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Method for catalytic synthesis of vitamin A fatty acid ester using immobilized lipase

A technology of immobilizing lipase and fatty acid ester, which is applied in fermentation and other fields, can solve the problems of complex catalyst preparation, difficult separation and purification, and environmental pollution, so as to improve catalytic activity and thermodynamic stability, strong reaction specificity, and reduce The effect of production costs

Inactive Publication Date: 2005-06-01
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of the catalyst in this method is complicated and the cost is high; and the organic solvent benzene is used as the reaction medium, and benzene is toxic and pollutes the environment
Moreover, using too much fatty acid will increase the difficulty of subsequent separation and purification, which will inevitably lead to high product cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028]Mix the cotton cloth and the co-fixative in a ratio of 1:1 by mass to volume (W:V), dry at room temperature to obtain an activated carrier; the co-fixatives are gelatin, lecithin, polyethylene glycol, polysorbate, fatty acid The mixture of sorbitan and magnesium salt, its mass ratio is: gelatin: lecithin: polyethylene glycol: polysorbate: fatty acid sorbitan: magnesium chloride=5:1:1:2:2:1; The aqueous solution of silk yeast lipase is soaked with the activated carrier at a ratio of 8000 units / gram activated carrier, mixed, and dried at room temperature to obtain an immobilized lipase whose enzyme activity is 8000IU / g; then substrate 0.100g (0.3mmol) Retinoic acid ester and 0.234g (0.9mmol) palmitic acid and 10ml water content are 0.5% normal hexane solvent and 0.3g immobilized lipase mixing, under the condition of temperature 30 ℃, in the shaker (190r / min), After 24 hours of reaction, the conversion rate was 79%. The immobilized lipase was taken out, and the reaction sol...

Embodiment 2

[0030] The operation steps are the same as those in Example 1, except that the resin is used instead of cotton cloth; Methylolytica lipase is used instead of M. antarctica; the conversion rate is 82%.

Embodiment 3

[0032] The operation steps are the same as in Example 1, except that the cotton cloth is made of diatomaceous earth; the conversion rate is 73%.

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PUM

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Abstract

The method of catalytically synthesizing vitamin A fatty ester with immobilized lipase includes mixing substrate vitamin An acetate and C10-C18 fatty acid or fatty ester in the molar ratio of 1 to 1-7 as well as organic solvent and immobilized lipase in the amount of 0.2-5 times weight of vitamin An acetate for reaction under 20-50 deg.c for 9-50 hr; taking out the immobilized lipase; filtering the reacted liquid and crystallizing to obtain vitamin A fatty ester. The organic solvent is C6-C10 saturated alkane containing water in 0-1 %; and the immobilized lipase is yeast type lipase on carrier of diatomite, resin or fabric membrane. The immobilized lipase of the present invention has the features of great catalysis area, high catalysis efficiency, high stability, capacity of being reused, etc. The reaction solvent has no toxicity, and the product is light in color and high in quality.

Description

(1) Technical field [0001] The invention relates to a method for synthesizing vitamin A fatty acid esters by reacting vitamin A acetate with long-chain fatty acids or long-chain fatty acid esters, especially synthesizing vitamin A fats by using immobilized microbial lipase to catalyze the reaction in a non-aqueous phase system ester method. (2) Background technology [0002] Vitamin A fatty acid ester is a chemical substance commonly used in many daily chemical industries such as food, medicine, nutritional health products, cosmetics, feed additives, etc. It is an indispensable substance for the normal metabolism of the human body. It can maintain and promote the growth and development of the human body , reproduction and the stability of the cell membrane have a significant effect on the formation of vision. Moreover, vitamin A fatty acid esters also help cells multiply and distinguish necrotic cells, and act as an antioxidant to combine with free radicals that can cause g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00
Inventor 谭天伟刘涛尹春华
Owner BEIJING UNIV OF CHEM TECH
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