequilibrium, so the site selection of the present invention is representative , independence and risk value accumulation, which can be used to guide the individualized medication of sufentanil.

[0080] The screened SNP sites are as follows:

[0081] rs696, rs2242480, rs1799971.

Embodiment 2

[0082] Embodiment 2 system verification

[0083] System validation includes accuracy, specificity, sensitivity, precision, and comparison among personnel.

[0084] Accuracy verification scheme: 20 cases were detected at each site, compared with Sanger sequencing, the expected target was 95%.

[0085] Specificity Validation Protocol: Included in Accuracy, expected target 95%.

[0086] Sensitive verification scheme: using human genomic DNA positive samples as templates, the DNA contents of calibration samples were 1ng / μL, 5ng / μL, 10ng / μL, 50ng / μL, and 100ng / μL for sensitivity inspection.

[0087] The precision verification plan (including intra-batch, inter-batch, and personnel comparisons, not involving inter-instrument comparisons) has an expected target of 95%.

[0088] Intra-assay precision: The same batch of each sample was repeated 3 times to compare the intra-assay precision.

[0089] Inter-batch precision: The same operator tests the same sample in multiple batches to...

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Abstract

The invention provides a guiding method and kit for sufentanil individualized medication genes. Particularly, the method considers differences of medication doses of different patients, screens out acombination of SNP loci of sufentanil individualized medication related gene, and uses a nucleic acid mass spectrometer for widely screening and inspecting sufentanil-related genetic markers (in a manner of high-throughput detection sites and high-throughput detection samples). The method has a high detection success rate, good technical reproducibility and high cost performance, can realize detection of multiple genes of a single small sample, and meets maximized use of the small sample. The method has technical advantages of high accuracy and high sensitivity, and a stable detection result,and improves a detection positive rate.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a gene guiding method and a kit for sufentanil individualized drug use. Background technique [0002] Single nucleotide polymorphism (single nucleotide polymorphism, SNPs) is a kind of genetic marker, which refers to the polymorphism of DNA sequence caused by single nucleotide variation at the genome level. The frequency of occurrence in the population is greater than 1%, including single-base conversions, inversions, and single-base insertions or deletions. It is a new genetic marker that can be used for disease prediction, diagnosis, treatment, and new drugs. The development provides reliable and effective scientific basis. [0003] SNP occurs in the coding region and can affect protein function, thereby affecting human health or drug metabolism, mainly manifested in individual differences in disease susceptibility, individual differences in the therapeutic ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2521/525C12Q2533/101C12Q2565/627
Inventor 陈涛张静严喜平林金飞
Owner 广东南芯医疗科技有限公司
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