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Kit for detecting esophagus cancer susceptivity and SNP (single nucleotide polymorphism) marker of kit

A technology of susceptibility and markers, applied in the field of genetic testing, can solve problems such as lack of system integration, limitations, and limited detection range, and achieve the effects of good technical reproducibility, high practicability, and high cost performance

Inactive Publication Date: 2017-02-22
深圳市核子基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have discovered a large number of genetic markers associated with various common diseases. However, due to the lack of sufficient sensitivity and extensive (high-throughput detection sites, high-throughput detection samples) screening for multiple disease-related markers methods of investigation and testing, making the genetic markers of these common diseases not widely available
In addition, the current lack of systematic and effective integration of genetic markers for common diseases greatly restricts the development of early prevention, diagnosis and treatment of common diseases
Existing tests only detect genetic markers for a single disease or a group of diseases, and the detection range is limited, and there is no early detection method for some common diseases
[0005] At present, some traditional medical methods, such as tissue cell detection, have their inherent defects. Improper sampling location, insufficient tissue cell sample materials or insufficient experience will lead to misdiagnosis of endometrial cancer
Although other techniques such as imaging have been widely used in the examination and diagnosis of endometrial cancer, they still have great limitations in the qualitative determination of the degree of endometrial cancer.

Method used

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  • Kit for detecting esophagus cancer susceptivity and SNP (single nucleotide polymorphism) marker of kit
  • Kit for detecting esophagus cancer susceptivity and SNP (single nucleotide polymorphism) marker of kit
  • Kit for detecting esophagus cancer susceptivity and SNP (single nucleotide polymorphism) marker of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Used to detect SNP sites associated with susceptibility to esophageal cancer

[0042] Among them, rs10204525 and rs1033667 are located in the region of gene HEATR3, rs1042026 and rs1042522 are located in the region of gene CHEK2, rs10484761 is located in the region of gene ST6GAL1, rs11066015 is located in the region of gene SMG6, rs11066280 is located in the region of gene PTPN2, rs1346044 is located in the region of gene 618ALH1B, 627 is located in the region of gene 618 rs4D27 The gene TMEM173 region, rs1805034 in the gene ATP1B2 region, rs1883965 in the gene HLA region, rs2074356 in the gene PLCE1 region, rs2239612 in the gene MSH2 region, rs2274223 in the gene WRN region, rs238406 in the gene ERCC2 region, rs2847281 in the gene PD-1 region, rs35597309 in the gene region In the ALDH2 region of the gene, rs4785204 is located in the TP53 region of the gene, rs4822983 is located in the RANK region of the gene, rs63749993 is located in the mTOR region of the ge...

Embodiment 2

[0043] Example 2 Kit for detecting susceptibility to esophageal cancer

[0044] 1. Preparation of the kit:

[0045] 1. Design and synthesize PCR amplification primers and single-base extension primers for the 24 SNP sites. The PCR amplification primers and single base extension primers of the SNP sites to be tested are shown in Table 1

[0046] Table 1 PCR amplification primers and single-base extension primers for SNP sites to be tested

[0047]

[0048]

[0049] 2. The kit also includes Taq enzyme, dNTP mixture, MgCl 2 Solution, PCR reaction buffer, enzyme digestion reaction buffer, deionized water.

[0050] 2. The detection method of the kit:

[0051] 1. Extraction of DNA

[0052] 1.1 DNA extraction from oral swab

[0053] 1) Put the oral swab in a 2ml centrifuge tube and add 400μl PBS.

[0054] 2) Add 20 μl QIAGEN Protease and 400 μl Buffer AL. Immediately vortex for 15s to mix. To ensure efficient lysis, sample and Buffer AL must be mixed immediately and th...

Embodiment 3

[0100] Example 3 The literature cited by 24 SNP sites closely related to esophageal cancer, see Table 5

[0101] Table 5 Literature cited by 24 SNP sites closely related to esophageal cancer

[0102]

[0103]

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PUM

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Abstract

The invention discloses an SNP (single nucleotide polymorphism) marker for detecting esophagus cancer susceptivity. The SNP marker comprises 24 SNP loci. The invention further discloses a PCR (polymerase chain reaction) amplification primer, a single-basic-group extension primer and a kit of the SNP marker. An important basis is provided for illness risk evaluation and diagnosis reference of esophagus cancer.

Description

technical field [0001] The invention belongs to the field of genetic detection, in particular to a kit for detecting the susceptibility of esophageal cancer and its SNP marker. Background technique [0002] Esophageal cancer is a malignant tumor of the esophageal mucosal epithelium and esophageal glandular epithelium. Or a large number of internal and external studies have shown that the incidence of esophageal cancer is related to genetic factors. [0003] The association analysis method using single nucleotide polymorphism (Single Nucleotide Polymorphsm, SNP) as a genomic marker is currently one of the most commonly used methods for detecting genetic susceptibility genes of diseases. SNP refers to the DNA sequence polymorphism caused by single nucleotide variation at the genome level. The frequency of occurrence in the population is greater than 1%. It is the most common type of human heritable variation. SNPs have strong genetic stability and are easy to detect. SNPs lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 张核子
Owner 深圳市核子基因科技有限公司
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