Culture medium for producing benzoylformic acid by fermentation method

A technology of acetophenone and culture medium, applied in the direction of fermentation, etc., can solve the problems of molecular shape influence, low biomass, substrate concentration and conversion rate can not continue to increase, and achieve the effect of convenient operation and high reaction specificity

Inactive Publication Date: 2009-06-24
SHANGHAI INST OF PHARMA IND CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The disadvantage of this method is that the biomass during the transformation culture is low; when the initial pH is 7.0, the pH value during the transformation process is maintained near the isoelectric point of phenylglycine, which affects both the solubility of the substrate and the molecular shape of the amino group of the substrate ; Calcium ion concentration 0.0002wt% is then on the low side in the original conversion culture medium, can not have optimal action effect, therefore, cause substrate concentration and conversion rate to be unable to continue to improve

Method used

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  • Culture medium for producing benzoylformic acid by fermentation method
  • Culture medium for producing benzoylformic acid by fermentation method
  • Culture medium for producing benzoylformic acid by fermentation method

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Experimental program
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Effect test

Embodiment 1

[0028] Candida lipolytica SIPI0201 bacterial classification was inoculated in 25ml seed medium (glucose 3wt%, yeast extract 0.3wt%,, peptone 0.3wt%, K 2 HPO 4 ·3H 2 O 0.1wt%, MgSO 4 ·7H 2(0.05wt%, KCl 0.05wt%, natural pH), 30 ℃, 230r / min aerobic culture 20h, obtain seed liquid, then insert 25ml fermentation medium (fructose 0.2wt%, peptone 0.7 wt%, (NH 4 ) 2 SO 4 0.1wt%, KH 2 PO 4 0.4wt%, yeast extract 0.3wt%, calcium chloride 0.0003wt%, D, L-phenylglycine 2wt%, adjust the pH value to 8.0 with NaOH), aerobic culture under the same conditions for 6 days, HPLC analysis of acetophenone The yield was 35.9%.

Embodiment 2

[0030] Candida lipolytica SIPI0201 bacterial classification was inoculated in 25ml seed medium (glucose 3wt%, yeast extract 0.3wt%,, peptone 0.3wt%, K 2 HPO 4 ·3H 2 O 0.1wt%, MgSO 4 ·7H 2 (0.05wt%, KCl 0.05wt%, natural pH), 30 ℃, 230r / min aerobic culture 20h, obtain seed liquid, then insert 25ml fermentation medium (fructose 0.2wt%, peptone 0.7 wt%, (NH 4 ) 2 SO 4 0.1wt%, KH 2 PO 4 0.4wt%, yeast extract 1.2wt%, calcium chloride 0.005wt%, D, L-phenylglycine 2wt%, adjust the pH value to 7.5 with NaOH), aerobic culture under the same conditions for 6 days, HPLC analysis of acetophenone The yield was 39.5%.

Embodiment 3

[0032] Candida lipolytica SIPI0201 bacterial classification was inoculated in 25ml seed medium (glucose 3wt%, yeast extract 0.3wt%,, peptone 0.3wt%, K 2 HPO 4 ·3H 2 O 0.1wt%, MgSO 4 ·7H 2 (0.05wt%, KCl 0.05wt%, natural pH), 30 ℃, 230r / min aerobic culture 20h, obtain seed liquid, then insert 25ml fermentation medium (fructose 0.2wt%, peptone 0.7 wt%, (NH 4 ) 2 SO 4 0.1wt%, KH 2 PO 4 0.4wt%, yeast extract 2.4wt%, calcium chloride 0.005wt%, D, L-phenylglycine 2wt%, adjust the pH value to 8.0 with NaOH), aerobic culture under the same conditions for 6 days, HPLC analysis of acetophenone The yield was 37.7%.

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Abstract

The invention discloses a culture medium for producing benzoylformic acid by fermentation method. The culture medium comprises calcium ion and D, L-phenylglycine and is characterized in that the initial pH value of the culture medium ranges from pH more than 7 to pH equal to 10. By adopting the culture medium to produce the benzoylformic acid, the substrate inventory rating can increase to 50g / L; the conversion rate can reach 36.8%, the response-specific is high, almost no byproduct exists, simple extraction is enough, and the operation is very convenient.

Description

technical field [0001] The invention belongs to the field of microbial fermentation engineering, in particular, the invention relates to a culture medium for producing acetophenone by fermentation. Background technique [0002] Phenylglyoxilic acid, also known as benzoylformic acid, is an important intermediate in the synthesis of some pesticides and pharmaceutical products, and can be used to synthesize the herbicide fenmetrione and the pharmaceutical product Weichangning, etc. R(-)-mandelic acid is an extremely important chiral pharmaceutical intermediate, which is widely used in the asymmetric synthesis of optically pure amino acids, angiotensin converting enzyme inhibitors, and coenzyme A. At present, domestic studies have used acetophenone acid as a substrate to asymmetrically synthesize R(-)-mandelic acid. But the preparation method of acetophenone acid mainly contains two kinds of methods, benzoyl cyanide hydrolysis method and styrene oxidation method at present, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40
Inventor 胡海峰徐梅张琴
Owner SHANGHAI INST OF PHARMA IND CO LTD
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