Kit for detecting nasopharyngeal cancer susceptibility and SNP marker of kit

A technology of susceptibility and markers, applied in the field of genetic testing, can solve the problems of lack of system integration, improper sampling location, limited detection range, etc., and achieve the effect of good technical reproducibility, high practicability, and high cost performance

Inactive Publication Date: 2017-05-31
深圳市核子基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have discovered a large number of genetic markers associated with various common diseases. However, due to the lack of sufficient sensitivity and extensive (high-throughput detection sites, high-throughput detection samples) screening for multiple disease-related markers methods of investigation and testing, making the genetic markers of these common diseases not widely available
In addition, the current lack of systematic and effective integration of genetic markers for common diseases greatly restricts the development of early prevention, diagnosis and treatment of common diseases
Existing tests only detect genetic markers for a single disease or a group of diseases, and the detection range is limited, and there is no early detection method for some common diseases
[0005] At present, some traditional medical methods, such as tissue cell detection, have their inherent defects. Improper sampling location, insufficient tissue cell sample material or lack of experience will lead to misdiagnosis of nasopharyngeal carcinoma
Although other techniques such as imaging have been widely used in the examination and diagnosis of nasopharyngeal carcinoma, there are still great limitations in the qualitative determination of the degree of nasopharyngeal carcinoma

Method used

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  • Kit for detecting nasopharyngeal cancer susceptibility and SNP marker of kit
  • Kit for detecting nasopharyngeal cancer susceptibility and SNP marker of kit
  • Kit for detecting nasopharyngeal cancer susceptibility and SNP marker of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Used to detect SNP sites associated with susceptibility to nasopharyngeal carcinoma

[0039] 其中rs9344位于基因CCND1区域;rs13347位于基因CD44区域;rs1412829位于基因CDKN2A区域;rs9418990、rs915906、rs8192780、rs3827688、rs3813865、rs2249695和rs1536826位于基因CYP2E1区域;rs29232位于基因GABBR1区域;rs2517713和rs2975042位于基因HLA- A region; rs3129055 and rs3131866 are located in the gene HLA-F region; rs11556218 is located in the gene IL16 region; rs10889677 is located in the gene IL23R region; rs6774494 is located in the gene MDS1-EVI1 region; rs243865 and rs2285053 are located in the gene MMP2 region; rs9510787 is located in the gene TNFRSF19 region.

Embodiment 2

[0040] Example 2 Kit for detecting susceptibility to nasopharyngeal carcinoma

[0041] 1. Preparation of the kit:

[0042] 1. Design and synthesize PCR amplification primers and single-base extension primers for the 21 SNP sites. The PCR amplification primers and single base extension primers of the SNP sites to be tested are shown in Table 1

[0043] Table 1 PCR amplification primers and single-base extension primers for SNP sites to be tested

[0044]

[0045]

[0046] 2. The kit also includes Taq enzyme, dNTP mixture, MgCl 2 Solution, PCR reaction buffer, enzyme digestion reaction buffer, deionized water.

[0047] 2. The detection method of the kit:

[0048] 1. Extraction of DNA

[0049] 1.1 DNA extraction from oral swab

[0050] 1) Put the oral swab in a 2ml centrifuge tube and add 400μl PBS.

[0051] 2) Add 20 μl QIAGEN Protease and 400 μl Buffer AL. Immediately vortex for 15s to mix. To ensure efficient lysis, sample and Buffer AL must be mixed immediately...

Embodiment 3

[0097] Example 3 The literature cited by 21 SNP sites closely related to nasopharyngeal carcinoma, see Table 5

[0098] Table 5 Literature cited by 21 SNP sites closely related to nasopharyngeal carcinoma

[0099]

[0100]

[0101]

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PUM

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Abstract

The invention discloses an SNP marker for detecting nasopharyngeal cancer susceptibility. The SNP marker comprises 21 SNP loci: rs9344, rs13347, rs1412829, rs9418990, rs915906, rs8192780, rs3827688, rs3813865, ra2249695, rs1536826, rs29232, rs2517713, rs2975042, rs3129055, rs3131866, rs22556218, rs10889677, rs6774494, rs243865, rs2285053 and rs9510787. The invention furthermore discloses a PCR amplification primer, a single-base extension primer and a kit of the SNP marker, so as to provide important basis for the illness risk assessment and diagnosis reference of nasopharyngeal cancer.

Description

technical field [0001] The invention belongs to the field of genetic detection, in particular to a kit for detecting the susceptibility of nasopharyngeal carcinoma and its SNP marker. Background technique [0002] Nasopharyngeal carcinoma (NPC) is an idiopathic malignant tumor of the head and neck. Statistical data analysis shows that NPC patients have racial and family clustering phenomenon, and the high-incidence area still maintains a high incidence after moving to other areas, and 10% of NPC patients have a family history of cancer, etc., suggesting genetic susceptibility / Genetic predisposition may be an important factor in the pathogenesis of nasopharyngeal carcinoma. [0003] The association analysis method using single nucleotide polymorphism (Single Nucleotide Polymorphsm, SNP) as a genomic marker is currently one of the most commonly used methods for detecting genetic susceptibility genes of diseases. SNP refers to the DNA sequence polymorphism caused by single ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 张核子
Owner 深圳市核子基因科技有限公司
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