Improved rotating packed bed reactor for enzyme catalysis and application of reactor in fatty acid preparation through enzyme catalysis lipid hydrolysis

Abstract

The invention discloses a rotating packed bed reactor for enzyme catalysis and an application method of the reactor in fatty acid production through enzyme catalysis lipid hydrolysis. The method comprises the steps that lipid and water are adopted as raw materials to be mixed with lipase for a catalysis reaction, the catalysis reaction is conducted under the condition that the supergravity level (gravitational acceleration) is 5 g to 120 g, steel mesh packing is adopted, the substrate flow speed is 80 mL / min to 120 mL / min, the temperature is 35 DEG C to 55 DEG C, the lipase adding amount (lipase / substrates) is 0.5 wt% to 1.5 wt%, and the lipid-water mass ratio is (0.5-1.5):1, free fatty acid is prepared after reacting is conducted for 8 hours to 24 hours, and the hydrolysis yield can reach 98%. According to the method, lipase is used for catalytically producing the fatty acid in the rotating packed bed reactor for the first time, and the advantages that a rotating packed bed is high in mass transfer intensity and mass transfer efficiency, and the materials are full in micromixing in the bed and short in dwell time are achieved in a lipase catalysis technology; in a solvent-free system, compared with a traditional stirring system, the technology has the advantages that the reaction time is shortened by 30%-50%, and the fatty acid yield is increased by 15% to 50%.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and specifically relates to an improved rotating packed bed reactor with high-efficiency mass transfer and microscopic mixing and its application in enzyme-catalyzed oil hydrolysis to prepare fatty acids. Background technique [0002] The high-gravity rotating bed simulates the high-gravity environment through the centrifugal force generated by the rotation of the rotor. This instrument can greatly enhance the mass transfer and the micro-mixing between materials. The packed bed type, spiral channel type, spiral type and other high gravity rotating bed reactors in the high gravity rotating bed device have been disclosed in patents (application numbers 91111028.3, 91109255.2, 01268009.5, 200520100685.3, 02114174.6 and 200510032296.6). At present, this technology has been applied in many fields such as separation, desorption, chemical reaction, and ultrafine particle preparation (patent...

AI Technical Summary

Problems solved by technology

[0004] Traditional methods of oil hydrolysis to generate fatty acids mainly include physical, chemical, and biological enzyme catalysis. Unsaturated fatty acids are prone to decomposition and rearrangement reactions, consume a large amount of acid and alkali, and cause serious environmental pollution, which cannot meet the needs of high-quality products

The microscopic mixing effect of the enzyme-catalyzed substrate in the traditional reactor is poor, and the particle size distribution is uneven; while in the reaction environment of the rotating packed bed, the microscopic mixing is better, the particle size distribution is uniform, and the high mass transfer effect also increases the enzyme and substrate. chance of contact

Images